Methods and compositions for weed control

ABSTRACT

Novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate protoporphyrinogen IX oxidase in weed species. The present invention also provides for combinations of compositions and methods that enhance weed control.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of U.S. application Ser. No. 13/612,985 filed on Sep. 13, 2012, which claims the benefit and priority of U.S. Provisional Patent Application No. 61/534,086 filed on Sep. 13, 2011, each of which is herein incorporated by reference in its entirety. The sequence listing that is contained in the file named “P34115US02_Sequence_listing_09282016.txt,” which is 807,910 bytes (measured in operating system MS Windows) and was created on Sep. 28, 2016, is filed herewith and incorporated herein by reference.

FIELD

The methods and compositions generally relate to the field of weed management. More specifically, the methods and compositions relate to protoporphyrinogen IX oxidase (PPG oxidase) genes in plants and compositions containing polynucleotide molecules for modulating and/or regulating their expression. Further provided are methods and compositions useful for weed control.

BACKGROUND

Weeds are plants that compete with cultivated plants in an agronomic environment and cost farmers billions of dollars annually in crop losses and the expense of efforts to keep weeds under control. Weeds also serve as hosts for crop diseases and insect pests. The losses caused by weeds in agricultural production environments include decreases in crop yield, reduced crop quality, increased irrigation costs, increased harvesting costs, reduced land value, injury to livestock, and crop damage from insects and diseases harbored by the weeds. The principal means by which weeds cause these effects are: 1) competing with crop plants for water, nutrients, sunlight and other essentials for growth and development, 2) production of toxic or irritant chemicals that cause human or animal health problem, 3) production of immense quantities of seed or vegetative reproductive parts or both that contaminate agricultural products and perpetuate the species in agricultural lands, and 4) production on agricultural and nonagricultural lands of vast amounts of vegetation that must be disposed of Herbicide tolerant weeds are a problem with nearly all herbicides in use, there is a need to effectively manage these weeds. There are over 365 weed biotypes currently identified as being herbicide resistant to one or more herbicides by the Herbicide Resistance Action Committee (HRAC), the North American Herbicide Resistance Action Committee (NAHRAC), and the Weed Science Society of America (WSSA).

BRIEF DESCRIPTION OF THE FIGURES

The following drawings form part of the present specification and are included to further demonstrate certain methods, compositions or results. They may be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein. The invention can be more fully understood from the following description of the figures:

FIG. 1. Treatment of Amaranthus palmeri with ssDNA trigger polynucleotides and PPG oxidase inhibitor herbicide, flumioxazin.

FIG. 2. Treatment of Amaranthus palmeri with ssDNA trigger polynucleotides and PPG oxidase inhibitor herbicide, fomesafen.

FIG. 3. Treatment of Amaranthus palmeri with pooled oligos and ssDNA trigger polynucleotides and PPG oxidase inhibitor herbicide, Reflex® (fomesafen).

SUMMARY

In one aspect, the invention provides a method of plant control comprising an external application to a plant of a composition comprising a polynucleotide and a transfer agent, wherein the polynucleotide is essentially identical or essentially complementary to a PPG oxidase gene sequence or fragment thereof, or to the RNA transcript of said PPG oxidase gene sequence or fragment thereof, wherein said PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs:1-71 and a polynucleotide fragment thereof, whereby the plant growth or development or reproductive ability is reduced or the weedy plant is made more sensitive to a PPG oxidase inhibitor herbicide relative to a plant not treated with said composition. In this manner, plants that have become resistant to the application of PPG oxidase inhibitor containing herbicides may be made more susceptible to the herbicidal effects of a PPG oxidase inhibitor containing herbicide, thus potentiating the effect of the herbicide. The polynucleotide fragment is at least 18 contiguous nucleotides, at least 19 contiguous nucleotides, at least 20 contiguous nucleotides or at least 21 contiguous nucleotides in length and at least 85 percent identical to a PPG oxidase gene sequence selected from the group consisting of SEQ ID NOs:1-71 and the transfer agent is an organosilicone composition or compound. The polynucleotide fragment can also be sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA, or dsDNA/RNA hybrids. The composition can include more than one polynucleotide fragments, and the composition can include a PPG oxidase inhibitor herbicide and/or other herbicides that enhance the weed control activity of the composition.

In another aspect, polynucleotide molecules and methods for modulating PPG oxidase gene expression in weedy plant species are provided. The method reduces, represses or otherwise delays expression of a PPG oxidase gene in a weedy plant comprising an external application to a weedy plant of a composition comprising a polynucleotide and a transfer agent, wherein the polynucleotide is essentially identical or essentially complementary to a PPG oxidase gene sequence or fragment thereof, or to the RNA transcript of the PPG oxidase gene sequence or fragment thereof, wherein the PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs:1-71 and a polynucleotide fragment thereof. The polynucleotide fragment is at least 18 contiguous nucleotides, at least 19 contiguous nucleotides, at least 20 contiguous nucleotides, at least 21 contiguous nucleotides in length and at least 85 percent identical to a PPG oxidase gene sequence selected from the group consisting of SEQ ID NOs:1-71 and the transfer agent is an organosilicone compound. The polynucleotide fragment can also be sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA, or dsDNA/RNA hybrids.

In a further aspect, the polynucleotide molecule containing composition may be combined with other herbicidal compounds to provide additional control of unwanted plants in a field of cultivated plants.

In a further aspect, the polynucleotide molecule composition may be combined with any one or more additional agricultural chemicals, such as insecticides, fungicides, nematocides, bactericides, acaricides, growth regulators, chemosterilants, semiochemicals, repellents, attractants, pheromones, feeding stimulants, biopesticides, microbial pesticides or other biologically active compounds to form a multi-component pesticide giving an even broader spectrum of agricultural protection.

DETAILED DESCRIPTION

Provided are methods and compositions containing a polynucleotide that provide for regulation, repression or delay of PPG oxidase (protoporphyrinogen IX oxidase) gene expression and enhanced control of weedy plant species and importantly PPG oxidase inhibitor resistant weed biotypes. Aspects of the method can be applied to manage various weedy plants in agronomic and other cultivated environments.

The following definitions and methods are provided to better define the present invention and to guide those of ordinary skill in the art in the practice of the present invention. Unless otherwise noted, terms are to be understood according to conventional usage by those of ordinary skill in the relevant art. Where a term is provided in the singular, the inventors also contemplate aspects of the invention described by the plural of that term.

By “non-transcribable” polynucleotides is meant that the polynucleotides do not comprise a complete polymerase II transcription unit.

As used herein “solution” refers to homogeneous mixtures and non-homogeneous mixtures such as suspensions, colloids, micelles, and emulsions.

Weedy plants are plants that compete with cultivated plants, those of particular importance include, but are not limited to, important invasive and noxious weeds and herbicide resistant biotypes in crop production, such as Amaranthus species—A. albus, A. blitoides, A. hybridus, A. palmeri, A. powellii, A. retroflexus, A. spinosus, A. tuberculatus, and A. viridis; Ambrosia species—A. trifida, A. artemisifolia; Lolium species—L. multiflorum, L. rigidium, L perenne; Digitaria species—D. insularis; Euphorbia species—E. heterophylla; Kochia species—K. scoparia; Sorghum species—S. halepense; Conyza species—C. bonariensis, C. canadensis, C. sumatrensis; Chloris species—C. truncate; Echinochloa species—E. colona, E. crus-galli; Eleusine species—E. indica; Poa species—P. annua; Plantago species—P. lanceolata; Avena species—A. fatua; Chenopodium species—C. album; Setaria species—S. viridis, Abutilon theophrasti; Ipomoea species; Sesbania species; Cassia species; Sida species; Brachiaria species; and Solanum species.

Additional weedy plant species found in cultivated areas include Alopecurus myosuroides, Avena sterilis, Avena sterilis ludoviciana, Brachiaria plantaginea, Bromus diandrus, Bromus rigidus, Cynosurus echinatus, Digitaria ciliaris, Digitaria ischaemum, Digitaria sanguinalis, Echinochloa oryzicola, Echinochloa phyllopogon, Eriochloa punctata, Hordeum glaucum, Hordeum leporinum, Ischaemum rugosum, Leptochloa chinensis, Lolium persicum, Phalaris minor, Phalaris paradoxa, Rottboellia exalta, Setaria faberi, Setaria viridis var, robusta-alba schreiber, Setaria viridis var, robusta-purpurea, Snowdenia polystachea, Sorghum sudanese, Alisma plantago-aquatica, Amaranthus lividus, Amaranthus quitensis, Ammania auriculata, Ammania coccinea, Anthemis cotula, Apera spica-venti, Bacopa rotundifolia, Bidens pilosa, Bidens subalternans, Brassica tournefortii, Bromus tectorum, Camelina microcarpa, Chrysanthemum coronarium, Cuscuta campestris, Cyperus difformis, Damasonium minus, Descurainia sophia, Diplotaxis tenuifolia, Echium plantagineum, Elatine triandra var, pedicellata, Euphorbia heterophylla, Fallopia convolvulus, Fimbristylis miliacea, Galeopsis tetrahit, Galium spurium, Helianthus annuus, Iva xanthifolia, Ixophorus unisetus, Ipomoea indica, Ipomoea purpurea, Ipomoea sepiaria, Ipomoea aquatic, Ipomoea triloba, Lactuca serriola, Limnocharis flava, Limnophila erecta, Limnophila sessiliflora, Lindernia dubia, Lindernia dubia var major, Lindernia micrantha, Lindernia procumbens, Mesembryanthemum crystallinum, Monochoria korsakowii, Monochoria vaginalis, Neslia paniculata, Papaver rhoeas, Parthenium hysterophorus, Pentzia suffruticosa, Phalaris minor, Raphanus raphanistrum, Raphanus sativus, Rapistrum rugosum, Rotala indica var, uliginosa, Sagittaria guyanensis, Sagittaria montevidensis, Sagittaria pygmaea, Salsola iberica, Scirpus juncoides var ohwianus, Scirpus mucronatus, Setaria lutescens, Sida spinosa, Sinapis arvensis, Sisymbrium orientale, Sisymbrium thellungii, Solanum ptycanthum, Sonchus aspen, Sonchus oleraceus, Sorghum bicolor, Stellaria media, Thlaspi arvense, Xanthium strumarium, Arctotheca calendula, Conyza sumatrensis, Crassocephalum crepidiodes, Cuphea carthagenenis, Epilobium adenocaulon, Erigeron philadelphicus, Landoltia punctata, Lepidium virginicum, Monochoria korsakowii, Solanum americanum, Solanum nigrum, Vulpia bromoides, Youngia japonica, Hydrilla verticillata, Carduus nutans, Carduus pycnocephalus, Centaurea solstitialis, Cirsium arvense, Commelina diffusa, Convolvulus arvensis, Daucus carota, Digitaria ischaemum, Echinochloa crus-pavonis, Fimbristylis miliacea, Galeopsis tetrahit, Galium spurium, Limnophila erecta, Matricaria perforate, Papaver rhoeas, Ranunculus acris, Soliva sessilis, Sphenoclea zeylanica, Stellaria media, Nassella trichotoma, Stipa neesiana, Agrostis stolonifera, Polygonum aviculare, Alopecurus japonicus, Beckmannia syzigachne, Bromus tectorum, Chloris inflate, Echinochloa erecta, Portulaca oleracea, and Senecio vulgaris. It is believed that all plants contain a protoporphyrinogen IX oxidase gene in their genome, the sequence of which can be isolated, and polynucleotides made according to the methods of the present invention that are useful for regulating, suppressing or delaying the expression of the target PPG oxidase gene in the plants and the growth or development of the treated plants.

Some cultivated plants may also be weedy plants when they occur in unwanted environments. Transgenic crops with one or more herbicide tolerances will need specialized methods of management to control weeds and volunteer crop plants and to target the herbicide tolerance transgene as necessary to permit the treated plants to become sensitive to the herbicide.

A “trigger” or “trigger polynucleotide” is a polynucleotide molecule that is homologous or complementary to a target gene polynucleotide. The trigger polynucleotide molecules modulate expression of the target gene when topically applied to a plant surface with a transfer agent, whereby a plant treated with said composition has its growth or development or reproductive ability regulated, suppressed or delayed or said plant is more sensitive to a PPG oxidase inhibitor herbicide or mitosis inhibitor herbicide as a result of said polynucleotide containing composition relative to a plant not treated with a composition containing the trigger molecule. Trigger polynucleotides disclosed herein are generally described in relation to the target gene sequence and may be used in the sense (homologous) or antisense (complementary) orientation as single stranded molecules or comprise both strands as double stranded molecules or nucleotide variants and modified nucleotides thereof depending on the various regions of a gene being targeted.

It is contemplated that the composition of the present invention will contain multiple polynucleotides and herbicides that include but are not limited to PPG oxidase gene trigger polynucleotides and a PPG oxidase inhibitor herbicide and any one or more additional herbicide target gene trigger polynucleotides and the related herbicides and any one or more additional essential gene trigger polynucleotides. Essential genes are genes in a plant that provide key enzymes or other proteins, for example, a biosynthetic enzyme, metabolizing enzyme, receptor, signal transduction protein, structural gene product, transcription factor, or transport protein; or regulating RNAs, such as microRNAs, that are essential to the growth or survival of the organism or cell or involved in the normal growth and development of the plant (Meinke et al., Trends Plant Sci., 2008 September; 13(9):483-91). The suppression of an essential gene enhances the effect of a herbicide that affects the function of a gene product different than the suppressed essential gene. The compositions of the present invention can include various trigger polynucleotides that modulate the expression of an essential gene other than PPG oxidase.

Herbicides for which transgenes for plant tolerance have been demonstrated and the method of the present invention can be applied, include but are not limited to: auxin-like herbicides, glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon, dicamba, cyclohezanedione, protoporphyrinogen oxidase inhibitors, 4-hydroxyphenyl-pyruvate-dioxygenase inhibitors herbicides. For example, transgenes and their polynucleotide molecules that encode proteins involved in herbicide tolerance are known in the art, and include, but are not limited to a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), for example, as more fully described in U.S. Pat. No. 7,807,791 (SEQ ID NO:5); U.S. Pat. Nos. 6,248,876 B1; 5,627,061; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,462,910; 5,188,642; 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,460,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; Re. 37,287 E; and 5,491,288; tolerance to sulfonylurea and/or imidazolinone, for example, as described more fully in U.S. Pat. Nos. 5,605,011; 5,013,659; 5,141,870; 5,767,361; 5,746,180; 5,304,732; 4,761,373; 5,346,107; 5,928,937; and 5,378,824; and international publication WO 96/33270; tolerance to hydroxyphenylpyruvatedioxygenases inhibiting herbicides in plants are described in U.S. Pat. Nos. 6,245,968 B1; 6,268,549; and U.S. Pat. Nos. 6,069,115; 7,462,379 (SEQ ID NO:3); U.S. Pat. Nos. 7,935,869; 7,304,209 (SEQ ID NOs:1, 3, 5 and 15); and US Pat. Pub. 20110191897; aryloxyalkanoate dioxygenase polynucleotides, which confer tolerance to 2,4-D and other phenoxy auxin herbicides as well as to aryloxyphenoxypropionate herbicides as described, for example, in WO2005/107437; U.S. Pat. No. 7,838,733 (SEQ ID NO:5); and dicamba-tolerance polynucleotides as described, for example, in Herman et al. (2005), J. Biol. Chem., 280: 24759-24767. Other examples of herbicide-tolerance traits include those conferred by polynucleotides encoding an exogenous phosphinothricin acetyltransferase, as described in U.S. Pat. Nos. 5,969,213; 5,489,520; 5,550,468; 5,874,265; 5,919,675; 5,561,236; 5,648,477; 5,646,024; 6,177,616; and 5,879,903. Plants containing an exogenous phosphinothricin acetyltransferase can exhibit improved tolerance to glufosinate herbicides, which inhibit the enzyme glutamine synthase. Additionally, herbicide-tolerance polynucleotides include those conferred by polynucleotides conferring altered protoporphyrinogen oxidase (protox) activity, as described in U.S. Pat. Nos. 6,288,306 B1; 6,282,837 B1; and 5,767,373; and WO 01/12825. Plants containing such polynucleotides can exhibit improved tolerance to any of a variety of herbicides which target the protox enzyme (also referred to as protox inhibitors). Polynucleotides encoding a glyphosate oxidoreductase and a glyphosate-N-acetyl transferase (GOX described in U.S. Pat. No. 5,463,175 and GAT described in U.S. Patent publication 20030083480, dicamba monooxygenase described in U.S. Patent publication 20030135879, all of which are incorporated herein by reference); a polynucleotide molecule encoding bromoxynil nitrilase (Bxn described in U.S. Pat. No. 4,810,648 for Bromoxynil tolerance, which is incorporated herein by reference); a polynucleotide molecule encoding phytoene desaturase (crtI) described in Misawa et al. (1993), Plant J., 4:833-840, and Misawa et al. (1994), Plant J., 6:481-489, for norflurazon tolerance; a polynucleotide molecule encoding acetohydroxyacid synthase (AHAS, aka ALS) described in Sathasiivan et al. (1990), Nucl. Acids Res., 18:468-2193, for tolerance to sulfonylurea herbicides; and the bar gene described in DeBlock et al. (1987), EMBO J., 6:2513-2519, for glufosinate and bialaphos tolerance. The transgenic coding regions and regulatory elements of the herbicide tolerance genes are targets in which polynucleotide triggers and herbicides can be included in the composition of the present invention.

The composition includes a component that is a PPG oxidase inhibitor herbicide, which includes but is not limited to acifluorfen-Na, bifenox, chlomethoxyfen, fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen, fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin, flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon, oxadiargyl, azafenidin, carfentrazone-ethyl, sulfentrazone, pentoxazone, benzfendizone, butafenacil, pyrazogyl, and profluazol.

Numerous additional herbicides with similar or different modes of action (herein referred to as co-herbicides) are available that can be added to the composition, for example, members of the herbicide families that include but are not limited to amide herbicides, aromatic acid herbicides, arsenical herbicides, benzothiazole herbicides, benzoylcyclohexanedione herbicides, benzofuranyl alkylsulfonate herbicides, carbamate herbicides, cyclohexene oxime herbicides, cyclopropylisoxazole herbicides, dicarboximide herbicides, dinitroaniline herbicides, dinitrophenol herbicides, diphenyl ether herbicides, dithiocarbamate herbicides, halogenated aliphatic herbicides, imidazolinone herbicides, inorganic herbicides, nitrile herbicides, organophosphorus herbicides, oxadiazolone herbicides, oxazole herbicides, phenoxy herbicides, phenylenediamine herbicides, pyrazole herbicides, pyridazine herbicides, pyridazinone herbicides, pyridine herbicides, pyrimidinediamine herbicides, pyrimidinyloxybenzylamine herbicides, quaternary ammonium herbicides, thiocarbamate herbicides, thiocarbonate herbicides, thiourea herbicides, triazine herbicides, triazinone herbicides, triazole herbicides, triazolone herbicides, triazolopyrimidine herbicides, uracil herbicides, and urea herbicides. In particular, the rates of use of the added herbicides can be reduced in compositions comprising the polynucleotides. Contemplated use rate reductions of the additional added herbicides can be 10-25 percent, 26-50 percent, 51-75 percent or more, and can be achieved from the enhanced activity of the polynucleotides and herbicide composition. Representative co-herbicides of the families include but are not limited to acetochlor, acifluorfen, acifluorfen-sodium, aclonifen, acrolein, alachlor, alloxydim, allyl alcohol, ametryn, amicarbazone, amidosulfuron, aminopyralid, amitrole, ammonium sulfamate, anilofos, asulam, atraton, atrazine, azimsulfuron, BCPC, beflubutamid, benazolin, benfluralin, benfuresate, bensulfuron, bensulfuron-methyl, bensulide, bentazone, benzfendizone, benzobicyclon, benzofenap, bifenox, bilanafos, bispyribac, bispyribac-sodium, borax, bromacil, bromobutide, bromoxynil, butachlor, butafenacil, butamifos, butralin, butroxydim, butylate, cacodylic acid, calcium chlorate, cafenstrole, carbetamide, carfentrazone, carfentrazone-ethyl, CDEA, CEPC, chlorflurenol, chlorflurenol-methyl, chloridazon, chlorimuron, chlorimuron-ethyl, chloroacetic acid, chlorotoluron, chlorpropham, chlorsulfuron, chlorthal, chlorthal-dimethyl, cinidon-ethyl, cinmethylin, cinosulfuron, cisanilide, clethodim, clodinafop, clodinafop-propargyl, clomazone, clomeprop, clopyralid, cloransulam, cloransulam-methyl, CMA, 4-CPB, CPMF, 4-CPP, CPPC, cresol, cumyluron, cyanamide, cyanazine, cycloate, cyclosulfamuron, cycloxydim, cyhalofop, cyhalofop-butyl, 2,4-D, 3,4-DA, daimuron, dalapon, dazomet, 2,4-DB, 3,4-DB, 2,4-DEB, desmedipham, dicamba, dichlobenil, ortho-dichlorobenzene, para-dichlorobenzene, dichlorprop, dichlorprop-P, diclofop, diclofop-methyl, diclosulam, difenzoquat, difenzoquat metilsulfate, diflufenican, diflufenzopyr, dimefuron, dimepiperate, dimethachlor, dimethametryn, dimethenamid, dimethenamid-P, dimethipin, dimethylarsinic acid, dinitramine, dinoterb, diphenamid, diquat, diquat dibromide, dithiopyr, diuron, DNOC, 3,4-DP, DSMA, EBEP, endothal, EPTC, esprocarb, ethalfluralin, ethametsulfuron, ethametsulfuron-methyl, ethofumesate, ethoxyfen, ethoxysulfuron, etobenzanid, fenoxaprop-P, fenoxaprop-P-ethyl, fentrazamide, ferrous sulfate, flamprop-M, flazasulfuron, florasulam, fluazifop, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl, flucarbazone, flucarbazone-sodium, flucetosulfuron, fluchloralin, flufenacet, flufenpyr, flufenpyr-ethyl, flumetsulam, flumiclorac, flumiclorac-pentyl, flumioxazin, fluometuron, fluoroglycofen, fluoroglycofen-ethyl, flupropanate, flupyrsulfuron, flupyrsulfuron-methyl-sodium, flurenol, fluridone, fluorochloridone, fluoroxypyr, flurtamone, fluthiacet, fluthiacet-methyl, fomesafen, foramsulfuron, fosamine, glufosinate, glufosinate-ammonium, glyphosate, halosulfuron, halosulfuron-methyl, haloxyfop, haloxyfop-P, HC-252, hexazinone, imazamethabenz, imazamethabenz-methyl, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, imazosulfuron, indanofan, iodomethane, iodosulfuron, iodosulfuron-methyl-sodium, ioxynil, isoproturon, isouron, isoxaben, isoxachlortole, isoxaflutole, karbutilate, lactofen, lenacil, linuron, MAA, MAMA, MCPA, MCPA-thioethyl, MCPB, mecoprop, mecoprop-P, mefenacet, mefluidide, mesosulfuron, mesosulfuron-methyl, mesotrione, metam, metamifop, metamitron, metazachlor, methabenzthiazuron, methylarsonic acid, methyldymron, methyl isothiocyanate, metobenzuron, metolachlor, S-metolachlor, metosulam, metoxuron, metribuzin, metsulfuron, metsulfuron-methyl, MK-66, molinate, monolinuron, MSMA, naproanilide, napropamide, naptalam, neburon, nicosulfuron, nonanoic acid, norflurazon, oleic acid (fatty acids), orbencarb, orthosulfamuron, oryzalin, oxadiargyl, oxadiazon, oxasulfuron, oxaziclomefone, oxyfluorfen, paraquat, paraquat dichloride, pebulate, pendimethalin, penoxsulam, pentachlorophenol, pentanochlor, pentoxazone, pethoxamid, petrolium oils, phenmedipham, phenmedipham-ethyl, picloram, picolinafen, pinoxaden, piperophos, potassium arsenite, potassium azide, pretilachlor, primisulfuron, primisulfuron-methyl, prodiamine, profluazol, profoxydim, prometon, prometryn, propachlor, propanil, propaquizafop, propazine, propham, propisochlor, propoxycarbazone, propoxycarbazone-sodium, propyzamide, prosulfocarb, prosulfuron, pyraclonil, pyraflufen, pyraflufen-ethyl, pyrazolynate, pyrazosulfuron, pyrazosulfuron-ethyl, pyrazoxyfen, pyribenzoxim, pyributicarb, pyridafol, pyridate, pyriftalid, pyriminobac, pyriminobac-methyl, pyrimisulfan, pyrithiobac, pyrithiobac-sodium, quinclorac, quinmerac, quinoclamine, quizalofop, quizalofop-P, rimsulfuron, sethoxydim, siduron, simazine, simetryn, SMA, sodium arsenite, sodium azide, sodium chlorate, sulcotrione, sulfentrazone, sulfometuron, sulfometuron-methyl, sulfosate, sulfosulfuron, sulfuric acid, tar oils, 2,3,6-TBA, TCA, TCA-sodium, tebuthiuron, tepraloxydim, terbacil, terbumeton, terbuthylazine, terbutryn, thenylchlor, thiazopyr, thifensulfuron, thifensulfuron-methyl, thiobencarb, tiocarbazil, topramezone, tralkoxydim, tri-allate, triasulfuron, triaziflam, tribenuron, tribenuron-methyl, tricamba, triclopyr, trietazine, trifloxysulfuron, trifloxysulfuron-sodium, trifluralin, triflusulfuron, triflusulfuron-methyl, trihydroxytriazine, tritosulfuron, [3-[2-chloro-4-fluoro-5-(methyl-6-trifluoromethyl-2,4-dioxo-2,3,4-tetrahydropyrimidin-3-yl)phenoxy]-2-pyridyloxy]acetic acid ethyl ester (CAS RN 353292-3-6), 4-[(4,5-dihydro-3-methoxy-4-methyl-5-oxo)-H-,2,4-triazolylcarbonyl-sulfamoyl]-5-methylthiophene-3-carboxylic acid (BAY636), BAY747 (CAS RN 33504-84-2), topramezone (CAS RN 2063-68-8), 4-hydroxy-3-[[2-[(2-methoxyethoxy)methyl]-6-(trifluoro-methyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.]oct-3-en-2-one (CAS RN 35200-68-5), and 4-hydroxy-3-[[2-(3-methoxypropyl)-6-(difluoromethyl)-3-pyridinyl]carbonyl]-bicyclo[3.2.]oct-3-en-2-one. Additionally, including herbicidal compounds of unspecified modes of action as described in CN101279950A, CN101279951A, DE10000600A1, DE10116399A1, DE102004054666A1, DE102005014638A1, DE102005014906A1, DE102007012168A1, DE102010042866A1, DE10204951A1, DE10234875A1, DE10234876A1, DE10256353A1, DE10256354A1, DE10256367A1, EP1157991A2, EP1238586A1, EP2147919A1, EP2160098A2, JP03968012B2, JP2001253874A, JP2002080454A, JP2002138075A, JP2002145707A, JP2002220389A, JP2003064059A, JP2003096059A, JP2004051628A, JP2004107228A, JP2005008583A, JP2005239675A, JP2005314407A, JP2006232824A, JP2006282552A, JP2007153847A, JP2007161701A, JP2007182404A, JP2008074840A, JP2008074841A, JP2008133207A, JP2008133218A, JP2008169121A, JP2009067739A, JP2009114128A, JP2009126792A, JP2009137851A, US20060111241A1, US20090036311A1, US20090054240A1, US20090215628A1, US20100099561A1, US20100152443A1, US20110105329A1, US20110201501A1, WO2001055066A2, WO2001056975A1, WO2001056979A1, WO2001090071A2, WO2001090080A1, WO2002002540A1, WO2002028182A1, WO2002040473A1, WO2002044173A2, WO2003000679A2, WO2003006422A1, WO2003013247A1, WO2003016308A1, WO2003020704A1, WO2003022051A1, WO2003022831A1, WO2003022843A1, WO2003029243A2, WO2003037085A1, WO2003037878A1, WO2003045878A2, WO2003050087A2, WO2003051823A1, WO2003051824A1, WO2003051846A2, WO2003076409A1, WO2003087067A1, WO2003090539A1, WO2003091217A1, WO2003093269A2, WO2003104206A2, WO2004002947A1, WO2004002981A2, WO2004011429A1, WO2004029060A1, WO2004035545A2, WO2004035563A1, WO2004035564A1, WO2004037787A1, WO2004067518A1, WO2004067527A1, WO2004077950A1, WO2005000824A1, WO2005007627A1, WO2005040152A1, WO2005047233A1, WO2005047281A1, WO2005061443A2, WO2005061464A1, WO2005068434A1, WO2005070889A1, WO2005089551A1, WO2005095335A1, WO2006006569A1, WO2006024820A1, WO2006029828A1, WO2006029829A1, WO2006037945A1, WO2006050803A1, WO2006090792A1, WO2006123088A2, WO2006125687A1, WO2006125688A1, WO2007003294A1, WO2007026834A1, WO2007071900A1, WO2007077201A1, WO2007077247A1, WO2007096576A1, WO2007119434A1, WO2007134984A1, WO2008009908A1, WO2008029084A1, WO2008059948A1, WO2008071918A1, WO2008074991A1, WO2008084073A1, WO2008100426A2, WO2008102908A1, WO2008152072A2, WO2008152073A2, WO2009000757A1, WO2009005297A2, WO2009035150A2, WO2009063180A1, WO2009068170A2, WO2009068171A2, WO2009086041A1, WO2009090401A2, WO2009090402A2, WO2009115788A1, WO2009116558A1, WO2009152995A1, WO2009158258A1, WO2010012649A1, WO2010012649A1, WO2010026989A1, WO2010034153A1, WO2010049270A1, WO2010049369A1, WO2010049405A1, WO2010049414A1, WO2010063422A1, WO2010069802A1, WO2010078906A2, WO2010078912A1, WO2010104217A1, WO2010108611A1, WO2010112826A3, WO2010116122A3, WO2010119906A1, WO2010130970A1, WO2011003776A2, WO2011035874A1, WO2011065451A1, all of which are incorporated herein by reference.

An agronomic field in need of plant control is treated by application of the composition directly to the surface of the growing plants, such as by a spray. For example, the method is applied to control weeds in a field of crop plants by spraying the field with the composition. The composition can be provided as a tank mix, a sequential treatment of components (generally the polynucleotide containing composition followed by the herbicide), or a simultaneous treatment or mixing of one or more of the components of the composition from separate containers. Treatment of the field can occur as often as needed to provide weed control and the components of the composition can be adjusted to target specific weed species or weed families through utilization of specific polynucleotides or polynucleotide compositions capable of selectively targeting the specific species or plant family to be controlled. The composition can be applied at effective use rates according to the time of application to the field, for example, preplant, at planting, post planting, post harvest. PPG oxidase inhibitor herbicides can be applied to a field at rates of 100 to 500 g ai/ha (active ingredient per hectare) or more. The polynucleotides of the composition can be applied at rates of 1 to 30 grams per acre depending on the number of trigger molecules needed for the scope of weeds in the field.

Crop plants in which weed control is needed include, but are not limited to, i) corn, soybean, cotton, canola, sugar beet, alfalfa, sugarcane, rice, and wheat; ii) vegetable plants including, but not limited to, tomato, sweet pepper, hot pepper, melon, watermelon, cucumber, eggplant, cauliflower, broccoli, lettuce, spinach, onion, peas, carrots, sweet corn, Chinese cabbage, leek, fennel, pumpkin, squash or gourd, radish, Brussels sprouts, tomatillo, garden beans, dry beans, or okra; iii) culinary plants including, but not limited to, basil, parsley, coffee, or tea; iv) fruit plants including, but not limited to, apple, pear, cherry, peach, plum, apricot, banana, plantain, table grape, wine grape, citrus, avocado, mango, or berry; v) a tree grown for ornamental or commercial use, including, but not limited to, a fruit or nut tree; or vi) an ornamental plant (e.g., an ornamental flowering plant or shrub or turf grass). The methods and compositions provided herein can also be applied to plants produced by a cutting, cloning, or grafting process (i.e., a plant not grown from a seed) including fruit trees and plants that include, but are not limited to, citrus, apples, avocados, tomatoes, eggplant, cucumber, melons, watermelons, and grapes, as well as various ornamental plants.

Pesticidal Mixtures

The polynucleotide compositions may also be used as mixtures with various agricultural chemicals and/or insecticides, miticides and fungicides, pesticidal and biopesticidal agents. Examples include but are not limited to azinphos-methyl, acephate, isoxathion, isofenphos, ethion, etrimfos, oxydemeton-methyl, oxydeprofos, quinalphos, chlorpyrifos, chlorpyrifos-methyl, chlorfenvinphos, cyanophos, dioxabenzofos, dichlorvos, disulfoton, dimethylvinphos, dimethoate, sulprofos, diazinon, thiometon, tetrachlorvinphos, temephos, tebupirimfos, terbufos, naled, vamidothion, pyraclofos, pyridafenthion, pirimiphos-methyl, fenitrothion, fenthion, phenthoate, flupyrazophos, prothiofos, propaphos, profenofos, phoxime, phosalone, phosmet, formothion, phorate, malathion, mecarbam, mesulfenfos, methamidophos, methidathion, parathion, methyl parathion, monocrotophos, trichlorphon, EPN, isazophos, isamidofos, cadusafos, diamidaphos, dichlofenthion, thionazin, fenamiphos, fosthiazate, fosthietan, phosphocarb, DSP, ethoprophos, alanycarb, aldicarb, isoprocarb, ethiofencarb, carbaryl, carbosulfan, xylylcarb, thiodicarb, pirimicarb, fenobucarb, furathiocarb, propoxur, bendiocarb, benfuracarb, methomyl, metolcarb, XMC, carbofuran, aldoxycarb, oxamyl, acrinathrin, allethrin, esfenvalerate, empenthrin, cycloprothrin, cyhalothrin, gamma-cyhalothrin, lambda-cyhalothrin, cyfluthrin, beta-cyfluthrin, cypermethrin, alpha-cypermethrin, zeta-cypermethrin, silafluofen, tetramethrin, tefluthrin, deltamethrin, tralomethrin, bifenthrin, phenothrin, fenvalerate, fenpropathrin, furamethrin, prallethrin, flucythrinate, fluvalinate, flubrocythrinate, permethrin, resmethrin, ethofenprox, cartap, thiocyclam, bensultap, acetamiprid, imidacloprid, clothianidin, dinotefuran, thiacloprid, thiamethoxam, nitenpyram, chlorfluazuron, diflubenzuron, teflubenzuron, triflumuron, novaluron, noviflumuron, bistrifluoron, fluazuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, chromafenozide, tebufenozide, halofenozide, methoxyfenozide, diofenolan, cyromazine, pyriproxyfen, buprofezin, methoprene, hydroprene, kinoprene, triazamate, endosulfan, chlorfenson, chlorobenzilate, dicofol, bromopropylate, acetoprole, fipronil, ethiprole, pyrethrin, rotenone, nicotine sulphate, BT (Bacillus Thuringiensis) agent, spinosad, abamectin, acequinocyl, amidoflumet, amitraz, etoxazole, chinomethionat, clofentezine, fenbutatin oxide, dienochlor, cyhexatin, spirodiclofen, spiromesifen, tetradifon, tebufenpyrad, binapacryl, bifenazate, pyridaben, pyrimidifen, fenazaquin, fenothiocarb, fenpyroximate, fluacrypyrim, fluazinam, flufenzin, hexythiazox, propargite, benzomate, polynactin complex, milbemectin, lufenuron, mecarbam, methiocarb, mevinphos, halfenprox, azadirachtin, diafenthiuron, indoxacarb, emamectin benzoate, potassium oleate, sodium oleate, chlorfenapyr, tolfenpyrad, pymetrozine, fenoxycarb, hydramethylnon, hydroxy propyl starch, pyridalyl, flufenerim, flubendiamide, flonicamid, metaflumizole, lepimectin, TPIC, albendazole, oxibendazole, oxfendazole, trichlamide, fensulfothion, fenbendazole, levamisole hydrochloride, morantel tartrate, dazomet, metam-sodium, triadimefon, hexaconazole, propiconazole, ipconazole, prochloraz, triflumizole, tebuconazole, epoxiconazole, difenoconazole, flusilazole, triadimenol, cyproconazole, metconazole, fluquinconazole, bitertanol, tetraconazole, triticonazole, flutriafol, penconazole, diniconazole, fenbuconazole, bromuconazole, imibenconazole, simeconazole, myclobutanil, hymexazole, imazalil, furametpyr, thifluzamide, etridiazole, oxpoconazole, oxpoconazole fumarate, pefurazoate, prothioconazole, pyrifenox, fenarimol, nuarimol, bupirimate, mepanipyrim, cyprodinil, pyrimethanil, metalaxyl, mefenoxam, oxadixyl, benalaxyl, thiophanate, thiophanate-methyl, benomyl, carbendazim, fuberidazole, thiabendazole, manzeb, propineb, zineb, metiram, maneb, ziram, thiuram, chlorothalonil, ethaboxam, oxycarboxin, carboxin, flutolanil, silthiofam, mepronil, dimethomorph, fenpropidin, fenpropimorph, spiroxamine, tridemorph, dodemorph, flumorph, azoxystrobin, kresoxim-methyl, metominostrobin, orysastrobin, fluoxastrobin, trifloxystrobin, dimoxystrobin, pyraclostrobin, picoxystrobin, iprodione, procymidone, vinclozolin, chlozolinate, flusulfamide, dazomet, methyl isothiocyanate, chloropicrin, methasulfocarb, hydroxyisoxazole, potassium hydroxyisoxazole, echlomezol, D-D, carbam, basic copper chloride, basic copper sulfate, copper nonylphenolsulfonate, oxine copper, DBEDC, anhydrous copper sulfate, copper sulfate pentahydrate, cupric hydroxide, inorganic sulfur, wettable sulfur, lime sulfur, zinc sulfate, fentin, sodium hydrogen carbonate, potassium hydrogen carbonate, sodium hypochlorite, silver, edifenphos, tolclofos-methyl, fosetyl, iprobenfos, dinocap, pyrazophos, carpropamid, fthalide, tricyclazole, pyroquilon, diclocymet, fenoxanil, kasugamycin, validamycin, polyoxins, blasticiden S, oxytetracycline, mildiomycin, streptomycin, rape seed oil, machine oil, benthiavalicarbisopropyl, iprovalicarb, propamocarb, diethofencarb, fluoroimide, fludioxanil, fenpiclonil, quinoxyfen, oxolinic acid, chlorothalonil, captan, folpet, probenazole, acibenzolar-S-methyl, tiadinil, cyflufenamid, fenhexamid, diflumetorim, metrafenone, picobenzamide, proquinazid, famoxadone, cyazofamid, fenamidone, zoxamide, boscalid, cymoxanil, dithianon, fluazinam, dichlofluanide, triforine, isoprothiolane, ferimzone, diclomezine, tecloftalam, pencycuron, chinomethionat, iminoctadine acetate, iminoctadine albesilate, ambam, polycarbamate, thiadiazine, chloroneb, nickel dimethyldithiocarbamate, guazatine, dodecylguanidine-acetate, quintozene, tolylfluanid, anilazine, nitrothalisopropyl, fenitropan, dimethirimol, benthiazole, harpin protein, flumetover, mandipropamide and penthiopyrad.

Polynucleotides

As used herein, the terms “DNA,” “DNA molecule,” and “DNA polynucleotide molecule” refer to a single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) molecule of genomic or synthetic origin, such as a polymer of deoxyribonucleotide bases or a DNA polynucleotide molecule. As used herein, the terms “DNA sequence,” “DNA nucleotide sequence” or “DNA polynucleotide sequence” refer to the nucleotide sequence of a DNA molecule. As used herein, the terms “RNA,” “RNA molecule,” “RNA polynucleotide molecule” refer to a single-stranded RNA (ssRNA) or double-stranded RNA (dsRNA) molecule of genomic or synthetic origin, such as a polymer of ribonucleotide bases that comprise single or double stranded regions. Unless otherwise stated, nucleotide sequences in the text of this specification are given, when read from left to right, in the 5′ to 3′ direction. The nomenclature used herein is that required by Title 37 of the United States Code of Federal Regulations § 1.822 and set forth in the tables in WIPO Standard ST.25 (1998), Appendix 2, Tables 1 and 3.

As used herein, “polynucleotide” refers to a DNA or RNA molecule containing multiple nucleotides and generally refers both to “oligonucleotides” (a polynucleotide molecule of typically 50 or fewer nucleotides in length) and polynucleotides of 51 or more nucleotides. Embodiments include compositions including oligonucleotides having a length of 18-25 nucleotides (18-mers, 19-mers, 20-mers, 21-mers, 22-mers, 23-mers, 24-mers, or 25-mers), for example, oligonucleotides of Table 3 (SEQ ID NOs:1382-2221) or fragments thereof or medium-length polynucleotides having a length of 26 or more nucleotides (polynucleotides of 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, about 65, about 70, about 75, about 80, about 85, about 90, about 95, about 100, about 110, about 120, about 130, about 140, about 150, about 160, about 170, about 180, about 190, about 200, about 210, about 220, about 230, about 240, about 250, about 260, about 270, about 280, about 290, or about 300 nucleotides), for example, oligonucleotides of Table 2 (SEQ ID NOs:72-1381) or fragments thereof or long polynucleotides having a length greater than about 300 nucleotides (for example, polynucleotides of between about 300 to about 400 nucleotides, between about 400 to about 500 nucleotides, between about 500 to about 600 nucleotides, between about 600 to about 700 nucleotides, between about 700 to about 800 nucleotides, between about 800 to about 900 nucleotides, between about 900 to about 1000 nucleotides, between about 300 to about 500 nucleotides, between about 300 to about 600 nucleotides, between about 300 to about 700 nucleotides, between about 300 to about 800 nucleotides, between about 300 to about 900 nucleotides, or about 1000 nucleotides in length, or even greater than about 1000 nucleotides in length, for example, up to the entire length of a target gene including coding or non-coding or both coding and non-coding portions of the target gene), for example, polynucleotides of Table 1 (SEQ ID NOs:1-71), wherein the selected polynucleotides or fragments thereof homologous or complementary to SEQ ID NOs:1-71 suppresses, represses or otherwise delay the expression of the target PPG oxidase gene. A target gene comprises any polynucleotide molecule in a plant cell or fragment thereof for which the modulation of the expression of the target gene is provided by the methods and compositions. Where a polynucleotide is double-stranded, its length can be similarly described in terms of base pairs. Oligonucleotides and polynucleotides can be made that are essentially identical or essentially complementary to adjacent genetic elements of a gene, for example, spanning the junction region of an intron and exon, the junction region of a promoter and a transcribed region, the junction region of a 5′ leader and a coding sequence, the junction of a 3′ untranslated region and a coding sequence.

Polynucleotide compositions used in the various embodiments include compositions including oligonucleotides or polynucleotides or a mixture of both, including RNA or DNA or RNA/DNA hybrids or chemically modified oligonucleotides or polynucleotides or a mixture thereof. In some embodiments, the polynucleotide may be a combination of ribonucleotides and deoxyribonucleotides, for example, synthetic polynucleotides consisting mainly of ribonucleotides but with one or more terminal deoxyribonucleotides or synthetic polynucleotides consisting mainly of deoxyribonucleotides but with one or more terminal dideoxyribonucleotides. In some embodiments, the polynucleotide includes non-canonical nucleotides such as inosine, thiouridine, or pseudouridine. In some embodiments, the polynucleotide includes chemically modified nucleotides. Examples of chemically modified oligonucleotides or polynucleotides are well known in the art; see, for example, US Patent Publication 20110171287, US Patent Publication 20110171176, US Patent Publication 20110152353, US Patent Publication, 20110152346, and US Patent Publication 20110160082, each of which is herein incorporated by reference in its entirety. For example, including but not limited to the naturally occurring phosphodiester backbone of an oligonucleotide or polynucleotide can be partially or completely modified with phosphorothioate, phosphorodithioate, or methylphosphonate internucleotide linkage modifications, modified nucleoside bases or modified sugars can be used in oligonucleotide or polynucleotide synthesis, and oligonucleotides or polynucleotides can be labeled with a fluorescent moiety (for example, fluorescein or rhodamine) or other label (for example, biotin).

The polynucleotides can be single- or double-stranded RNA or single- or double-stranded DNA or double-stranded DNA/RNA hybrids or modified analogues thereof, and can be of oligonucleotide lengths or longer. In more specific embodiments the polynucleotides that provide single-stranded RNA in the plant cell are selected from the group consisting of (a) a single-stranded RNA molecule (ssRNA), (b) a single-stranded RNA molecule that self-hybridizes to form a double-stranded RNA molecule, (c) a double-stranded RNA molecule (dsRNA), (d) a single-stranded DNA molecule (ssDNA), (e) a single-stranded DNA molecule that self-hybridizes to form a double-stranded DNA molecule, (f) a single-stranded DNA molecule including a modified Pol III gene that is transcribed to an RNA molecule, (g) a double-stranded DNA molecule (dsDNA), (h) a double-stranded DNA molecule including a modified Pol III gene that is transcribed to an RNA molecule, and (i) a double-stranded, hybridized RNA/DNA molecule, or combinations thereof. In some embodiments, these polynucleotides include chemically modified nucleotides or non-canonical nucleotides. In some embodiments, the oligonucleotides may be blunt-ended or may comprise a 3′ overhang of from 1-5 nucleotides of at least one or both of the strands. Other configurations of the oligonucleotide are known in the field and are contemplated herein. In embodiments of the method, the polynucleotides include double-stranded DNA formed by intramolecular hybridization, double-stranded DNA formed by intermolecular hybridization, double-stranded RNA formed by intramolecular hybridization, or double-stranded RNA formed by intermolecular hybridization. In one embodiment, the polynucleotides include single-stranded DNA or single-stranded RNA that self-hybridizes to form a hairpin structure having an at least partially double-stranded structure including at least one segment that will hybridize to RNA transcribed from the gene targeted for suppression. Not intending to be bound by any mechanism, it is believed that such polynucleotides are or will produce single-stranded RNA with at least one segment that will hybridize to RNA transcribed from the gene targeted for suppression. In certain other embodiments, the polynucleotides further include a promoter, generally a promoter functional in a plant, for example, a pol II promoter, a pol III promoter, a pol IV promoter, or a pol V promoter.

The term “gene” refers to components that comprise chromosomal DNA, plasmid DNA, cDNA, intron and exon DNA, artificial DNA polynucleotide, or other DNA that encodes a peptide, polypeptide, protein, or RNA transcript molecule, and the genetic elements flanking the coding sequence that are involved in the regulation of expression, such as promoter regions, 5′ leader regions, 3′ untranslated region that may exist as native genes or transgenes in a plant genome. The gene or a fragment thereof is isolated and subjected to polynucleotide sequencing methods that determines the order of the nucleotides that comprise the gene. Any of the components of the gene are potential targets for a trigger oligonucleotide and polynucleotides.

The trigger polynucleotide molecules are designed to modulate expression by inducing regulation or suppression of an endogenous PPG oxidase gene in a plant and are designed to have a nucleotide sequence essentially identical or essentially complementary to the nucleotide sequence of an endogenous PPG oxidase gene of a plant or to the sequence of RNA transcribed from an endogenous PPG oxidase gene of a plant, including a transgene in a plant that provides for a herbicide resistant PPG oxidase enzyme, which can be a coding sequence or a non-coding sequence. Effective molecules that modulate expression are referred to as “a trigger molecule, or trigger polynucleotides.” By “essentially identical” or “essentially complementary” is meant that the trigger polynucleotides (or at least one strand of a double-stranded polynucleotide or portion thereof, or a portion of a single strand polynucleotide) are designed to hybridize to the endogenous gene noncoding sequence or to RNA transcribed (known as messenger RNA or an RNA transcript) from the endogenous gene to effect regulation or suppression of expression of the endogenous gene. Trigger molecules are identified by “tiling” the gene targets with partially overlapping probes or non-overlapping probes of antisense or sense polynucleotides that are essentially identical or essentially complementary to the nucleotide sequence of an endogenous gene. Multiple target sequences can be aligned and sequence regions with homology in common, according to the methods, are identified as potential trigger molecules for the multiple targets. Multiple trigger molecules of various lengths, for example 18-25 nucleotides, 26-50 nucleotides, 51-100 nucleotides, 101-200 nucleotides, 201-300 nucleotides or more can be pooled into a few treatments in order to investigate polynucleotide molecules that cover a portion of a gene sequence (for example, a portion of a coding versus a portion of a noncoding region, or a 5′ versus a 3′ portion of a gene) or an entire gene sequence including coding and noncoding regions of a target gene. Polynucleotide molecules of the pooled trigger molecules can be divided into smaller pools or single molecules in order to identify trigger molecules that provide the desired effect.

The target gene RNA and DNA polynucleotide molecules (Table 1, SEQ ID NOs:1-71) are sequenced by any number of available methods and equipment. Some of the sequencing technologies are available commercially, such as the sequencing-by-hybridization platform from Affymetrix Inc. (Sunnyvale, Calif.) and the sequencing-by-synthesis platforms from 454 Life Sciences (Bradford, Conn.), Illumina/Solexa (Hayward, Calif.) and Helicos Biosciences (Cambridge, Mass.), and the sequencing-by-ligation platform from Applied Biosystems (Foster City, Calif.), as described below. In addition to the single molecule sequencing performed using sequencing-by-synthesis of Helicos Biosciences, other single molecule sequencing technologies are encompassed by the method and include the SMRT™ technology of Pacific Biosciences, the Ion Torrent™ technology, and nanopore sequencing being developed for example, by Oxford Nanopore Technologies. A PPG oxidase target gene comprising DNA or RNA can be isolated using primers or probes essentially complementary or essentially homologous to SEQ ID NOs:1-71 or a fragment thereof. A polymerase chain reaction (PCR) gene fragment can be produced using primers essentially complementary or essentially homologous to SEQ IDs NO:1-71 or a fragment thereof that is useful to isolate a PPG oxidase gene from a plant genome. SEQ ID NOs: 1-71 or fragments thereof can be used in various sequence capture technologies to isolate additional target gene sequences, for example, including but not limited to Roche NimbleGen® (Madison, Wis.) and Streptavdin-coupled Dynabeads® (Life Technologies, Grand Island, N.Y.) and US Patent Publication 20110015284, herein incorporated by reference in its entirety.

Embodiments of functional single-stranded polynucleotides have sequence complementarity that need not be 100 percent, but is at least sufficient to permit hybridization to RNA transcribed from the target gene or DNA of the target gene to form a duplex to permit a gene silencing mechanism. Thus, in embodiments, a polynucleotide fragment is designed to be essentially identical to, or essentially complementary to, a sequence of 18 or more contiguous nucleotides in either the target PPG oxidase gene sequence or messenger RNA transcribed from the target gene. By “essentially identical” is meant having 100 percent sequence identity or at least about 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 percent sequence identity when compared to the sequence of 18 or more contiguous nucleotides in either the target gene or RNA transcribed from the target gene; by “essentially complementary” is meant having 100 percent sequence complementarity or at least about 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 percent sequence complementarity when compared to the sequence of 18 or more contiguous nucleotides in either the target gene or RNA transcribed from the target gene. In some embodiments, polynucleotide molecules are designed to have 100 percent sequence identity with or complementarity to one allele or one family member of a given target gene (coding or non-coding sequence of a gene); in other embodiments, the polynucleotide molecules are designed to have 100 percent sequence identity with or complementarity to multiple alleles or family members of a given target gene. The trigger polynucleotide sequences in the sequence listing SEQ ID NOs: 1-2221 or table 1, 2 or 3 may be complementary or homologous to a portion of the PPG oxidase target gene sequence.

In certain embodiments, the polynucleotides used in the compositions that are essentially identical or essentially complementary to the target gene or transcript will comprise the predominant nucleic acid in the composition. Thus in certain embodiments, the polynucleotides that are essentially identical or essentially complementary to the target gene or transcript will comprise at least about 50%, 75%, 95%, 98% or 100% of the nucleic acids provided in the composition by either mass or molar concentration. However, in certain embodiments, the polynucleotides that are essentially identical or essentially complementary to the target gene or transcript can comprise at least about 1% to about 50%, about 10% to about 50%, about 20% to about 50%, or about 30% to about 50% of the nucleic acids provided in the composition by either mass or molar concentration. Also provided are compositions where the polynucleotides that are essentially identical or essentially complementary to the target gene or transcript can comprise at least about 1% to 100%, about 10% to 100%, about 20% to about 100%, about 30% to about 50%, or about 50% to a 100% of the nucleic acids provided in the composition by either mass or molar concentration.

“Identity” refers to the degree of similarity between two polynucleic acid or protein sequences. An alignment of the two sequences is performed by a suitable computer program. A widely used and accepted computer program for performing sequence alignments is CLUSTALW v1.6 (Thompson et al., Nucl. Acids Res., 22: 4673-4680, 1994). The number of matching bases or amino acids is divided by the total number of bases or amino acids, and multiplied by 100 to obtain a percent identity. For example, if two 580 base pair sequences had 145 matched bases, they would be 25 percent identical. If the two compared sequences are of different lengths, the number of matches is divided by the shorter of the two lengths. For example, if there are 100 matched amino acids between a 200 and a 400 amino acid protein, they are 50 percent identical with respect to the shorter sequence. If the shorter sequence is less than 150 bases or 50 amino acids in length, the number of matches are divided by 150 (for nucleic acid bases) or 50 (for amino acids), and multiplied by 100 to obtain a percent identity.

Trigger molecules for specific gene family members can be identified from coding and/or non-coding sequences of gene families of a plant or multiple plants, by aligning and selecting 200-300 polynucleotide fragments from the least homologous regions amongst the aligned sequences and evaluated using topically applied polynucleotides (as sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA) to determine their relative effectiveness in inducing the herbicidal phenotype. The effective segments are further subdivided into 50-60 polynucleotide fragments, prioritized by least homology, and reevaluated using topically applied polynucleotides. The effective 50-60 polynucleotide fragments are subdivided into 19-30 polynucleotide fragments, prioritized by least homology, and again evaluated for induction of the yield/quality phenotype. Once relative effectiveness is determined, the fragments are utilized singly, or again evaluated in combination with one or more other fragments to determine the trigger composition or mixture of trigger polynucleotides for providing the yield/quality phenotype.

Trigger molecules for broad activity can be identified from coding and/or non-coding sequences of gene families of a plant or multiple plants, by aligning and selecting 200-300 polynucleotide fragments from the most homologous regions amongst the aligned sequences and evaluated using topically applied polynucleotides (as sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA) to determine their relative effectiveness in inducing the yield/quality phenotype. The effective segments are subdivided into 50-60 polynucleotide fragments, prioritized by most homology, and reevaluated using topically applied polynucleotides. The effective 50-60 polynucleotide fragments are subdivided into 19-30 polynucleotide fragments, prioritized by most homology, and again evaluated for induction of the yield/quality phenotype. Once relative effectiveness is determined, the fragments may be utilized singly, or in combination with one or more other fragments to determine the trigger composition or mixture of trigger polynucleotides for providing the yield/quality phenotype.

Methods of making polynucleotides are well known in the art. Chemical synthesis, in vivo synthesis and in vitro synthesis methods and compositions are known in the art and include various viral elements, microbial cells, modified polymerases, and modified nucleotides. Commercial preparation of oligonucleotides often provides two deoxyribonucleotides on the 3′ end of the sense strand. Long polynucleotide molecules can be synthesized from commercially available kits, for example, kits from Applied Biosystems/Ambion (Austin, Tex.) have DNA ligated on the 5′ end in a microbial expression cassette that includes a bacterial T7 polymerase promoter that makes RNA strands that can be assembled into a dsRNA and kits provided by various manufacturers that include T7 RiboMax Express (Promega, Madison, Wis.), AmpliScribe T7-Flash (Epicentre, Madison, Wis.), and TranscriptAid T7 High Yield (Fermentas, Glen Burnie, Md.). dsRNA molecules can be produced from microbial expression cassettes in bacterial cells (Ongvarrasopone et al., ScienceAsia, 33:35-39; Yin, Appl. Microbiol. Biotechnol, 84:323-333, 2009; Liu et al., BMC Biotechnology, 10:85, 2010) that have regulated or deficient RNase III enzyme activity or the use of various viral vectors to produce sufficient quantities of dsRNA. PPG oxidase gene fragments are inserted into the microbial expression cassettes in a position in which the fragments are expressed to produce ssRNA or dsRNA useful in the methods described herein to regulate expression on a target PPG oxidase gene. Long polynucleotide molecules can also be assembled from multiple RNA or DNA fragments. In some embodiments design parameters such as Reynolds score (Reynolds et al., Nature Biotechnology, 22:326-330 (2004), Tuschl rules (Pei and Tuschl, Nature Methods, 3(9): 670-676, 2006), i-score (Nucleic Acids Res, 35:e123, 2007), i-Score Designer tool and associated algorithms (Nucleic Acids Res, 32:936-948, 2004; Biochem Biophys Res Commun, 316:1050-1058, 2004; Nucleic Acids Res, 32:893-901, 2004; Cell Cycle, 3: 790-5, 2004; Nat Biotechnol, 23:995-1001, 2005; Nucleic Acids Res, 35:e27, 2007; BMC Bioinformatics, 7:520, 2006; Nucleic Acids Res, 35:e123, 2007, Nat Biotechnol, 22:326-330, 2004) are known in the art and may be used in selecting polynucleotide sequences effective in gene silencing. In some embodiments, the sequence of a polynucleotide is screened against the genomic DNA of the intended plant to minimize unintentional silencing of other genes.

The trigger polynucleotide and oligonucleotide molecule compositions are useful in compositions, such as liquids that comprise these polynucleotide molecules, at low concentrations, alone or in combination with other components, for example one or more herbicide molecules, either in the same solution or in separately applied liquids that also provide a transfer agent. While there is no upper limit on the concentrations and dosages of polynucleotide molecules that can useful in the methods, lower effective concentrations and dosages will generally be sought for efficiency. The concentrations can be adjusted in consideration of the volume of spray or treatment applied to plant leaves or other plant part surfaces, such as flower petals, stems, tubers, fruit, anthers, pollen, or seed. In one embodiment, a useful treatment for herbaceous plants using 25-mer oligonucleotide molecules is about 1 nanomole (nmol) of oligonucleotide molecules per plant, for example, from about 0.05 to 1 nmol per plant. Other embodiments for herbaceous plants include useful ranges of about 0.05 to about 100 nmol, or about 0.1 to about 20 nmol, or about 1 nmol to about 10 nmol of polynucleotides per plant. Very large plants, trees, or vines may require correspondingly larger amounts of polynucleotides. When using long dsRNA molecules that can be processed into multiple oligonucleotides, lower concentrations can be used. To illustrate certain embodiments, the factor 1×, when applied to oligonucleotide molecules is arbitrarily used to denote a treatment of 0.8 nmol of polynucleotide molecule per plant; 10×, 8 nmol of polynucleotide molecule per plant; and 100×, 80 nmol of polynucleotide molecule per plant.

The polynucleotide compositions are useful in compositions, such as liquids that comprise polynucleotide molecules, alone or in combination with other components either in the same liquid or in separately applied liquids that provide a transfer agent. As used herein, a transfer agent is an agent that, when combined with a polynucleotide in a composition that is topically applied to a target plant surface, enables the polynucleotide to enter a plant cell. In certain embodiments, a transfer agent is an agent that conditions the surface of plant tissue, e.g., leaves, stems, roots, flowers, or fruits, to permeation by the polynucleotide molecules into plant cells. The transfer of polynucleotides into plant cells can be facilitated by the prior or contemporaneous application of a polynucleotide-transferring agent to the plant tissue. In some embodiments, the transferring agent is applied subsequent to the application of the polynucleotide composition. The polynucleotide transfer agent enables a pathway for polynucleotides through cuticle wax barriers, stomata and/or cell wall or membrane barriers into plant cells. Suitable transfer agents to facilitate transfer of the polynucleotide into a plant cell include agents that increase permeability of the exterior of the plant or that increase permeability of plant cells to oligonucleotides or polynucleotides. Such agents to facilitate transfer of the composition into a plant cell include a chemical agent, or a physical agent, or combinations thereof. Chemical agents for conditioning or transfer include (a) surfactants, (b) an organic solvent or an aqueous solution or aqueous mixtures of organic solvents, (c) oxidizing agents, (d) acids, (e) bases, (f) oils, (g) enzymes, or combinations thereof. Embodiments of the method can optionally include an incubation step, a neutralization step (e.g., to neutralize an acid, base, or oxidizing agent, or to inactivate an enzyme), a rinsing step, or combinations thereof. Embodiments of agents or treatments for conditioning of a plant to permeation by polynucleotides include emulsions, reverse emulsions, liposomes, and other micellar-like compositions. Embodiments of agents or treatments for conditioning of a plant to permeation by polynucleotides include counter-ions or other molecules that are known to associate with nucleic acid molecules, e.g., inorganic ammonium ions, alkyl ammonium ions, lithium ions, polyamines such as spermine, spermidine, or putrescine, and other cations. Organic solvents useful in conditioning a plant to permeation by polynucleotides include DMSO, DMF, pyridine, N-pyrrolidine, hexamethylphosphoramide, acetonitrile, dioxane, polypropylene glycol, other solvents miscible with water or that will dissolve phosphonucleotides in non-aqueous systems (such as is used in synthetic reactions). Naturally derived or synthetic oils with or without surfactants or emulsifiers can be used, e.g., plant-sourced oils, crop oils (such as those listed in the 9^(th) Compendium of Herbicide Adjuvants, publicly available on the worldwide web (internet) at herbicide.adjuvants.com) can be used, e.g., paraffinic oils, polyol fatty acid esters, or oils with short-chain molecules modified with amides or polyamines such as polyethyleneimine or N-pyrrolidine. Transfer agents include, but are not limited to, organosilicone preparations.

Ligands can be tethered to a polynucleotide, for example, a dsRNA, ssRNA, dsDNA or ssDNA. Ligands in general can include modifiers, e.g., for enhancing uptake; diagnostic compounds or reporter groups, e.g., for monitoring distribution; cross-linking agents; nuclease-resistance conferring moieties; and natural or unusual nucleobases. General examples include lipophiles, lipids (e.g., cholesterol), a bile acid, or a fatty acid (e.g., lithocholic-oleyl, lauroyl, docosnyl, stearoyl, palmitoyl, myristoyl oleoyl, linoleoyl), steroids (e.g., uvaol, hecigenin, diosgenin), terpenes (e.g., triterpenes, e.g., sarsasapogenin, Friedelin, epifriedelanol derivatized lithocholic acid), vitamins (e.g., folic acid, vitamin A, biotin, pyridoxal), carbohydrates, proteins, protein binding agents, integrin targeting molecules, polycationics, peptides, polyamines, and peptide mimics. The ligand may also be a recombinant or synthetic molecule, such as a synthetic polymer, e.g., polyethylene glycol (PEG), PEG-40K, PEG-20K and PEG-5K. Other examples of ligands include lipophilic molecules, e.g., cholesterol, cholic acid, adamantane acetic acid, 1-pyrene butyric acid, dihydrotestosterone, glycerol (e.g., esters and ethers thereof, e.g., C₁₀, C₁₁, C₁₂, C₁₃, C₁₄, C₁₅, C₁₆, C₁₇, C₁₈, C₁₉, or C₂₀ alkyl; e.g., lauroyl, docosnyl, stearoyl, oleoyl, linoleoyl 1,3-bis-O(hexadecyl)glycerol, 1,3-bis-O(octaadecyl)glycerol), geranyloxyhexyl group, hexadecylglycerol, borneol, menthol, 1,3-propanediol, heptadecyl group, palmitic acid, myristic acid, O3-(oleoyl)lithocholic acid, O3-(oleoyl)cholenic acid, dodecanoyl, lithocholyl, 5.beta.-cholanyl, N,N-distearyl-lithocholamide, 1,2-di-O-stearoylglyceride, dimethoxytrityl, or phenoxazine) and PEG (e.g., PEG-5K, PEG-20K, PEG-40K). Preferred lipophilic moieties include lipid, cholesterols, oleyl, retinyl, or cholesteryl residues.

Conjugating a ligand to a dsRNA can enhance its cellular absorption, lipophilic compounds that have been conjugated to oligonucleotides include 1-pyrene butyric acid, 1,3-bis-O-(hexadecyl)glycerol, and menthol. One example of a ligand for receptor-mediated endocytosis is folic acid. Folic acid enters the cell by folate-receptor-radiated endocytosis. dsRNA compounds bearing folic acid would be efficiently transported into the cell via the folate-receptor-mediated endocytosis. Other ligands that have been conjugated to oligonucleotides include polyethylene glycols, carbohydrate clusters, cross-linking agents, porphyrin conjugates, delivery peptides and lipids such as cholesterol. In certain instances, conjugation of a cationic ligand to oligonucleotides results in improved resistance to nucleases. Representative examples of cationic ligands are propylammonium and dimethylpropylammonium. Interestingly, antisense oligonucleotides were reported to retain their high binding affinity to mRNA when the cationic ligand was dispersed, throughout the oligonucleotide. See Manoharan, Antisense Nucleic Acid Drug Dev., 12(2):103-128 (2002) and references therein.

A biologic delivery can be accomplished by a variety of methods including, without limitation, (1) loading liposomes with a dsRNA acid molecule provided herein and (2) complexing a dsRNA molecule with lipids or liposomes to form nucleic acid-lipid or nucleic acid-liposome complexes. The liposome can be composed of cationic and neutral lipids commonly used to transfect cells in vitro. Cationic lipids can complex (e.g., charge-associate) with negatively charged, nucleic acids to form liposomes. Examples of cationic liposomes include, without limitation, LIPOFECTIN® (Invitrogen/Life Technologies, Carlsbad, Calif., a 1:1 (w.w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE)), LIPOFECTAMINE® *Invitrogen/Life Technologies, Carlsbad, Calif., a proprietary cationic liposome formulation), LIPOFECTACE® (Invitrogen/Life Technologies, Carlsbad, Calif., a liposome formulation of dimethyldioctadecyloammonium bromide (DDAB) and DOPE), and DOTAP. Procedures for forming liposomes are well known in the art. Liposome compositions can be formed, for example, from phosphatidylcholine, dimyristoyl phosphatidylcholine, dipalmitoyl phosphatidylcholine, dimyristoyl phosphatidyl glycerol, dioleoyl phosphatidylethanolamine or liposomes comprising dihydrosphingomyelin (DHSM). Numerous lipophilic agents are commercially available, including LIPOFECTIN® (Invitrogen/Life Technologies, Carlsbad, Calif.) and EFFECTENE™ (Qiagen, Valencia, Calif., a proprietary non-liposomal lipid formulation). In addition, systemic delivery methods can be optimized using commercially available cationic lipids such as DDAB or DOTAP, each of which can be mixed with a neutral lipid such as DOPE or cholesterol. In some cases, liposomes such as those described by Templeton et al., Nature Biotechnology, 15:647-652 (1997) can be used. In other embodiments, polycations such as polyethyleneimine can be used to achieve delivery in vivo and ex vivo (Boletta et al., J. Am Soc. Nephrol., 7:1728, 1996). Additional information regarding the use of liposomes to deliver nucleic acids can be found in U.S. Pat. No. 6,271,359, PCT Publication WO 96/40964, and Morrissey, D. et al., 2005, Nature Biotechnol. 23(8):1002-7.

In certain embodiments, an organosilicone preparation that is commercially available as Silwet® L-77 surfactant having CAS Number 27306-78-1 and EPA Number: CAL.REG.NO. 5905-50073-AA, and currently available from Momentive Performance Materials, Albany, N.Y., can be used to prepare a polynucleotide composition. In certain embodiments where a Silwet L-77 organosilicone preparation is used as a pre-spray treatment of plant leaves or other plant surfaces, freshly made concentrations in the range of about 0.015 to about 2 percent by weight (wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) are efficacious in preparing a leaf or other plant surface for transfer of polynucleotide molecules into plant cells from a topical application on the surface. In certain embodiments of the methods and compositions provided herein, a composition that comprises a polynucleotide molecule and an organosilicone preparation comprising Silwet L-77 in the range of about 0.015 to about 2 percent by weight (wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) is used or provided.

In certain embodiments, any of the commercially available organosilicone preparations provided such as the following Breakthru S 321, Breakthru S 200 Cat #67674-67-3, Breakthru OE 441 Cat #68937-55-3, Breakthru S 278 Cat #27306-78-1, Breakthru S 243, Breakthru S 233 Cat #134180-76-0, available from manufacturer Evonik Goldschmidt (Germany), Silwet® HS 429, Silwet® HS 312, Silwet® HS 508, Silwet® HS 604 (Momentive Performance Materials, Albany, N.Y.) can be used as transfer agents in a polynucleotide composition. In certain embodiments where an organosilicone preparation is used as a pre-spray treatment of plant leaves or other surfaces, freshly made concentrations in the range of about 0.015 to about 2 percent by weight (wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) are efficacious in preparing a leaf or other plant surface for transfer of polynucleotide molecules into plant cells from a topical application on the surface. In certain embodiments of the methods and compositions provided herein, a composition that comprises a polynucleotide molecule and an organosilicone preparation in the range of about 0.015 to about 2 percent by weight (wt percent) (e. g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) is used or provided.

Organosilicone preparations used in the methods and compositions provided herein can comprise one or more effective organosilicone compounds. As used herein, the phrase “effective organosilicone compound” is used to describe any organosilicone compound that is found in an organosilicone preparation that enables a polynucleotide to enter a plant cell. In certain embodiments, an effective organosilicone compound can enable a polynucleotide to enter a plant cell in a manner permitting a polynucleotide mediated suppression of a target gene expression in the plant cell. In general, effective organosilicone compounds include, but are not limited to, compounds that can comprise: i) a trisiloxane head group that is covalently linked to ii) an alkyl linker including, but not limited to, an n-propyl linker, that is covalently linked to iii) a poly glycol chain, that is covalently linked to iv) a terminal group. Trisiloxane head groups of such effective organosilicone compounds include, but are not limited to, heptamethyltrisiloxane. Alkyl linkers can include, but are not limited to, an n-propyl linker. Poly glycol chains include, but are not limited to, polyethylene glycol or polypropylene glycol. Poly glycol chains can comprise a mixture that provides an average chain length “n” of about “7.5.” In certain embodiments, the average chain length “n” can vary from about 5 to about 14. Terminal groups can include, but are not limited to, alkyl groups such as a methyl group. Effective organosilicone compounds are believed to include, but are not limited to, trisiloxane ethoxylate surfactants or polyalkylene oxide modified heptamethyl trisiloxane.

In certain embodiments, an organosilicone preparation that comprises an organosilicone compound comprising a trisiloxane head group is used in the methods and compositions provided herein. In certain embodiments, an organosilicone preparation that comprises an organosilicone compound comprising a heptamethyltrisiloxane head group is used in the methods and compositions provided herein. In certain embodiments, an organosilicone composition that comprises Compound I is used in the methods and compositions provided herein. In certain embodiments, an organosilicone composition that comprises Compound I is used in the methods and compositions provided herein. In certain embodiments of the methods and compositions provided herein, a composition that comprises a polynucleotide molecule and one or more effective organosilicone compounds in the range of about 0.015 to about 2 percent by weight (wt percent) (e.g., about 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04, 0.045, 0.05, 0.055, 0.06, 0.065, 0.07, 0.075, 0.08, 0.085, 0.09, 0.095, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.5 wt percent) is used or provided.

Compositions include but are not limited components that are one or more polynucleotides essentially identical to, or essentially complementary to, a PPG oxidase gene sequence (promoter, intron, exon, 5′ untranslated region, 3′ untranslated region), a transfer agent that provides for the polynucleotide to enter a plant cell, a herbicide that complements the action of the polynucleotide, one or more additional herbicides that further enhance the herbicide activity of the composition or provide an additional mode of action different from the complementing herbicide, various salts and stabilizing agents that enhance the utility of the composition as an admixture of the components of the composition.

The methods include one or more applications of a polynucleotide composition and one or more applications of a permeability-enhancing agent for conditioning of a plant to permeation by polynucleotides. When the agent for conditioning to permeation is an organosilicone composition or compound contained therein, embodiments of the polynucleotide molecules are double-stranded RNA oligonucleotides, single-stranded RNA oligonucleotides, double-stranded RNA polynucleotides, single-stranded RNA polynucleotides, double-stranded DNA oligonucleotides, single-stranded DNA oligonucleotides, double-stranded DNA polynucleotides, single-stranded DNA polynucleotides, chemically modified RNA or DNA oligonucleotides or polynucleotides or mixtures thereof.

Compositions and methods are useful for modulating the expression of an endogenous PPG oxidase gene (for example, U.S. Pat. Nos. 7,838,263 and 6,084,155) or transgenic PPG oxidase gene (U.S. Pat. Nos. 7,842,856 and 7,485,777; US Patent Publ. 20070050863) in a plant cell. In various embodiments, a PPG oxidase gene includes coding (protein-coding or translatable) sequence, non-coding (non-translatable) sequence, or both coding and non-coding sequence. Compositions can include polynucleotides and oligonucleotides designed to target multiple genes, or multiple segments of one or more genes. The target gene can include multiple consecutive segments of a target gene, multiple non-consecutive segments of a target gene, multiple alleles of a target gene, or multiple target genes from one or more species.

A method is provided for modulating expression of a PPG oxidase gene in a plant including (a) conditioning of a plant to permeation by polynucleotides and (b) treatment of the plant with the polynucleotide molecules, wherein the polynucleotide molecules include at least one segment of 18 or more contiguous nucleotides cloned from or otherwise identified from the target PPG oxidase gene in either anti-sense or sense orientation, whereby the polynucleotide molecules permeate the interior of the plant and induce modulation of the target gene. The conditioning and polynucleotide application can be performed separately or in a single step. When the conditioning and polynucleotide application are performed in separate steps, the conditioning can precede or can follow the polynucleotide application within minutes, hours, or days. In some embodiments more than one conditioning step or more than one polynucleotide molecule application can be performed on the same plant. In embodiments of the method, the segment can be cloned or identified from (a) coding (protein-encoding), (b) non-coding (promoter and other gene related molecules), or (c) both coding and non-coding parts of the target gene. Non-coding parts include DNA, such as promoter regions or the RNA transcribed by the DNA that provide RNA regulatory molecules, including but not limited to: introns, 5′ or 3′ untranslated regions, and microRNAs (miRNA), trans-acting siRNAs, natural anti-sense siRNAs, and other small RNAs with regulatory function or RNAs having structural or enzymatic function including but not limited to: ribozymes, ribosomal RNAs, t-RNAs, aptamers, and riboswitches.

All publications, patents and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.

The following examples are included to demonstrate examples of certain preferred embodiments. It should be appreciated by those of skill in the art that the techniques disclosed in the examples that follow represent approaches the inventors have found function well in the practice of the invention, and thus can be considered to constitute examples of preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments that are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

EXAMPLES Example 1. Polynucleotides Related to the PPG Oxidase Gene Sequences

The target PPG oxidase polynucleotide molecule naturally occurs in the genome of Amaranthus albus, Amaranthus graecizans, Amaranthus hybridus, Amaranthus lividus, Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus, Amaranthus thunbergii, Amaranthus viridis, Ambrosia trifida, Chenopodium album, Commelina diffusa, Conyza canadensis, Digitaria sanguinalis, Euphorbia heterophylla, Kochia scoparia, or Lolium multiflorum and include molecules related to the expression of a polypeptide identified as a PPG oxidase, that include regulatory molecules, cDNAs comprising coding and noncoding regions of a PPG oxidase gene and fragments thereof as shown in Table 1.

Polynucleotide molecules were extracted from these plant species by methods standard in the field, for example, total RNA was extracted using Trizol Reagent (Invitrogen Corp, Carlsbad, Calif. Cat. No. 15596-018), following the manufacturer's protocol or modifications thereof by those skilled in the art of polynucleotide extraction that may enhance recovery or purity of the extracted RNA. Briefly, start with 1 gram of ground plant tissue for extraction. Prealiquot 10 milliliters (mL) Trizol reagent to 15 mL conical tubes. Add ground powder to tubes and shake to homogenize. Incubate the homogenized samples for 5 minutes (min) at room temperature (RT) and then add 3 mL of chloroform. Shake tubes vigorously by hand for 15-30 seconds (sec) and incubate at RT for 3 min. Centrifuge the tubes at 7,000 revolutions per minute (rpm) for 10 min at 4 degrees C. Transfer the aqueous phase to a new 1.5 mL tube and add 1 volume of cold isopropanol. Incubate the samples for 20-30 min at RT and centrifuge at 10,000 rpm for 10 min at 4 degrees C. Wash pellet with Sigma-grade 80 percent ethanol. Remove the supernatant and briefly air-dry the pellet. Dissolve the RNA pellet in approximately 200 microliters of DEPC treated water. Heat briefly at 65 degrees C. to dissolve pellet and vortex or pipet to resuspend RNA pellet. Adjust RNA concentration to 1-2 microgram/microliter.

DNA is extracted using EZNA SP Plant DNA Mini kit (Omega Biotek, Norcross Ga., Cat # D5511) and Lysing Matrix E tubes (Q-Biogen, Cat #6914), following the manufacturer's protocol or modifications thereof by those skilled in the art of polynucleotide extraction that may enhance recovery or purity of the extracted DNA. Briefly, aliquot ground tissue to a Lysing Matrix E tube on dry ice, add 800 μl Buffer SP1 to each sample, homogenize in a bead beater for 35-45 sec, incubate on ice for 45-60 sec, centrifuge at ≥14000 rpm for 1 min at RT, add 10 microliter RNase A to the lysate, incubate at 65° C. for 10 min, centrifuge for 1 min at RT, add 280 μl Buffer SP2 and vortex to mix, incubate the samples on ice for 5 min, centrifuge at ≥10,000 g for 10 min at RT, transfer the supernatant to a homogenizer column in a 2 ml collection tube, centrifuge at 10,000 g for 2 min at RT, transfer the cleared lysate into a 1.5 ml microfuge tube, add 1.5 volumes Buffer SP3 to the cleared lysate, vortex immediately to obtain a homogeneous mixture, transfer up to 650 μl supernatant to the Hi-Bind column, centrifuge at 10,000 g for 1 min, repeat, apply 100 μl 65° C. Elution Buffer to the column, centrifuge at 10,000 g for 5 min at RT.

Next-generation DNA sequencers, such as the 454-FLX (Roche, Branford, Conn.), the SOLiD (Applied Biosystems), and the Genome Analyzer (HiSeq2000, Illumina, San Diego, Calif.) are used to provide polynucleotide sequences from the DNA and RNA extracted from the plant tissues. Raw sequence data is assembled into contigs. The contig sequence is used to identify trigger molecules that can be applied to the plant to enable regulation of the gene expression.

The target DNA sequence isolated from genomic (gDNA) and coding DNA (cDNA) from the various weedy plant species for the PPG oxidase gene and the assembled contigs as set forth in SEQ ID Nos: 1-71 and Table 1 below.

TABLE 1 SEQ ID NO SPECIES TYPE LENGTH 1 Amaranthus albus cDNA Contig 1200 2 Amaranthus albus cDNA Contig 487 3 Amaranthus graecizans cDNA Contig 1847 4 Amaranthus hybridus cDNA Contig 1170 5 Amaranthus lividus cDNA Contig 2344 6 Amaranthus palmeri cDNA Contig 1866 7 Amaranthus palmeri cDNA Contig 1066 8 Amaranthus palmeri cDNA Contig 294 9 Amaranthus palmeri gDNA Contig 6448 10 Amaranthus palmeri gDNA Contig 5240 11 Amaranthus palmeri gDNA Contig 2936 12 Amaranthus palmeri gDNA Contig 1923 13 Amaranthus palmeri gDNA Contig 2787 14 Amaranthus palmeri gDNA Contig 2379 15 Amaranthus palmeri gDNA Contig 760 16 Amaranthus rudis cDNA Contig 1872 17 Amaranthus rudis cDNA Contig 1605 18 Amaranthus rudis cDNA Contig 1238 19 Amaranthus rudis cDNA Contig 635 20 Amaranthus rudis gDNA Contig 5704 21 Amaranthus rudis gDNA Contig 1031 22 Amaranthus rudis gDNA Contig 1017 23 Amaranthus rudis gDNA Contig 630 24 Amaranthus rudis gDNA Contig 612 25 Amaranthus rudis gDNA Contig 487 26 Amaranthus spinosus cDNA Contig 1582 27 Amaranthus thunbergii cDNA Contig 1485 28 Amaranthus viridis cDNA Contig 1808 29 Ambrosia trifida cDNA Contig 1829 30 Ambrosia trifida cDNA Contig 921 31 Ambrosia trifida cDNA Contig 811 32 Ambrosia trifida gDNA Contig 4915 33 Ambrosia trifida gDNA Contig 851 34 Ambrosia trifida gDNA Contig 507 35 Chenopodium album cDNA Contig 1648 36 Conyza canadensis cDNA Contig 1653 37 Conyza canadensis cDNA Contig 600 38 Conyza canadensis gDNA Contig 10631 39 Conyza canadensis gDNA Contig 10185 40 Conyza canadensis gDNA Contig 2634 41 Euphorbia heterophylla cDNA Contig 1631 42 Euphorbia heterophylla cDNA Contig 480 43 Euphorbia heterophylla gDNA Contig 4067 44 Euphorbia heterophylla gDNA Contig 2630 45 Euphorbia heterophylla gDNA Contig 1010 46 Euphorbia heterophylla gDNA Contig 855 47 Euphorbia heterophylla gDNA Contig 121 48 Euphorbia heterophylla gDNA Contig 77 49 Commelina diffusa cDNA Contig 444 50 Commelina diffusa gDNA Contig 3043 51 Commelina diffusa gDNA Contig 1559 52 Commelina diffusa gDNA Contig 589 53 Digitaria sanguinalis cDNA Contig 876 54 Digitaria sanguinalis cDNA Contig 518 55 Digitaria sanguinalis cDNA Contig 387 56 Digitaria sanguinalis gDNA Contig 2697 57 Digitaria sanguinalis gDNA Contig 1089 58 Kochia scoparia cDNA Contig 1518 59 Kochia scoparia cDNA Contig 963 60 Kochia scoparia cDNA Contig 564 61 Kochia scoparia cDNA Contig 516 62 Lolium multiflorum cDNA Contig 1185 63 Lolium multiflorum cDNA Contig 258 64 Lolium multiflorum cDNA Contig 201 65 Lolium multiflorum cDNA Contig 150 66 Lolium multiflorum cDNA Contig 144 67 Lolium multiflorum cDNA Contig 114 68 Lolium multiflorum cDNA Contig 108 69 Lolium multiflorum gDNA Contig 3037 70 Lolium multiflorum gDNA Contig 795 71 Lolium multiflorum gDNA Contig 381

Example 2. Polynucleotides Related to the Trigger Molecules

The gene sequences and fragments of Table 1 were divided into 200 polynucleotide (200-mer) lengths with 25 polynucleotide overlapping regions and are shown in Table 2, SEQ ID NOs:72-429. These polynucleotides are tested to select the most efficacious trigger regions across the length of any target sequence. The trigger polynucleotides are constructed as sense or anti-sense ssDNA or ssRNA, dsRNA or dsDNA, or dsDNA/RNA hybrids and combined with an organosilicone based transfer agent to provide a polynucleotide preparation. The polynucleotides are combined into sets of two to three polynucleotides per set, using 4-8 nmol of each polynucleotide. Each polynucleotide set is prepared with the transfer agent and applied to a plant or a field of plants in combination with a PPG oxidase inhibitor containing herbicide, or followed by a PPG oxidase inhibitor treatment one to three days after the polynucleotide application, to determine the effect on the plant's susceptibility to a PPG oxidase inhibitor. The effect is measured as stunting the growth and/or killing of the plant and is measured 8-14 days after treatment with the polynucleotide set and PPG oxidase inhibitor. The most efficacious sets are identified and the individual polynucleotides are tested in the same methods as the sets are and the most efficacious single 200-mer identified. The 200-mer sequence is divided into smaller sequences of 50-70-mer regions with 10-15 polynucleotide overlapping regions and the polynucleotides tested individually. The most efficacious 50-70-mer is further divided into smaller sequences of 25-mer regions with a 12 to 13 polynucleotide overlapping region and tested for efficacy in combination with PPG oxidase inhibitor treatment. By this method, it is possible to identify an oligonucleotide or several oligonucleotides that are the most efficacious trigger molecule to effect plant sensitivity to a PPG oxidase inhibitor or modulation of a PPG oxidase gene expression. The modulation of PPG oxidase gene expression is determined by the detection of PPG oxidase siRNA molecules specific to a PPG oxidase gene or by an observation of a reduction in the amount of PPG oxidase RNA transcript produced relative to an untreated plant or by merely observing the anticipated phenotype of the application of the trigger with the PPG oxidase inhibitor containing herbicide. Detection of siRNA can be accomplished, for example, using kits such as mirVana (Ambion, Austin Tex.) and mirPremier (Sigma-Aldrich, St Louis, Mo.).

The target DNA sequence isolated from genomic (gDNA) and coding DNA (cDNA) from the various weedy plant species for the PPG oxidase gene and the assembled contigs as set forth in SEQ ID Nos: 1-71 were divided into polynucleotide fragments as shown in Table 2 below and as set forth in SEQ ID Nos: 72-1381.

TABLE 2 SEQ Name | ID NO Species Reference Type Start End 72 Amaranthus albus PPOX_B1_1 cDNA Contig 1 200 73 Amaranthus albus PPOX_B1_1 cDNA Contig 1 200 74 Amaranthus albus PPOX_B1_2 cDNA Contig 176 375 75 Amaranthus albus PPOX_B1_2 cDNA Contig 176 375 76 Amaranthus albus PPOX_B1_3 cDNA Contig 351 550 77 Amaranthus albus PPOX_B1_3 cDNA Contig 351 550 78 Amaranthus albus PPOX_B1_4 cDNA Contig 526 725 79 Amaranthus albus PPOX_B1_4 cDNA Contig 526 725 80 Amaranthus albus PPOX_B1_5 cDNA Contig 701 900 81 Amaranthus albus PPOX_B1_5 cDNA Contig 701 900 82 Amaranthus albus PPOX_B1_6 cDNA Contig 876 1075 83 Amaranthus albus PPOX_B1_6 cDNA Contig 876 1075 84 Amaranthus albus PPOX_B2_1 cDNA Contig 1 200 85 Amaranthus albus PPOX_B2_1 cDNA Contig 1 200 86 Amaranthus albus PPOX_B2_2 cDNA Contig 176 375 87 Amaranthus albus PPOX_B2_2 cDNA Contig 176 375 88 Amaranthus graecizans PPOX_B3_1 cDNA Contig 1 200 89 Amaranthus graecizans PPOX_B3_1 cDNA Contig 1 200 90 Amaranthus graecizans PPOX_B3_2 cDNA Contig 176 375 91 Amaranthus graecizans PPOX_B3_2 cDNA Contig 176 375 92 Amaranthus graecizans PPOX_B3_3 cDNA Contig 351 550 93 Amaranthus graecizans PPOX_B3_3 cDNA Contig 351 550 94 Amaranthus graecizans PPOX_B3_4 cDNA Contig 526 725 95 Amaranthus graecizans PPOX_B3_4 cDNA Contig 526 725 96 Amaranthus graecizans PPOX_B3_5 cDNA Contig 701 900 97 Amaranthus graecizans PPOX_B3_5 cDNA Contig 701 900 98 Amaranthus graecizans PPOX_B3_6 cDNA Contig 876 1075 99 Amaranthus graecizans PPOX_B3_6 cDNA Contig 876 1075 100 Amaranthus graecizans PPOX_B3_7 cDNA Contig 1051 1250 101 Amaranthus graecizans PPOX_B3_7 cDNA Contig 1051 1250 102 Amaranthus graecizans PPOX_B3_8 cDNA Contig 1226 1425 103 Amaranthus graecizans PPOX_B3_8 cDNA Contig 1226 1425 104 Amaranthus graecizans PPOX_B3_9 cDNA Contig 1401 1600 105 Amaranthus graecizans PPOX_B3_9 cDNA Contig 1401 1600 106 Amaranthus graecizans PPOX_B3_10 cDNA Contig 1576 1775 107 Amaranthus graecizans PPOX_B3_10 cDNA Contig 1576 1775 108 Amaranthus hybridus PPOX_B4_1 cDNA Contig 1 200 109 Amaranthus hybridus PPOX_B4_1 cDNA Contig 1 200 110 Amaranthus hybridus PPOX_B4_2 cDNA Contig 176 375 111 Amaranthus hybridus PPOX_B4_2 cDNA Contig 176 375 112 Amaranthus hybridus PPOX_B4_3 cDNA Contig 351 550 113 Amaranthus hybridus PPOX_B4_3 cDNA Contig 351 550 114 Amaranthus hybridus PPOX_B4_4 cDNA Contig 526 725 115 Amaranthus hybridus PPOX_B4_4 cDNA Contig 526 725 116 Amaranthus hybridus PPOX_B4_5 cDNA Contig 701 900 117 Amaranthus hybridus PPOX_B4_5 cDNA Contig 701 900 118 Amaranthus hybridus PPOX_B4_6 cDNA Contig 876 1075 119 Amaranthus hybridus PPOX_B4_6 cDNA Contig 876 1075 120 Amaranthus lividus PPOX_B5_1 cDNA Contig 1 200 121 Amaranthus lividus PPOX_B5_1 cDNA Contig 1 200 122 Amaranthus lividus PPOX_B5_2 cDNA Contig 176 375 123 Amaranthus lividus PPOX_B5_2 cDNA Contig 176 375 124 Amaranthus lividus PPOX_B5_3 cDNA Contig 351 550 125 Amaranthus lividus PPOX_B5_3 cDNA Contig 351 550 126 Amaranthus lividus PPOX_B5_4 cDNA Contig 526 725 127 Amaranthus lividus PPOX_B5_4 cDNA Contig 526 725 128 Amaranthus lividus PPOX_B5_5 cDNA Contig 701 900 129 Amaranthus lividus PPOX_B5_5 cDNA Contig 701 900 130 Amaranthus lividus PPOX_B5_6 cDNA Contig 876 1075 131 Amaranthus lividus PPOX_B5_6 cDNA Contig 876 1075 132 Amaranthus lividus PPOX_B5_7 cDNA Contig 1051 1250 133 Amaranthus lividus PPOX_B5_7 cDNA Contig 1051 1250 134 Amaranthus lividus PPOX_B5_8 cDNA Contig 1226 1425 135 Amaranthus lividus PPOX_B5_8 cDNA Contig 1226 1425 136 Amaranthus lividus PPOX_B5_9 cDNA Contig 1401 1600 137 Amaranthus lividus PPOX_B5_9 cDNA Contig 1401 1600 138 Amaranthus lividus PPOX_B5_10 cDNA Contig 1576 1775 139 Amaranthus lividus PPOX_B5_10 cDNA Contig 1576 1775 140 Amaranthus lividus PPOX_B5_11 cDNA Contig 1751 1950 141 Amaranthus lividus PPOX_B5_11 cDNA Contig 1751 1950 142 Amaranthus lividus PPOX_B5_12 cDNA Contig 1926 2125 143 Amaranthus lividus PPOX_B5_12 cDNA Contig 1926 2125 144 Amaranthus lividus PPOX_B5_13 cDNA Contig 2101 2300 145 Amaranthus lividus PPOX_B5_13 cDNA Contig 2101 2300 146 Amaranthus palmeri PPOX_B6_1 cDNA Contig 1 200 147 Amaranthus palmeri PPOX_B6_1 cDNA Contig 1 200 148 Amaranthus palmeri PPOX_B6_2 cDNA Contig 176 375 149 Amaranthus palmeri PPOX_B6_2 cDNA Contig 176 375 150 Amaranthus palmeri PPOX_B6_3 cDNA Contig 351 550 151 Amaranthus palmeri PPOX_B6_3 cDNA Contig 351 550 152 Amaranthus palmeri PPOX_B6_4 cDNA Contig 526 725 153 Amaranthus palmeri PPOX_B6_4 cDNA Contig 526 725 154 Amaranthus palmeri PPOX_B6_5 cDNA Contig 701 900 155 Amaranthus palmeri PPOX_B6_5 cDNA Contig 701 900 156 Amaranthus palmeri PPOX_B6_6 cDNA Contig 876 1075 157 Amaranthus palmeri PPOX_B6_6 cDNA Contig 876 1075 158 Amaranthus palmeri PPOX_B6_7 cDNA Contig 1051 1250 159 Amaranthus palmeri PPOX_B6_7 cDNA Contig 1051 1250 160 Amaranthus palmeri PPOX_B6_8 cDNA Contig 1226 1425 161 Amaranthus palmeri PPOX_B6_8 cDNA Contig 1226 1425 162 Amaranthus palmeri PPOX_B6_9 cDNA Contig 1401 1600 163 Amaranthus palmeri PPOX_B6_9 cDNA Contig 1401 1600 164 Amaranthus palmeri PPOX_B6_10 cDNA Contig 1576 1775 165 Amaranthus palmeri PPOX_B6_10 cDNA Contig 1576 1775 166 Amaranthus palmeri PPOX_B13_1 gDNA Contig 1 200 167 Amaranthus palmeri PPOX_B13_1 gDNA Contig 1 200 168 Amaranthus palmeri PPOX_B13_2 gDNA Contig 176 375 169 Amaranthus palmeri PPOX_B13_2 gDNA Contig 176 375 170 Amaranthus palmeri PPOX_B13_3 gDNA Contig 351 550 171 Amaranthus palmeri PPOX_B13_3 gDNA Contig 351 550 172 Amaranthus palmeri PPOX_B13_4 gDNA Contig 526 725 173 Amaranthus palmeri PPOX_B13_4 gDNA Contig 526 725 174 Amaranthus palmeri PPOX_B13_5 gDNA Contig 701 900 175 Amaranthus palmeri PPOX_B13_5 gDNA Contig 701 900 176 Amaranthus palmeri PPOX_B13_6 gDNA Contig 876 1075 177 Amaranthus palmeri PPOX_B13_6 gDNA Contig 876 1075 178 Amaranthus palmeri PPOX_B13_7 gDNA Contig 1051 1250 179 Amaranthus palmeri PPOX_B13_7 gDNA Contig 1051 1250 180 Amaranthus palmeri PPOX_B13_8 gDNA Contig 1226 1425 181 Amaranthus palmeri PPOX_B13_8 gDNA Contig 1226 1425 182 Amaranthus palmeri PPOX_B13_9 gDNA Contig 1401 1600 183 Amaranthus palmeri PPOX_B13_9 gDNA Contig 1401 1600 184 Amaranthus palmeri PPOX_B13_10 gDNA Contig 1576 1775 185 Amaranthus palmeri PPOX_B13_10 gDNA Contig 1576 1775 186 Amaranthus palmeri PPOX_B13_11 gDNA Contig 1751 1950 187 Amaranthus palmeri PPOX_B13_11 gDNA Contig 1751 1950 188 Amaranthus palmeri PPOX_B13_12 gDNA Contig 1926 2125 189 Amaranthus palmeri PPOX_B13_12 gDNA Contig 1926 2125 190 Amaranthus palmeri PPOX_B13_13 gDNA Contig 2101 2300 191 Amaranthus palmeri PPOX_B13_13 gDNA Contig 2101 2300 192 Amaranthus palmeri PPOX_B13_14 gDNA Contig 2276 2475 193 Amaranthus palmeri PPOX_B13_14 gDNA Contig 2276 2475 194 Amaranthus palmeri PPOX_B13_15 gDNA Contig 2451 2650 195 Amaranthus palmeri PPOX_B13_15 gDNA Contig 2451 2650 196 Amaranthus palmeri PPOX_B10_1 gDNA Contig 1 200 197 Amaranthus palmeri PPOX_B10_1 gDNA Contig 1 200 198 Amaranthus palmeri PPOX_B10_2 gDNA Contig 176 375 199 Amaranthus palmeri PPOX_B10_2 gDNA Contig 176 375 200 Amaranthus palmeri PPOX_B10_3 gDNA Contig 351 550 201 Amaranthus palmeri PPOX_B10_3 gDNA Contig 351 550 202 Amaranthus palmeri PPOX_B10_4 gDNA Contig 526 725 203 Amaranthus palmeri PPOX_B10_4 gDNA Contig 526 725 204 Amaranthus palmeri PPOX_B10_5 gDNA Contig 701 900 205 Amaranthus palmeri PPOX_B10_5 gDNA Contig 701 900 206 Amaranthus palmeri PPOX_B10_6 gDNA Contig 876 1075 207 Amaranthus palmeri PPOX_B10_6 gDNA Contig 876 1075 208 Amaranthus palmeri PPOX_B10_7 gDNA Contig 1051 1250 209 Amaranthus palmeri PPOX_B10_7 gDNA Contig 1051 1250 210 Amaranthus palmeri PPOX_B10_8 gDNA Contig 1226 1425 211 Amaranthus palmeri PPOX_B10_8 gDNA Contig 1226 1425 212 Amaranthus palmeri PPOX_B10_9 gDNA Contig 1401 1600 213 Amaranthus palmeri PPOX_B10_9 gDNA Contig 1401 1600 214 Amaranthus palmeri PPOX_B10_10 gDNA Contig 1576 1775 215 Amaranthus palmeri PPOX_B10_10 gDNA Contig 1576 1775 216 Amaranthus palmeri PPOX_B10_11 gDNA Contig 1751 1950 217 Amaranthus palmeri PPOX_B10_11 gDNA Contig 1751 1950 218 Amaranthus palmeri PPOX_B10_12 gDNA Contig 1926 2125 219 Amaranthus palmeri PPOX_B10_12 gDNA Contig 1926 2125 220 Amaranthus palmeri PPOX_B10_13 gDNA Contig 2101 2300 221 Amaranthus palmeri PPOX_B10_13 gDNA Contig 2101 2300 222 Amaranthus palmeri PPOX_B10_14 gDNA Contig 2276 2475 223 Amaranthus palmeri PPOX_B10_14 gDNA Contig 2276 2475 224 Amaranthus palmeri PPOX_B10_15 gDNA Contig 2451 2650 225 Amaranthus palmeri PPOX_B10_15 gDNA Contig 2451 2650 226 Amaranthus palmeri PPOX_B10_16 gDNA Contig 2626 2825 227 Amaranthus palmeri PPOX_B10_16 gDNA Contig 2626 2825 228 Amaranthus palmeri PPOX_B10_17 gDNA Contig 2801 3000 229 Amaranthus palmeri PPOX_B10_17 gDNA Contig 2801 3000 230 Amaranthus palmeri PPOX_B10_18 gDNA Contig 2976 3175 231 Amaranthus palmeri PPOX_B10_18 gDNA Contig 2976 3175 232 Amaranthus palmeri PPOX_B10_19 gDNA Contig 3151 3350 233 Amaranthus palmeri PPOX_B10_19 gDNA Contig 3151 3350 234 Amaranthus palmeri PPOX_B10_20 gDNA Contig 3326 3525 235 Amaranthus palmeri PPOX_B10_20 gDNA Contig 3326 3525 236 Amaranthus palmeri PPOX_B10_21 gDNA Contig 3501 3700 237 Amaranthus palmeri PPOX_B10_21 gDNA Contig 3501 3700 238 Amaranthus palmeri PPOX_B10_22 gDNA Contig 3676 3875 239 Amaranthus palmeri PPOX_B10_22 gDNA Contig 3676 3875 240 Amaranthus palmeri PPOX_B10_23 gDNA Contig 3851 4050 241 Amaranthus palmeri PPOX_B10_23 gDNA Contig 3851 4050 242 Amaranthus palmeri PPOX_B10_24 gDNA Contig 4026 4225 243 Amaranthus palmeri PPOX_B10_24 gDNA Contig 4026 4225 244 Amaranthus palmeri PPOX_B10_25 gDNA Contig 4201 4400 245 Amaranthus palmeri PPOX_B10_25 gDNA Contig 4201 4400 246 Amaranthus palmeri PPOX_B10_26 gDNA Contig 4376 4575 247 Amaranthus palmeri PPOX_B10_26 gDNA Contig 4376 4575 248 Amaranthus palmeri PPOX_B10_27 gDNA Contig 4551 4750 249 Amaranthus palmeri PPOX_B10_27 gDNA Contig 4551 4750 250 Amaranthus palmeri PPOX_B10_28 gDNA Contig 4726 4925 251 Amaranthus palmeri PPOX_B10_28 gDNA Contig 4726 4925 252 Amaranthus palmeri PPOX_B10_29 gDNA Contig 4901 5100 253 Amaranthus palmeri PPOX_B10_29 gDNA Contig 4901 5100 254 Amaranthus palmeri PPOX_B14_1 gDNA Contig 1 200 255 Amaranthus palmeri PPOX_B14_1 gDNA Contig 1 200 256 Amaranthus palmeri PPOX_B14_2 gDNA Contig 176 375 257 Amaranthus palmeri PPOX_B14_2 gDNA Contig 176 375 258 Amaranthus palmeri PPOX_B14_3 gDNA Contig 351 550 259 Amaranthus palmeri PPOX_B14_3 gDNA Contig 351 550 260 Amaranthus palmeri PPOX_B14_4 gDNA Contig 526 725 261 Amaranthus palmeri PPOX_B14_4 gDNA Contig 526 725 262 Amaranthus palmeri PPOX_B14_5 gDNA Contig 701 900 263 Amaranthus palmeri PPOX_B14_5 gDNA Contig 701 900 264 Amaranthus palmeri PPOX_B14_6 gDNA Contig 876 1075 265 Amaranthus palmeri PPOX_B14_6 gDNA Contig 876 1075 266 Amaranthus palmeri PPOX_B14_7 gDNA Contig 1051 1250 267 Amaranthus palmeri PPOX_B14_7 gDNA Contig 1051 1250 268 Amaranthus palmeri PPOX_B14_8 gDNA Contig 1226 1425 269 Amaranthus palmeri PPOX_B14_8 gDNA Contig 1226 1425 270 Amaranthus palmeri PPOX_B14_9 gDNA Contig 1401 1600 271 Amaranthus palmeri PPOX_B14_9 gDNA Contig 1401 1600 272 Amaranthus palmeri PPOX_B14_10 gDNA Contig 1576 1775 273 Amaranthus palmeri PPOX_B14_10 gDNA Contig 1576 1775 274 Amaranthus palmeri PPOX_B14_11 gDNA Contig 1751 1950 275 Amaranthus palmeri PPOX_B14_11 gDNA Contig 1751 1950 276 Amaranthus palmeri PPOX_B14_12 gDNA Contig 1926 2125 277 Amaranthus palmeri PPOX_B14_12 gDNA Contig 1926 2125 278 Amaranthus palmeri PPOX_B14_13 gDNA Contig 2101 2300 279 Amaranthus palmeri PPOX_B14_13 gDNA Contig 2101 2300 280 Amaranthus palmeri PPOX_B7_1 cDNA Contig 1 200 281 Amaranthus palmeri PPOX_B7_1 cDNA Contig 1 200 282 Amaranthus palmeri PPOX_B7_2 cDNA Contig 176 375 283 Amaranthus palmeri PPOX_B7_2 cDNA Contig 176 375 284 Amaranthus palmeri PPOX_B7_3 cDNA Contig 351 550 285 Amaranthus palmeri PPOX_B7_3 cDNA Contig 351 550 286 Amaranthus palmeri PPOX_B7_4 cDNA Contig 526 725 287 Amaranthus palmeri PPOX_B7_4 cDNA Contig 526 725 288 Amaranthus palmeri PPOX_B7_5 cDNA Contig 701 900 289 Amaranthus palmeri PPOX_B7_5 cDNA Contig 701 900 290 Amaranthus palmeri PPOX_B8_1 cDNA Contig 1 200 291 Amaranthus palmeri PPOX_B8_1 cDNA Contig 1 200 292 Amaranthus palmeri PPOX_B9_1 gDNA Contig 1 200 293 Amaranthus palmeri PPOX_B9_1 gDNA Contig 1 200 294 Amaranthus palmeri PPOX_B9_2 gDNA Contig 176 375 295 Amaranthus palmeri PPOX_B9_2 gDNA Contig 176 375 296 Amaranthus palmeri PPOX_B9_3 gDNA Contig 351 550 297 Amaranthus palmeri PPOX_B9_3 gDNA Contig 351 550 298 Amaranthus palmeri PPOX_B9_4 gDNA Contig 526 725 299 Amaranthus palmeri PPOX_B9_4 gDNA Contig 526 725 300 Amaranthus palmeri PPOX_B9_5 gDNA Contig 701 900 301 Amaranthus palmeri PPOX_B9_5 gDNA Contig 701 900 302 Amaranthus palmeri PPOX_B9_6 gDNA Contig 876 1075 303 Amaranthus palmeri PPOX_B9_6 gDNA Contig 876 1075 304 Amaranthus palmeri PPOX_B9_7 gDNA Contig 1051 1250 305 Amaranthus palmeri PPOX_B9_7 gDNA Contig 1051 1250 306 Amaranthus palmeri PPOX_B9_8 gDNA Contig 1226 1425 307 Amaranthus palmeri PPOX_B9_8 gDNA Contig 1226 1425 308 Amaranthus palmeri PPOX_B9_9 gDNA Contig 1401 1600 309 Amaranthus palmeri PPOX_B9_9 gDNA Contig 1401 1600 310 Amaranthus palmeri PPOX_B9_10 gDNA Contig 1576 1775 311 Amaranthus palmeri PPOX_B9_10 gDNA Contig 1576 1775 312 Amaranthus palmeri PPOX_B9_11 gDNA Contig 1751 1950 313 Amaranthus palmeri PPOX_B9_11 gDNA Contig 1751 1950 314 Amaranthus palmeri PPOX_B9_12 gDNA Contig 1926 2125 315 Amaranthus palmeri PPOX_B9_12 gDNA Contig 1926 2125 316 Amaranthus palmeri PPOX_B9_13 gDNA Contig 2101 2300 317 Amaranthus palmeri PPOX_B9_13 gDNA Contig 2101 2300 318 Amaranthus palmeri PPOX_B9_14 gDNA Contig 2276 2475 319 Amaranthus palmeri PPOX_B9_14 gDNA Contig 2276 2475 320 Amaranthus palmeri PPOX_B9_15 gDNA Contig 2451 2650 321 Amaranthus palmeri PPOX_B9_15 gDNA Contig 2451 2650 322 Amaranthus palmeri PPOX_B9_16 gDNA Contig 2626 2825 323 Amaranthus palmeri PPOX_B9_16 gDNA Contig 2626 2825 324 Amaranthus palmeri PPOX_B9_17 gDNA Contig 2801 3000 325 Amaranthus palmeri PPOX_B9_17 gDNA Contig 2801 3000 326 Amaranthus palmeri PPOX_B9_18 gDNA Contig 2976 3175 327 Amaranthus palmeri PPOX_B9_18 gDNA Contig 2976 3175 328 Amaranthus palmeri PPOX_B9_19 gDNA Contig 3151 3350 329 Amaranthus palmeri PPOX_B9_19 gDNA Contig 3151 3350 330 Amaranthus palmeri PPOX_B9_20 gDNA Contig 3326 3525 331 Amaranthus palmeri PPOX_B9_20 gDNA Contig 3326 3525 332 Amaranthus palmeri PPOX_B9_21 gDNA Contig 3501 3700 333 Amaranthus palmeri PPOX_B9_21 gDNA Contig 3501 3700 334 Amaranthus palmeri PPOX_B9_22 gDNA Contig 3676 3875 335 Amaranthus palmeri PPOX_B9_22 gDNA Contig 3676 3875 336 Amaranthus palmeri PPOX_B9_23 gDNA Contig 3851 4050 337 Amaranthus palmeri PPOX_B9_23 gDNA Contig 3851 4050 338 Amaranthus palmeri PPOX_B9_24 gDNA Contig 4026 4225 339 Amaranthus palmeri PPOX_B9_24 gDNA Contig 4026 4225 340 Amaranthus palmeri PPOX_B9_25 gDNA Contig 4201 4400 341 Amaranthus palmeri PPOX_B9_25 gDNA Contig 4201 4400 342 Amaranthus palmeri PPOX_B9_26 gDNA Contig 4376 4575 343 Amaranthus palmeri PPOX_B9_26 gDNA Contig 4376 4575 344 Amaranthus palmeri PPOX_B9_27 gDNA Contig 4551 4750 345 Amaranthus palmeri PPOX_B9_27 gDNA Contig 4551 4750 346 Amaranthus palmeri PPOX_B9_28 gDNA Contig 4726 4925 347 Amaranthus palmeri PPOX_B9_28 gDNA Contig 4726 4925 348 Amaranthus palmeri PPOX_B9_29 gDNA Contig 4901 5100 349 Amaranthus palmeri PPOX_B9_29 gDNA Contig 4901 5100 350 Amaranthus palmeri PPOX_B9_30 gDNA Contig 5076 5275 351 Amaranthus palmeri PPOX_B9_30 gDNA Contig 5076 5275 352 Amaranthus palmeri PPOX_B9_31 gDNA Contig 5251 5450 353 Amaranthus palmeri PPOX_B9_31 gDNA Contig 5251 5450 354 Amaranthus palmeri PPOX_B9_32 gDNA Contig 5426 5625 355 Amaranthus palmeri PPOX_B9_32 gDNA Contig 5426 5625 356 Amaranthus palmeri PPOX_B9_33 gDNA Contig 5601 5800 357 Amaranthus palmeri PPOX_B9_33 gDNA Contig 5601 5800 358 Amaranthus palmeri PPOX_B9_34 gDNA Contig 5776 5975 359 Amaranthus palmeri PPOX_B9_34 gDNA Contig 5776 5975 360 Amaranthus palmeri PPOX_B9_35 gDNA Contig 5951 6150 361 Amaranthus palmeri PPOX_B9_35 gDNA Contig 5951 6150 362 Amaranthus palmeri PPOX_B9_36 gDNA Contig 6126 6325 363 Amaranthus palmeri PPOX_B9_36 gDNA Contig 6126 6325 364 Amaranthus palmeri PPOX_B12_1 gDNA Contig 1 200 365 Amaranthus palmeri PPOX_B12_1 gDNA Contig 1 200 366 Amaranthus palmeri PPOX_B12_2 gDNA Contig 176 375 367 Amaranthus palmeri PPOX_B12_2 gDNA Contig 176 375 368 Amaranthus palmeri PPOX_B12_3 gDNA Contig 351 550 369 Amaranthus palmeri PPOX_B12_3 gDNA Contig 351 550 370 Amaranthus palmeri PPOX_B12_4 gDNA Contig 526 725 371 Amaranthus palmeri PPOX_B12_4 gDNA Contig 526 725 372 Amaranthus palmeri PPOX_B12_5 gDNA Contig 701 900 373 Amaranthus palmeri PPOX_B12_5 gDNA Contig 701 900 374 Amaranthus palmeri PPOX_B12_6 gDNA Contig 876 1075 375 Amaranthus palmeri PPOX_B12_6 gDNA Contig 876 1075 376 Amaranthus palmeri PPOX_B12_7 gDNA Contig 1051 1250 377 Amaranthus palmeri PPOX_B12_7 gDNA Contig 1051 1250 378 Amaranthus palmeri PPOX_B12_8 gDNA Contig 1226 1425 379 Amaranthus palmeri PPOX_B12_8 gDNA Contig 1226 1425 380 Amaranthus palmeri PPOX_B12_9 gDNA Contig 1401 1600 381 Amaranthus palmeri PPOX_B12_9 gDNA Contig 1401 1600 382 Amaranthus palmeri PPOX_B12_10 gDNA Contig 1576 1775 383 Amaranthus palmeri PPOX_B12_10 gDNA Contig 1576 1775 384 Amaranthus palmeri PPOX_B15_1 gDNA Contig 1 200 385 Amaranthus palmeri PPOX_B15_1 gDNA Contig 1 200 386 Amaranthus palmeri PPOX_B15_2 gDNA Contig 176 375 387 Amaranthus palmeri PPOX_B15_2 gDNA Contig 176 375 388 Amaranthus palmeri PPOX_B15_3 gDNA Contig 351 550 389 Amaranthus palmeri PPOX_B15_3 gDNA Contig 351 550 390 Amaranthus palmeri PPOX_B15_4 gDNA Contig 526 725 391 Amaranthus palmeri PPOX_B15_4 gDNA Contig 526 725 392 Amaranthus palmeri PPOX_B11_1 gDNA Contig 1 200 393 Amaranthus palmeri PPOX_B11_1 gDNA Contig 1 200 394 Amaranthus palmeri PPOX_B11_2 gDNA Contig 176 375 395 Amaranthus palmeri PPOX_B11_2 gDNA Contig 176 375 396 Amaranthus palmeri PPOX_B11_3 gDNA Contig 351 550 397 Amaranthus palmeri PPOX_B11_3 gDNA Contig 351 550 398 Amaranthus palmeri PPOX_B11_4 gDNA Contig 526 725 399 Amaranthus palmeri PPOX_B11_4 gDNA Contig 526 725 400 Amaranthus palmeri PPOX_B11_5 gDNA Contig 701 900 401 Amaranthus palmeri PPOX_B11_5 gDNA Contig 701 900 402 Amaranthus palmeri PPOX_B11_6 gDNA Contig 876 1075 403 Amaranthus palmeri PPOX_B11_6 gDNA Contig 876 1075 404 Amaranthus palmeri PPOX_B11_7 gDNA Contig 1051 1250 405 Amaranthus palmeri PPOX_B11_7 gDNA Contig 1051 1250 406 Amaranthus palmeri PPOX_B11_8 gDNA Contig 1226 1425 407 Amaranthus palmeri PPOX_B11_8 gDNA Contig 1226 1425 408 Amaranthus palmeri PPOX_B11_9 gDNA Contig 1401 1600 409 Amaranthus palmeri PPOX_B11_9 gDNA Contig 1401 1600 410 Amaranthus palmeri PPOX_B11_10 gDNA Contig 1576 1775 411 Amaranthus palmeri PPOX_B11_10 gDNA Contig 1576 1775 412 Amaranthus palmeri PPOX_B11_11 gDNA Contig 1751 1950 413 Amaranthus palmeri PPOX_B11_11 gDNA Contig 1751 1950 414 Amaranthus palmeri PPOX_B11_12 gDNA Contig 1926 2125 415 Amaranthus palmeri PPOX_B11_12 gDNA Contig 1926 2125 416 Amaranthus palmeri PPOX_B11_13 gDNA Contig 2101 2300 417 Amaranthus palmeri PPOX_B11_13 gDNA Contig 2101 2300 418 Amaranthus palmeri PPOX_B11_14 gDNA Contig 2276 2475 419 Amaranthus palmeri PPOX_B11_14 gDNA Contig 2276 2475 420 Amaranthus palmeri PPOX_B11_15 gDNA Contig 2451 2650 421 Amaranthus palmeri PPOX_B11_15 gDNA Contig 2451 2650 422 Amaranthus palmeri PPOX_B11_16 gDNA Contig 2626 2825 423 Amaranthus palmeri PPOX_B11_16 gDNA Contig 2626 2825 424 Amaranthus rudis PPOX_B24_1 gDNA Contig 1 200 425 Amaranthus rudis PPOX_B24_1 gDNA Contig 1 200 426 Amaranthus rudis PPOX_B24_2 gDNA Contig 176 375 427 Amaranthus rudis PPOX_B24_2 gDNA Contig 176 375 428 Amaranthus rudis PPOX_B24_3 gDNA Contig 351 550 429 Amaranthus rudis PPOX_B24_3 gDNA Contig 351 550 430 Amaranthus rudis PPOX_B22_1 gDNA Contig 1 200 431 Amaranthus rudis PPOX_B22_1 gDNA Contig 1 200 432 Amaranthus rudis PPOX_B22_2 gDNA Contig 176 375 433 Amaranthus rudis PPOX_B22_2 gDNA Contig 176 375 434 Amaranthus rudis PPOX_B22_3 gDNA Contig 351 550 435 Amaranthus rudis PPOX_B22_3 gDNA Contig 351 550 436 Amaranthus rudis PPOX_B22_4 gDNA Contig 526 725 437 Amaranthus rudis PPOX_B22_4 gDNA Contig 526 725 438 Amaranthus rudis PPOX_B22_5 gDNA Contig 701 900 439 Amaranthus rudis PPOX_B22_5 gDNA Contig 701 900 440 Amaranthus rudis PPOX_B17_1 cDNA Contig 1 200 441 Amaranthus rudis PPOX_B17_1 cDNA Contig 1 200 442 Amaranthus rudis PPOX_B17_2 cDNA Contig 176 375 443 Amaranthus rudis PPOX_B17_2 cDNA Contig 176 375 444 Amaranthus rudis PPOX_B17_3 cDNA Contig 351 550 445 Amaranthus rudis PPOX_B17_3 cDNA Contig 351 550 446 Amaranthus rudis PPOX_B17_4 cDNA Contig 526 725 447 Amaranthus rudis PPOX_B17_4 cDNA Contig 526 725 448 Amaranthus rudis PPOX_B17_5 cDNA Contig 701 900 449 Amaranthus rudis PPOX_B17_5 cDNA Contig 701 900 450 Amaranthus rudis PPOX_B17_6 cDNA Contig 876 1075 451 Amaranthus rudis PPOX_B17_6 cDNA Contig 876 1075 452 Amaranthus rudis PPOX_B17_7 cDNA Contig 1051 1250 453 Amaranthus rudis PPOX_B17_7 cDNA Contig 1051 1250 454 Amaranthus rudis PPOX_B17_8 cDNA Contig 1226 1425 455 Amaranthus rudis PPOX_B17_8 cDNA Contig 1226 1425 456 Amaranthus rudis PPOX_B17_9 cDNA Contig 1401 1600 457 Amaranthus rudis PPOX_B17_9 cDNA Contig 1401 1600 458 Amaranthus rudis PPOX_B20_1 gDNA Contig 1 200 459 Amaranthus rudis PPOX_B20_1 gDNA Contig 1 200 460 Amaranthus rudis PPOX_B20_2 gDNA Contig 176 375 461 Amaranthus rudis PPOX_B20_2 gDNA Contig 176 375 462 Amaranthus rudis PPOX_B20_3 gDNA Contig 351 550 463 Amaranthus rudis PPOX_B20_3 gDNA Contig 351 550 464 Amaranthus rudis PPOX_B20_4 gDNA Contig 526 725 465 Amaranthus rudis PPOX_B20_4 gDNA Contig 526 725 466 Amaranthus rudis PPOX_B20_5 gDNA Contig 701 900 467 Amaranthus rudis PPOX_B20_5 gDNA Contig 701 900 468 Amaranthus rudis PPOX_B20_6 gDNA Contig 876 1075 469 Amaranthus rudis PPOX_B20_6 gDNA Contig 876 1075 470 Amaranthus rudis PPOX_B20_7 gDNA Contig 1051 1250 471 Amaranthus rudis PPOX_B20_7 gDNA Contig 1051 1250 472 Amaranthus rudis PPOX_B20_8 gDNA Contig 1226 1425 473 Amaranthus rudis PPOX_B20_8 gDNA Contig 1226 1425 474 Amaranthus rudis PPOX_B20_9 gDNA Contig 1401 1600 475 Amaranthus rudis PPOX_B20_9 gDNA Contig 1401 1600 476 Amaranthus rudis PPOX_B20_10 gDNA Contig 1576 1775 477 Amaranthus rudis PPOX_B20_10 gDNA Contig 1576 1775 478 Amaranthus rudis PPOX_B20_11 gDNA Contig 1751 1950 479 Amaranthus rudis PPOX_B20_11 gDNA Contig 1751 1950 480 Amaranthus rudis PPOX_B20_12 gDNA Contig 1926 2125 481 Amaranthus rudis PPOX_B20_12 gDNA Contig 1926 2125 482 Amaranthus rudis PPOX_B20_13 gDNA Contig 2101 2300 483 Amaranthus rudis PPOX_B20_13 gDNA Contig 2101 2300 484 Amaranthus rudis PPOX_B20_14 gDNA Contig 2276 2475 485 Amaranthus rudis PPOX_B20_14 gDNA Contig 2276 2475 486 Amaranthus rudis PPOX_B20_15 gDNA Contig 2451 2650 487 Amaranthus rudis PPOX_B20_15 gDNA Contig 2451 2650 488 Amaranthus rudis PPOX_B20_16 gDNA Contig 2626 2825 489 Amaranthus rudis PPOX_B20_16 gDNA Contig 2626 2825 490 Amaranthus rudis PPOX_B20_17 gDNA Contig 2801 3000 491 Amaranthus rudis PPOX_B20_17 gDNA Contig 2801 3000 492 Amaranthus rudis PPOX_B20_18 gDNA Contig 2976 3175 493 Amaranthus rudis PPOX_B20_18 gDNA Contig 2976 3175 494 Amaranthus rudis PPOX_B20_19 gDNA Contig 3151 3350 495 Amaranthus rudis PPOX_B20_19 gDNA Contig 3151 3350 496 Amaranthus rudis PPOX_B20_20 gDNA Contig 3326 3525 497 Amaranthus rudis PPOX_B20_20 gDNA Contig 3326 3525 498 Amaranthus rudis PPOX_B20_21 gDNA Contig 3501 3700 499 Amaranthus rudis PPOX_B20_21 gDNA Contig 3501 3700 500 Amaranthus rudis PPOX_B20_22 gDNA Contig 3676 3875 501 Amaranthus rudis PPOX_B20_22 gDNA Contig 3676 3875 502 Amaranthus rudis PPOX_B20_23 gDNA Contig 3851 4050 503 Amaranthus rudis PPOX_B20_23 gDNA Contig 3851 4050 504 Amaranthus rudis PPOX_B20_24 gDNA Contig 4026 4225 505 Amaranthus rudis PPOX_B20_24 gDNA Contig 4026 4225 506 Amaranthus rudis PPOX_B20_25 gDNA Contig 4201 4400 507 Amaranthus rudis PPOX_B20_25 gDNA Contig 4201 4400 508 Amaranthus rudis PPOX_B20_26 gDNA Contig 4376 4575 509 Amaranthus rudis PPOX_B20_26 gDNA Contig 4376 4575 510 Amaranthus rudis PPOX_B20_27 gDNA Contig 4551 4750 511 Amaranthus rudis PPOX_B20_27 gDNA Contig 4551 4750 512 Amaranthus rudis PPOX_B20_28 gDNA Contig 4726 4925 513 Amaranthus rudis PPOX_B20_28 gDNA Contig 4726 4925 514 Amaranthus rudis PPOX_B20_29 gDNA Contig 4901 5100 515 Amaranthus rudis PPOX_B20_29 gDNA Contig 4901 5100 516 Amaranthus rudis PPOX_B20_30 gDNA Contig 5076 5275 517 Amaranthus rudis PPOX_B20_30 gDNA Contig 5076 5275 518 Amaranthus rudis PPOX_B20_31 gDNA Contig 5251 5450 519 Amaranthus rudis PPOX_B20_31 gDNA Contig 5251 5450 520 Amaranthus rudis PPOX_B20_32 gDNA Contig 5426 5625 521 Amaranthus rudis PPOX_B20_32 gDNA Contig 5426 5625 522 Amaranthus rudis PPOX_B18_1 cDNA Contig 1 200 523 Amaranthus rudis PPOX_B18_1 cDNA Contig 1 200 524 Amaranthus rudis PPOX_B18_2 cDNA Contig 176 375 525 Amaranthus rudis PPOX_B18_2 cDNA Contig 176 375 526 Amaranthus rudis PPOX_B18_3 cDNA Contig 351 550 527 Amaranthus rudis PPOX_B18_3 cDNA Contig 351 550 528 Amaranthus rudis PPOX_B18_4 cDNA Contig 526 725 529 Amaranthus rudis PPOX_B18_4 cDNA Contig 526 725 530 Amaranthus rudis PPOX_B18_5 cDNA Contig 701 900 531 Amaranthus rudis PPOX_B18_5 cDNA Contig 701 900 532 Amaranthus rudis PPOX_B18_6 cDNA Contig 876 1075 533 Amaranthus rudis PPOX_B18_6 cDNA Contig 876 1075 534 Amaranthus rudis PPOX_B16_1 cDNA Contig 1 200 535 Amaranthus rudis PPOX_B16_1 cDNA Contig 1 200 536 Amaranthus rudis PPOX_B16_2 cDNA Contig 176 375 537 Amaranthus rudis PPOX_B16_2 cDNA Contig 176 375 538 Amaranthus rudis PPOX_B16_3 cDNA Contig 351 550 539 Amaranthus rudis PPOX_B16_3 cDNA Contig 351 550 540 Amaranthus rudis PPOX_B16_4 cDNA Contig 526 725 541 Amaranthus rudis PPOX_B16_4 cDNA Contig 526 725 542 Amaranthus rudis PPOX_B16_5 cDNA Contig 701 900 543 Amaranthus rudis PPOX_B16_5 cDNA Contig 701 900 544 Amaranthus rudis PPOX_B16_6 cDNA Contig 876 1075 545 Amaranthus rudis PPOX_B16_6 cDNA Contig 876 1075 546 Amaranthus rudis PPOX_B16_7 cDNA Contig 1051 1250 547 Amaranthus rudis PPOX_B16_7 cDNA Contig 1051 1250 548 Amaranthus rudis PPOX_B16_8 cDNA Contig 1226 1425 549 Amaranthus rudis PPOX_B16_8 cDNA Contig 1226 1425 550 Amaranthus rudis PPOX_B16_9 cDNA Contig 1401 1600 551 Amaranthus rudis PPOX_B16_9 cDNA Contig 1401 1600 552 Amaranthus rudis PPOX_B16_10 cDNA Contig 1576 1775 553 Amaranthus rudis PPOX_B16_10 cDNA Contig 1576 1775 554 Amaranthus rudis PPOX_B19_1 cDNA Contig 1 200 555 Amaranthus rudis PPOX_B19_1 cDNA Contig 1 200 556 Amaranthus rudis PPOX_B19_2 cDNA Contig 176 375 557 Amaranthus rudis PPOX_B19_2 cDNA Contig 176 375 558 Amaranthus rudis PPOX_B19_3 cDNA Contig 351 550 559 Amaranthus rudis PPOX_B19_3 cDNA Contig 351 550 560 Amaranthus rudis PPOX_B23_1 gDNA Contig 1 200 561 Amaranthus rudis PPOX_B23_1 gDNA Contig 1 200 562 Amaranthus rudis PPOX_B23_2 gDNA Contig 351 550 563 Amaranthus rudis PPOX_B23_2 gDNA Contig 351 550 564 Amaranthus rudis PPOX_B25_1 gDNA Contig 1 200 565 Amaranthus rudis PPOX_B25_1 gDNA Contig 1 200 566 Amaranthus rudis PPOX_B25_2 gDNA Contig 176 375 567 Amaranthus rudis PPOX_B25_2 gDNA Contig 176 375 568 Amaranthus rudis PPOX_B21_1 gDNA Contig 1 200 569 Amaranthus rudis PPOX_B21_1 gDNA Contig 1 200 570 Amaranthus rudis PPOX_B21_2 gDNA Contig 176 375 571 Amaranthus rudis PPOX_B21_2 gDNA Contig 176 375 572 Amaranthus rudis PPOX_B21_3 gDNA Contig 351 550 573 Amaranthus rudis PPOX_B21_3 gDNA Contig 351 550 574 Amaranthus rudis PPOX_B21_4 gDNA Contig 526 725 575 Amaranthus rudis PPOX_B21_4 gDNA Contig 526 725 576 Amaranthus rudis PPOX_B21_5 gDNA Contig 701 900 577 Amaranthus rudis PPOX_B21_5 gDNA Contig 701 900 578 Amaranthus spinosus PPOX_B26_1 cDNA Contig 1 200 579 Amaranthus spinosus PPOX_B26_1 cDNA Contig 1 200 580 Amaranthus spinosus PPOX_B26_2 cDNA Contig 176 375 581 Amaranthus spinosus PPOX_B26_2 cDNA Contig 176 375 582 Amaranthus spinosus PPOX_B26_3 cDNA Contig 351 550 583 Amaranthus spinosus PPOX_B26_3 cDNA Contig 351 550 584 Amaranthus spinosus PPOX_B26_4 cDNA Contig 526 725 585 Amaranthus spinosus PPOX_B26_4 cDNA Contig 526 725 586 Amaranthus spinosus PPOX_B26_5 cDNA Contig 701 900 587 Amaranthus spinosus PPOX_B26_5 cDNA Contig 701 900 588 Amaranthus spinosus PPOX_B26_6 cDNA Contig 876 1075 589 Amaranthus spinosus PPOX_B26_6 cDNA Contig 876 1075 590 Amaranthus spinosus PPOX_B26_7 cDNA Contig 1051 1250 591 Amaranthus spinosus PPOX_B26_7 cDNA Contig 1051 1250 592 Amaranthus spinosus PPOX_B26_8 cDNA Contig 1226 1425 593 Amaranthus spinosus PPOX_B26_8 cDNA Contig 1226 1425 594 Amaranthus thunbergii PPOX_B27_1 cDNA Contig 1 200 595 Amaranthus thunbergii PPOX_B27_1 cDNA Contig 1 200 596 Amaranthus thunbergii PPOX_B27_2 cDNA Contig 176 375 597 Amaranthus thunbergii PPOX_B27_2 cDNA Contig 176 375 598 Amaranthus thunbergii PPOX_B27_3 cDNA Contig 351 550 599 Amaranthus thunbergii PPOX_B27_3 cDNA Contig 351 550 600 Amaranthus thunbergii PPOX_B27_4 cDNA Contig 526 725 601 Amaranthus thunbergii PPOX_B27_4 cDNA Contig 526 725 602 Amaranthus thunbergii PPOX_B27_5 cDNA Contig 701 900 603 Amaranthus thunbergii PPOX_B27_5 cDNA Contig 701 900 604 Amaranthus thunbergii PPOX_B27_6 cDNA Contig 876 1075 605 Amaranthus thunbergii PPOX_B27_6 cDNA Contig 876 1075 606 Amaranthus thunbergii PPOX_B27_7 cDNA Contig 1051 1250 607 Amaranthus thunbergii PPOX_B27_7 cDNA Contig 1051 1250 608 Amaranthus thunbergii PPOX_B27_8 cDNA Contig 1226 1425 609 Amaranthus thunbergii PPOX_B27_8 cDNA Contig 1226 1425 610 Amaranthus viridis PPOX_B28_1 cDNA Contig 1 200 611 Amaranthus viridis PPOX_B28_1 cDNA Contig 1 200 612 Amaranthus viridis PPOX_B28_2 cDNA Contig 176 375 613 Amaranthus viridis PPOX_B28_2 cDNA Contig 176 375 614 Amaranthus viridis PPOX_B28_3 cDNA Contig 351 550 615 Amaranthus viridis PPOX_B28_3 cDNA Contig 351 550 616 Amaranthus viridis PPOX_B28_4 cDNA Contig 526 725 617 Amaranthus viridis PPOX_B28_4 cDNA Contig 526 725 618 Amaranthus viridis PPOX_B28_5 cDNA Contig 701 900 619 Amaranthus viridis PPOX_B28_5 cDNA Contig 701 900 620 Amaranthus viridis PPOX_B28_6 cDNA Contig 876 1075 621 Amaranthus viridis PPOX_B28_6 cDNA Contig 876 1075 622 Amaranthus viridis PPOX_B28_7 cDNA Contig 1051 1250 623 Amaranthus viridis PPOX_B28_7 cDNA Contig 1051 1250 624 Amaranthus viridis PPOX_B28_8 cDNA Contig 1226 1425 625 Amaranthus viridis PPOX_B28_8 cDNA Contig 1226 1425 626 Amaranthus viridis PPOX_B28_9 cDNA Contig 1401 1600 627 Amaranthus viridis PPOX_B28_9 cDNA Contig 1401 1600 628 Amaranthus viridis PPOX_B28_10 cDNA Contig 1576 1775 629 Amaranthus viridis PPOX_B28_10 cDNA Contig 1576 1775 630 Ambrosia trifida PPOX_B34_1 gDNA Contig 1 200 631 Ambrosia trifida PPOX_B34_1 gDNA Contig 1 200 632 Ambrosia trifida PPOX_B34_2 gDNA Contig 176 375 633 Ambrosia trifida PPOX_B34_2 gDNA Contig 176 375 634 Ambrosia trifida PPOX_B32_1 gDNA Contig 1 200 635 Ambrosia trifida PPOX_B32_1 gDNA Contig 1 200 636 Ambrosia trifida PPOX_B32_2 gDNA Contig 176 375 637 Ambrosia trifida PPOX_B32_2 gDNA Contig 176 375 638 Ambrosia trifida PPOX_B32_3 gDNA Contig 351 550 639 Ambrosia trifida PPOX_B32_3 gDNA Contig 351 550 640 Ambrosia trifida PPOX_B32_4 gDNA Contig 526 725 641 Ambrosia trifida PPOX_B32_4 gDNA Contig 526 725 642 Ambrosia trifida PPOX_B32_5 gDNA Contig 701 900 643 Ambrosia trifida PPOX_B32_5 gDNA Contig 701 900 644 Ambrosia trifida PPOX_B32_6 gDNA Contig 876 1075 645 Ambrosia trifida PPOX_B32_6 gDNA Contig 876 1075 646 Ambrosia trifida PPOX_B32_7 gDNA Contig 1051 1250 647 Ambrosia trifida PPOX_B32_7 gDNA Contig 1051 1250 648 Ambrosia trifida PPOX_B32_8 gDNA Contig 1226 1425 649 Ambrosia trifida PPOX_B32_8 gDNA Contig 1226 1425 650 Ambrosia trifida PPOX_B32_9 gDNA Contig 1401 1600 651 Ambrosia trifida PPOX_B32_9 gDNA Contig 1401 1600 652 Ambrosia trifida PPOX_B32_10 gDNA Contig 1576 1775 653 Ambrosia trifida PPOX_B32_10 gDNA Contig 1576 1775 654 Ambrosia trifida PPOX_B32_11 gDNA Contig 1751 1950 655 Ambrosia trifida PPOX_B32_11 gDNA Contig 1751 1950 656 Ambrosia trifida PPOX_B32_12 gDNA Contig 1926 2125 657 Ambrosia trifida PPOX_B32_12 gDNA Contig 1926 2125 658 Ambrosia trifida PPOX_B32_13 gDNA Contig 2101 2300 659 Ambrosia trifida PPOX_B32_13 gDNA Contig 2101 2300 660 Ambrosia trifida PPOX_B32_14 gDNA Contig 2276 2475 661 Ambrosia trifida PPOX_B32_14 gDNA Contig 2276 2475 662 Ambrosia trifida PPOX_B32_15 gDNA Contig 2451 2650 663 Ambrosia trifida PPOX_B32_15 gDNA Contig 2451 2650 664 Ambrosia trifida PPOX_B32_16 gDNA Contig 2626 2825 665 Ambrosia trifida PPOX_B32_16 gDNA Contig 2626 2825 666 Ambrosia trifida PPOX_B32_17 gDNA Contig 2801 3000 667 Ambrosia trifida PPOX_B32_17 gDNA Contig 2801 3000 668 Ambrosia trifida PPOX_B32_18 gDNA Contig 2976 3175 669 Ambrosia trifida PPOX_B32_18 gDNA Contig 2976 3175 670 Ambrosia trifida PPOX_B32_19 gDNA Contig 3151 3350 671 Ambrosia trifida PPOX_B32_19 gDNA Contig 3151 3350 672 Ambrosia trifida PPOX_B32_20 gDNA Contig 3326 3525 673 Ambrosia trifida PPOX_B32_20 gDNA Contig 3326 3525 674 Ambrosia trifida PPOX_B32_21 gDNA Contig 3501 3700 675 Ambrosia trifida PPOX_B32_21 gDNA Contig 3501 3700 676 Ambrosia trifida PPOX_B32_22 gDNA Contig 3676 3875 677 Ambrosia trifida PPOX_B32_22 gDNA Contig 3676 3875 678 Ambrosia trifida PPOX_B32_23 gDNA Contig 3851 4050 679 Ambrosia trifida PPOX_B32_23 gDNA Contig 3851 4050 680 Ambrosia trifida PPOX_B32_24 gDNA Contig 4026 4225 681 Ambrosia trifida PPOX_B32_24 gDNA Contig 4026 4225 682 Ambrosia trifida PPOX_B32_25 gDNA Contig 4201 4400 683 Ambrosia trifida PPOX_B32_25 gDNA Contig 4201 4400 684 Ambrosia trifida PPOX_B32_26 gDNA Contig 4376 4575 685 Ambrosia trifida PPOX_B32_26 gDNA Contig 4376 4575 686 Ambrosia trifida PPOX_B32_27 gDNA Contig 4551 4750 687 Ambrosia trifida PPOX_B32_27 gDNA Contig 4551 4750 688 Ambrosia trifida PPOX_B30_1 cDNA Contig 1 200 689 Ambrosia trifida PPOX_B30_1 cDNA Contig 1 200 690 Ambrosia trifida PPOX_B30_2 cDNA Contig 176 375 691 Ambrosia trifida PPOX_B30_2 cDNA Contig 176 375 692 Ambrosia trifida PPOX_B30_3 cDNA Contig 351 550 693 Ambrosia trifida PPOX_B30_3 cDNA Contig 351 550 694 Ambrosia trifida PPOX_B30_4 cDNA Contig 526 725 695 Ambrosia trifida PPOX_B30_4 cDNA Contig 526 725 696 Ambrosia trifida PPOX_B30_5 cDNA Contig 701 900 697 Ambrosia trifida PPOX_B30_5 cDNA Contig 701 900 698 Ambrosia trifida PPOX_B29_1 cDNA Contig 1 200 699 Ambrosia trifida PPOX_B29_1 cDNA Contig 1 200 700 Ambrosia trifida PPOX_B29_2 cDNA Contig 176 375 701 Ambrosia trifida PPOX_B29_2 cDNA Contig 176 375 702 Ambrosia trifida PPOX_B29_3 cDNA Contig 351 550 703 Ambrosia trifida PPOX_B29_3 cDNA Contig 351 550 704 Ambrosia trifida PPOX_B29_4 cDNA Contig 526 725 705 Ambrosia trifida PPOX_B29_4 cDNA Contig 526 725 706 Ambrosia trifida PPOX_B29_5 cDNA Contig 701 900 707 Ambrosia trifida PPOX_B29_5 cDNA Contig 701 900 708 Ambrosia trifida PPOX_B29_6 cDNA Contig 876 1075 709 Ambrosia trifida PPOX_B29_6 cDNA Contig 876 1075 710 Ambrosia trifida PPOX_B29_7 cDNA Contig 1051 1250 711 Ambrosia trifida PPOX_B29_7 cDNA Contig 1051 1250 712 Ambrosia trifida PPOX_B29_8 cDNA Contig 1226 1425 713 Ambrosia trifida PPOX_B29_8 cDNA Contig 1226 1425 714 Ambrosia trifida PPOX_B29_9 cDNA Contig 1401 1600 715 Ambrosia trifida PPOX_B29_9 cDNA Contig 1401 1600 716 Ambrosia trifida PPOX_B29_10 cDNA Contig 1576 1775 717 Ambrosia trifida PPOX_B29_10 cDNA Contig 1576 1775 718 Ambrosia trifida PPOX_B33_1 gDNA Contig 1 200 719 Ambrosia trifida PPOX_B33_1 gDNA Contig 1 200 720 Ambrosia trifida PPOX_B33_2 gDNA Contig 176 375 721 Ambrosia trifida PPOX_B33_2 gDNA Contig 176 375 722 Ambrosia trifida PPOX_B33_3 gDNA Contig 351 550 723 Ambrosia trifida PPOX_B33_3 gDNA Contig 351 550 724 Ambrosia trifida PPOX_B33_4 gDNA Contig 526 725 725 Ambrosia trifida PPOX_B33_4 gDNA Contig 526 725 726 Ambrosia trifida PPOX_B31_1 cDNA Contig 1 200 727 Ambrosia trifida PPOX_B31_1 cDNA Contig 1 200 728 Ambrosia trifida PPOX_B31_2 cDNA Contig 176 375 729 Ambrosia trifida PPOX_B31_2 cDNA Contig 176 375 730 Ambrosia trifida PPOX_B31_3 cDNA Contig 351 550 731 Ambrosia trifida PPOX_B31_3 cDNA Contig 351 550 732 Ambrosia trifida PPOX_B31_4 cDNA Contig 526 725 733 Ambrosia trifida PPOX_B31_4 cDNA Contig 526 725 734 Chenopodium album PPOX_B35_1 cDNA Contig 1 200 735 Chenopodium album PPOX_B35_1 cDNA Contig 1 200 736 Chenopodium album PPOX_B35_2 cDNA Contig 176 375 737 Chenopodium album PPOX_B35_2 cDNA Contig 176 375 738 Chenopodium album PPOX_B35_3 cDNA Contig 351 550 739 Chenopodium album PPOX_B35_3 cDNA Contig 351 550 740 Chenopodium album PPOX_B35_4 cDNA Contig 526 725 741 Chenopodium album PPOX_B35_4 cDNA Contig 526 725 742 Chenopodium album PPOX_B35_5 cDNA Contig 701 900 743 Chenopodium album PPOX_B35_5 cDNA Contig 701 900 744 Chenopodium album PPOX_B35_6 cDNA Contig 876 1075 745 Chenopodium album PPOX_B35_6 cDNA Contig 876 1075 746 Chenopodium album PPOX_B35_7 cDNA Contig 1051 1250 747 Chenopodium album PPOX_B35_7 cDNA Contig 1051 1250 748 Chenopodium album PPOX_B35_8 cDNA Contig 1226 1425 749 Chenopodium album PPOX_B35_8 cDNA Contig 1226 1425 750 Chenopodium album PPOX_B35_9 cDNA Contig 1401 1600 751 Chenopodium album PPOX_B35_9 cDNA Contig 1401 1600 752 Commelina diffusa PPOX_B50_1 gDNA Contig 1 200 753 Commelina diffusa PPOX_B50_1 gDNA Contig 1 200 754 Commelina diffusa PPOX_B50_2 gDNA Contig 176 375 755 Commelina diffusa PPOX_B50_2 gDNA Contig 176 375 756 Commelina diffusa PPOX_B50_3 gDNA Contig 351 550 757 Commelina diffusa PPOX_B50_3 gDNA Contig 351 550 758 Commelina diffusa PPOX_B50_4 gDNA Contig 526 725 759 Commelina diffusa PPOX_B50_4 gDNA Contig 526 725 760 Commelina diffusa PPOX_B50_5 gDNA Contig 701 900 761 Commelina diffusa PPOX_B50_5 gDNA Contig 701 900 762 Commelina diffusa PPOX_B50_6 gDNA Contig 876 1075 763 Commelina diffusa PPOX_B50_6 gDNA Contig 876 1075 764 Commelina diffusa PPOX_B50_7 gDNA Contig 1051 1250 765 Commelina diffusa PPOX_B50_7 gDNA Contig 1051 1250 766 Commelina diffusa PPOX_B50_8 gDNA Contig 1226 1425 767 Commelina diffusa PPOX_B50_8 gDNA Contig 1226 1425 768 Commelina diffusa PPOX_B50_9 gDNA Contig 1401 1600 769 Commelina diffusa PPOX_B50_9 gDNA Contig 1401 1600 770 Commelina diffusa PPOX_B50_10 gDNA Contig 1576 1775 771 Commelina diffusa PPOX_B50_10 gDNA Contig 1576 1775 772 Commelina diffusa PPOX_B50_11 gDNA Contig 1751 1950 773 Commelina diffusa PPOX_B50_11 gDNA Contig 1751 1950 774 Commelina diffusa PPOX_B50_12 gDNA Contig 1926 2125 775 Commelina diffusa PPOX_B50_12 gDNA Contig 1926 2125 776 Commelina diffusa PPOX_B50_13 gDNA Contig 2101 2300 777 Commelina diffusa PPOX_B50_13 gDNA Contig 2101 2300 778 Commelina diffusa PPOX_B50_14 gDNA Contig 2276 2475 779 Commelina diffusa PPOX_B50_14 gDNA Contig 2276 2475 780 Commelina diffusa PPOX_B50_15 gDNA Contig 2451 2650 781 Commelina diffusa PPOX_B50_15 gDNA Contig 2451 2650 782 Commelina diffusa PPOX_B50_16 gDNA Contig 2626 2825 783 Commelina diffusa PPOX_B50_16 gDNA Contig 2626 2825 784 Commelina diffusa PPOX_B50_17 gDNA Contig 2801 3000 785 Commelina diffusa PPOX_B50_17 gDNA Contig 2801 3000 786 Commelina diffusa PPOX_B51_1 gDNA Contig 1 200 787 Commelina diffusa PPOX_B51_1 gDNA Contig 1 200 788 Commelina diffusa PPOX_B51_2 gDNA Contig 176 375 789 Commelina diffusa PPOX_B51_2 gDNA Contig 176 375 790 Commelina diffusa PPOX_B51_3 gDNA Contig 351 550 791 Commelina diffusa PPOX_B51_3 gDNA Contig 351 550 792 Commelina diffusa PPOX_B51_4 gDNA Contig 526 725 793 Commelina diffusa PPOX_B51_4 gDNA Contig 526 725 794 Commelina diffusa PPOX_B51_5 gDNA Contig 701 900 795 Commelina diffusa PPOX_B51_5 gDNA Contig 701 900 796 Commelina diffusa PPOX_B51_6 gDNA Contig 876 1075 797 Commelina diffusa PPOX_B51_6 gDNA Contig 876 1075 798 Commelina diffusa PPOX_B51_7 gDNA Contig 1051 1250 799 Commelina diffusa PPOX_B51_7 gDNA Contig 1051 1250 800 Commelina diffusa PPOX_B51_8 gDNA Contig 1226 1425 801 Commelina diffusa PPOX_B51_8 gDNA Contig 1226 1425 802 Commelina diffusa PPOX_B49_1 cDNA Contig 1 200 803 Commelina diffusa PPOX_B49_1 cDNA Contig 1 200 804 Commelina diffusa PPOX_B49_2 cDNA Contig 176 375 805 Commelina diffusa PPOX_B49_2 cDNA Contig 176 375 806 Commelina diffusa PPOX_B52_1 gDNA Contig 1 200 807 Commelina diffusa PPOX_B52_1 gDNA Contig 1 200 808 Commelina diffusa PPOX_B52_2 gDNA Contig 176 375 809 Commelina diffusa PPOX_B52_2 gDNA Contig 176 375 810 Commelina diffusa PPOX_B52_3 gDNA Contig 351 550 811 Commelina diffusa PPOX_B52_3 gDNA Contig 351 550 812 Conyza canadensis PPOX_B38_1 gDNA Contig 1 200 813 Conyza canadensis PPOX_B38_1 gDNA Contig 1 200 814 Conyza canadensis PPOX_B38_2 gDNA Contig 176 375 815 Conyza canadensis PPOX_B38_2 gDNA Contig 176 375 816 Conyza canadensis PPOX_B38_3 gDNA Contig 351 550 817 Conyza canadensis PPOX_B38_3 gDNA Contig 351 550 818 Conyza canadensis PPOX_B38_4 gDNA Contig 526 725 819 Conyza canadensis PPOX_B38_4 gDNA Contig 526 725 820 Conyza canadensis PPOX_B38_5 gDNA Contig 701 900 821 Conyza canadensis PPOX_B38_5 gDNA Contig 701 900 822 Conyza canadensis PPOX_B38_6 gDNA Contig 876 1075 823 Conyza canadensis PPOX_B38_6 gDNA Contig 876 1075 824 Conyza canadensis PPOX_B38_7 gDNA Contig 1051 1250 825 Conyza canadensis PPOX_B38_7 gDNA Contig 1051 1250 826 Conyza canadensis PPOX_B38_8 gDNA Contig 1226 1425 827 Conyza canadensis PPOX_B38_8 gDNA Contig 1226 1425 828 Conyza canadensis PPOX_B38_9 gDNA Contig 1401 1600 829 Conyza canadensis PPOX_B38_9 gDNA Contig 1401 1600 830 Conyza canadensis PPOX_B38_10 gDNA Contig 1576 1775 831 Conyza canadensis PPOX_B38_10 gDNA Contig 1576 1775 832 Conyza canadensis PPOX_B38_11 gDNA Contig 1751 1950 833 Conyza canadensis PPOX_B38_11 gDNA Contig 1751 1950 834 Conyza canadensis PPOX_B38_12 gDNA Contig 1926 2125 835 Conyza canadensis PPOX_B38_12 gDNA Contig 1926 2125 836 Conyza canadensis PPOX_B38_13 gDNA Contig 2101 2300 837 Conyza canadensis PPOX_B38_13 gDNA Contig 2101 2300 838 Conyza canadensis PPOX_B38_14 gDNA Contig 2276 2475 839 Conyza canadensis PPOX_B38_14 gDNA Contig 2276 2475 840 Conyza canadensis PPOX_B38_15 gDNA Contig 2451 2650 841 Conyza canadensis PPOX_B38_15 gDNA Contig 2451 2650 842 Conyza canadensis PPOX_B38_16 gDNA Contig 2626 2825 843 Conyza canadensis PPOX_B38_16 gDNA Contig 2626 2825 844 Conyza canadensis PPOX_B38_17 gDNA Contig 2801 3000 845 Conyza canadensis PPOX_B38_17 gDNA Contig 2801 3000 846 Conyza canadensis PPOX_B38_18 gDNA Contig 2976 3175 847 Conyza canadensis PPOX_B38_18 gDNA Contig 2976 3175 848 Conyza canadensis PPOX_B38_19 gDNA Contig 3151 3350 849 Conyza canadensis PPOX_B38_19 gDNA Contig 3151 3350 850 Conyza canadensis PPOX_B38_20 gDNA Contig 3326 3525 851 Conyza canadensis PPOX_B38_20 gDNA Contig 3326 3525 852 Conyza canadensis PPOX_B38_21 gDNA Contig 3501 3700 853 Conyza canadensis PPOX_B38_21 gDNA Contig 3501 3700 854 Conyza canadensis PPOX_B38_22 gDNA Contig 3676 3875 855 Conyza canadensis PPOX_B38_22 gDNA Contig 3676 3875 856 Conyza canadensis PPOX_B38_23 gDNA Contig 3851 4050 857 Conyza canadensis PPOX_B38_23 gDNA Contig 3851 4050 858 Conyza canadensis PPOX_B38_24 gDNA Contig 4026 4225 859 Conyza canadensis PPOX_B38_24 gDNA Contig 4026 4225 860 Conyza canadensis PPOX_B38_25 gDNA Contig 4201 4400 861 Conyza canadensis PPOX_B38_25 gDNA Contig 4201 4400 862 Conyza canadensis PPOX_B38_26 gDNA Contig 4376 4575 863 Conyza canadensis PPOX_B38_26 gDNA Contig 4376 4575 864 Conyza canadensis PPOX_B38_27 gDNA Contig 4551 4750 865 Conyza canadensis PPOX_B38_27 gDNA Contig 4551 4750 866 Conyza canadensis PPOX_B38_28 gDNA Contig 4726 4925 867 Conyza canadensis PPOX_B38_28 gDNA Contig 4726 4925 868 Conyza canadensis PPOX_B38_29 gDNA Contig 4901 5100 869 Conyza canadensis PPOX_B38_29 gDNA Contig 4901 5100 870 Conyza canadensis PPOX_B38_30 gDNA Contig 5076 5275 871 Conyza canadensis PPOX_B38_30 gDNA Contig 5076 5275 872 Conyza canadensis PPOX_B38_31 gDNA Contig 5251 5450 873 Conyza canadensis PPOX_B38_31 gDNA Contig 5251 5450 874 Conyza canadensis PPOX_B38_32 gDNA Contig 5426 5625 875 Conyza canadensis PPOX_B38_32 gDNA Contig 5426 5625 876 Conyza canadensis PPOX_B38_33 gDNA Contig 5601 5800 877 Conyza canadensis PPOX_B38_33 gDNA Contig 5601 5800 878 Conyza canadensis PPOX_B38_34 gDNA Contig 5776 5975 879 Conyza canadensis PPOX_B38_34 gDNA Contig 5776 5975 880 Conyza canadensis PPOX_B38_35 gDNA Contig 5951 6150 881 Conyza canadensis PPOX_B38_35 gDNA Contig 5951 6150 882 Conyza canadensis PPOX_B38_36 gDNA Contig 6126 6325 883 Conyza canadensis PPOX_B38_36 gDNA Contig 6126 6325 884 Conyza canadensis PPOX_B38_37 gDNA Contig 6301 6500 885 Conyza canadensis PPOX_B38_37 gDNA Contig 6301 6500 886 Conyza canadensis PPOX_B38_38 gDNA Contig 6476 6675 887 Conyza canadensis PPOX_B38_38 gDNA Contig 6476 6675 888 Conyza canadensis PPOX_B38_39 gDNA Contig 6651 6850 889 Conyza canadensis PPOX_B38_39 gDNA Contig 6651 6850 890 Conyza canadensis PPOX_B38_40 gDNA Contig 6826 7025 891 Conyza canadensis PPOX_B38_40 gDNA Contig 6826 7025 892 Conyza canadensis PPOX_B38_41 gDNA Contig 7001 7200 893 Conyza canadensis PPOX_B38_41 gDNA Contig 7001 7200 894 Conyza canadensis PPOX_B38_42 gDNA Contig 7176 7375 895 Conyza canadensis PPOX_B38_42 gDNA Contig 7176 7375 896 Conyza canadensis PPOX_B38_43 gDNA Contig 7351 7550 897 Conyza canadensis PPOX_B38_43 gDNA Contig 7351 7550 898 Conyza canadensis PPOX_B38_44 gDNA Contig 7526 7725 899 Conyza canadensis PPOX_B38_44 gDNA Contig 7526 7725 900 Conyza canadensis PPOX_B38_45 gDNA Contig 7701 7900 901 Conyza canadensis PPOX_B38_45 gDNA Contig 7701 7900 902 Conyza canadensis PPOX_B38_46 gDNA Contig 7876 8075 903 Conyza canadensis PPOX_B38_46 gDNA Contig 7876 8075 904 Conyza canadensis PPOX_B38_47 gDNA Contig 8051 8250 905 Conyza canadensis PPOX_B38_47 gDNA Contig 8051 8250 906 Conyza canadensis PPOX_B38_48 gDNA Contig 8226 8425 907 Conyza canadensis PPOX_B38_48 gDNA Contig 8226 8425 908 Conyza canadensis PPOX_B38_49 gDNA Contig 8401 8600 909 Conyza canadensis PPOX_B38_49 gDNA Contig 8401 8600 910 Conyza canadensis PPOX_B38_50 gDNA Contig 8576 8775 911 Conyza canadensis PPOX_B38_50 gDNA Contig 8576 8775 912 Conyza canadensis PPOX_B38_51 gDNA Contig 8751 8950 913 Conyza canadensis PPOX_B38_51 gDNA Contig 8751 8950 914 Conyza canadensis PPOX_B38_52 gDNA Contig 8926 9125 915 Conyza canadensis PPOX_B38_52 gDNA Contig 8926 9125 916 Conyza canadensis PPOX_B38_53 gDNA Contig 9101 9300 917 Conyza canadensis PPOX_B38_53 gDNA Contig 9101 9300 918 Conyza canadensis PPOX_B38_54 gDNA Contig 9276 9475 919 Conyza canadensis PPOX_B38_54 gDNA Contig 9276 9475 920 Conyza canadensis PPOX_B38_55 gDNA Contig 9451 9650 921 Conyza canadensis PPOX_B38_55 gDNA Contig 9451 9650 922 Conyza canadensis PPOX_B38_56 gDNA Contig 9626 9825 923 Conyza canadensis PPOX_B38_56 gDNA Contig 9626 9825 924 Conyza canadensis PPOX_B38_57 gDNA Contig 9801 10000 925 Conyza canadensis PPOX_B38_57 gDNA Contig 9801 10000 926 Conyza canadensis PPOX_B38_58 gDNA Contig 9976 10175 927 Conyza canadensis PPOX_B38_58 gDNA Contig 9976 10175 928 Conyza canadensis PPOX_B38_59 gDNA Contig 10151 10350 929 Conyza canadensis PPOX_B38_59 gDNA Contig 10151 10350 930 Conyza canadensis PPOX_B38_60 gDNA Contig 10326 10525 931 Conyza canadensis PPOX_B38_60 gDNA Contig 10326 10525 932 Conyza canadensis PPOX_B39_1 gDNA Contig 1 200 933 Conyza canadensis PPOX_B39_1 gDNA Contig 1 200 934 Conyza canadensis PPOX_B39_2 gDNA Contig 176 375 935 Conyza canadensis PPOX_B39_2 gDNA Contig 176 375 936 Conyza canadensis PPOX_B39_3 gDNA Contig 351 550 937 Conyza canadensis PPOX_B39_3 gDNA Contig 351 550 938 Conyza canadensis PPOX_B39_4 gDNA Contig 526 725 939 Conyza canadensis PPOX_B39_4 gDNA Contig 526 725 940 Conyza canadensis PPOX_B39_5 gDNA Contig 701 900 941 Conyza canadensis PPOX_B39_5 gDNA Contig 701 900 942 Conyza canadensis PPOX_B39_6 gDNA Contig 876 1075 943 Conyza canadensis PPOX_B39_6 gDNA Contig 876 1075 944 Conyza canadensis PPOX_B39_7 gDNA Contig 1051 1250 945 Conyza canadensis PPOX_B39_7 gDNA Contig 1051 1250 946 Conyza canadensis PPOX_B39_8 gDNA Contig 1226 1425 947 Conyza canadensis PPOX_B39_8 gDNA Contig 1226 1425 948 Conyza canadensis PPOX_B39_9 gDNA Contig 1401 1600 949 Conyza canadensis PPOX_B39_9 gDNA Contig 1401 1600 950 Conyza canadensis PPOX_B39_10 gDNA Contig 1576 1775 951 Conyza canadensis PPOX_B39_10 gDNA Contig 1576 1775 952 Conyza canadensis PPOX_B39_11 gDNA Contig 1751 1950 953 Conyza canadensis PPOX_B39_11 gDNA Contig 1751 1950 954 Conyza canadensis PPOX_B39_12 gDNA Contig 1926 2125 955 Conyza canadensis PPOX_B39_12 gDNA Contig 1926 2125 956 Conyza canadensis PPOX_B39_13 gDNA Contig 2101 2300 957 Conyza canadensis PPOX_B39_13 gDNA Contig 2101 2300 958 Conyza canadensis PPOX_B39_14 gDNA Contig 2276 2475 959 Conyza canadensis PPOX_B39_14 gDNA Contig 2276 2475 960 Conyza canadensis PPOX_B39_15 gDNA Contig 2451 2650 961 Conyza canadensis PPOX_B39_15 gDNA Contig 2451 2650 962 Conyza canadensis PPOX_B39_16 gDNA Contig 2626 2825 963 Conyza canadensis PPOX_B39_16 gDNA Contig 2626 2825 964 Conyza canadensis PPOX_B39_17 gDNA Contig 2801 3000 965 Conyza canadensis PPOX_B39_17 gDNA Contig 2801 3000 966 Conyza canadensis PPOX_B39_18 gDNA Contig 2976 3175 967 Conyza canadensis PPOX_B39_18 gDNA Contig 2976 3175 968 Conyza canadensis PPOX_B39_19 gDNA Contig 3151 3350 969 Conyza canadensis PPOX_B39_19 gDNA Contig 3151 3350 970 Conyza canadensis PPOX_B39_20 gDNA Contig 3326 3525 971 Conyza canadensis PPOX_B39_20 gDNA Contig 3326 3525 972 Conyza canadensis PPOX_B39_21 gDNA Contig 3501 3700 973 Conyza canadensis PPOX_B39_21 gDNA Contig 3501 3700 974 Conyza canadensis PPOX_B39_22 gDNA Contig 3676 3875 975 Conyza canadensis PPOX_B39_22 gDNA Contig 3676 3875 976 Conyza canadensis PPOX_B39_23 gDNA Contig 3851 4050 977 Conyza canadensis PPOX_B39_23 gDNA Contig 3851 4050 978 Conyza canadensis PPOX_B39_24 gDNA Contig 4026 4225 979 Conyza canadensis PPOX_B39_24 gDNA Contig 4026 4225 980 Conyza canadensis PPOX_B39_25 gDNA Contig 4201 4400 981 Conyza canadensis PPOX_B39_25 gDNA Contig 4201 4400 982 Conyza canadensis PPOX_B39_26 gDNA Contig 4376 4575 983 Conyza canadensis PPOX_B39_26 gDNA Contig 4376 4575 984 Conyza canadensis PPOX_B39_27 gDNA Contig 4551 4750 985 Conyza canadensis PPOX_B39_27 gDNA Contig 4551 4750 986 Conyza canadensis PPOX_B39_28 gDNA Contig 4726 4925 987 Conyza canadensis PPOX_B39_28 gDNA Contig 4726 4925 988 Conyza canadensis PPOX_B39_29 gDNA Contig 4901 5100 989 Conyza canadensis PPOX_B39_29 gDNA Contig 4901 5100 990 Conyza canadensis PPOX_B39_30 gDNA Contig 5076 5275 991 Conyza canadensis PPOX_B39_30 gDNA Contig 5076 5275 992 Conyza canadensis PPOX_B39_31 gDNA Contig 5251 5450 993 Conyza canadensis PPOX_B39_31 gDNA Contig 5251 5450 994 Conyza canadensis PPOX_B39_32 gDNA Contig 5426 5625 995 Conyza canadensis PPOX_B39_32 gDNA Contig 5426 5625 996 Conyza canadensis PPOX_B39_33 gDNA Contig 5601 5800 997 Conyza canadensis PPOX_B39_33 gDNA Contig 5601 5800 998 Conyza canadensis PPOX_B39_34 gDNA Contig 5776 5975 999 Conyza canadensis PPOX_B39_34 gDNA Contig 5776 5975 1000 Conyza canadensis PPOX_B39_35 gDNA Contig 5951 6150 1001 Conyza canadensis PPOX_B39_35 gDNA Contig 5951 6150 1002 Conyza canadensis PPOX_B39_36 gDNA Contig 6126 6325 1003 Conyza canadensis PPOX_B39_36 gDNA Contig 6126 6325 1004 Conyza canadensis PPOX_B39_37 gDNA Contig 6301 6500 1005 Conyza canadensis PPOX_B39_37 gDNA Contig 6301 6500 1006 Conyza canadensis PPOX_B39_38 gDNA Contig 6476 6675 1007 Conyza canadensis PPOX_B39_38 gDNA Contig 6476 6675 1008 Conyza canadensis PPOX_B39_39 gDNA Contig 6651 6850 1009 Conyza canadensis PPOX_B39_39 gDNA Contig 6651 6850 1010 Conyza canadensis PPOX_B39_40 gDNA Contig 6826 7025 1011 Conyza canadensis PPOX_B39_40 gDNA Contig 6826 7025 1012 Conyza canadensis PPOX_B39_41 gDNA Contig 7001 7200 1013 Conyza canadensis PPOX_B39_41 gDNA Contig 7001 7200 1014 Conyza canadensis PPOX_B39_42 gDNA Contig 7176 7375 1015 Conyza canadensis PPOX_B39_42 gDNA Contig 7176 7375 1016 Conyza canadensis PPOX_B39_43 gDNA Contig 7351 7550 1017 Conyza canadensis PPOX_B39_43 gDNA Contig 7351 7550 1018 Conyza canadensis PPOX_B39_44 gDNA Contig 7526 7725 1019 Conyza canadensis PPOX_B39_44 gDNA Contig 7526 7725 1020 Conyza canadensis PPOX_B39_45 gDNA Contig 7701 7900 1021 Conyza canadensis PPOX_B39_45 gDNA Contig 7701 7900 1022 Conyza canadensis PPOX_B39_46 gDNA Contig 7876 8075 1023 Conyza canadensis PPOX_B39_46 gDNA Contig 7876 8075 1024 Conyza canadensis PPOX_B39_47 gDNA Contig 8051 8250 1025 Conyza canadensis PPOX_B39_47 gDNA Contig 8051 8250 1026 Conyza canadensis PPOX_B39_48 gDNA Contig 8226 8425 1027 Conyza canadensis PPOX_B39_48 gDNA Contig 8226 8425 1028 Conyza canadensis PPOX_B39_49 gDNA Contig 8401 8600 1029 Conyza canadensis PPOX_B39_49 gDNA Contig 8401 8600 1030 Conyza canadensis PPOX_B39_50 gDNA Contig 8576 8775 1031 Conyza canadensis PPOX_B39_50 gDNA Contig 8576 8775 1032 Conyza canadensis PPOX_B39_51 gDNA Contig 8751 8950 1033 Conyza canadensis PPOX_B39_51 gDNA Contig 8751 8950 1034 Conyza canadensis PPOX_B39_52 gDNA Contig 8926 9125 1035 Conyza canadensis PPOX_B39_52 gDNA Contig 8926 9125 1036 Conyza canadensis PPOX_B39_53 gDNA Contig 9101 9300 1037 Conyza canadensis PPOX_B39_53 gDNA Contig 9101 9300 1038 Conyza canadensis PPOX_B39_54 gDNA Contig 9276 9475 1039 Conyza canadensis PPOX_B39_54 gDNA Contig 9276 9475 1040 Conyza canadensis PPOX_B39_55 gDNA Contig 9451 9650 1041 Conyza canadensis PPOX_B39_55 gDNA Contig 9451 9650 1042 Conyza canadensis PPOX_B39_56 gDNA Contig 9626 9825 1043 Conyza canadensis PPOX_B39_56 gDNA Contig 9626 9825 1044 Conyza canadensis PPOX_B39_57 gDNA Contig 9801 10000 1045 Conyza canadensis PPOX_B39_57 gDNA Contig 9801 10000 1046 Conyza canadensis PPOX_B39_58 gDNA Contig 9976 10175 1047 Conyza canadensis PPOX_B39_58 gDNA Contig 9976 10175 1048 Conyza canadensis PPOX_B36_1 cDNA Contig 1 200 1049 Conyza canadensis PPOX_B36_1 cDNA Contig 1 200 1050 Conyza canadensis PPOX_B36_2 cDNA Contig 176 375 1051 Conyza canadensis PPOX_B36_2 cDNA Contig 176 375 1052 Conyza canadensis PPOX_B36_3 cDNA Contig 351 550 1053 Conyza canadensis PPOX_B36_3 cDNA Contig 351 550 1054 Conyza canadensis PPOX_B36_4 cDNA Contig 526 725 1055 Conyza canadensis PPOX_B36_4 cDNA Contig 526 725 1056 Conyza canadensis PPOX_B36_5 cDNA Contig 701 900 1057 Conyza canadensis PPOX_B36_5 cDNA Contig 701 900 1058 Conyza canadensis PPOX_B36_6 cDNA Contig 876 1075 1059 Conyza canadensis PPOX_B36_6 cDNA Contig 876 1075 1060 Conyza canadensis PPOX_B36_7 cDNA Contig 1051 1250 1061 Conyza canadensis PPOX_B36_7 cDNA Contig 1051 1250 1062 Conyza canadensis PPOX_B36_8 cDNA Contig 1226 1425 1063 Conyza canadensis PPOX_B36_8 cDNA Contig 1226 1425 1064 Conyza canadensis PPOX_B36_9 cDNA Contig 1401 1600 1065 Conyza canadensis PPOX_B36_9 cDNA Contig 1401 1600 1066 Conyza canadensis PPOX_B40_1 gDNA Contig 1 200 1067 Conyza canadensis PPOX_B40_1 gDNA Contig 1 200 1068 Conyza canadensis PPOX_B40_2 gDNA Contig 176 375 1069 Conyza canadensis PPOX_B40_2 gDNA Contig 176 375 1070 Conyza canadensis PPOX_B40_3 gDNA Contig 351 550 1071 Conyza canadensis PPOX_B40_3 gDNA Contig 351 550 1072 Conyza canadensis PPOX_B40_4 gDNA Contig 526 725 1073 Conyza canadensis PPOX_B40_4 gDNA Contig 526 725 1074 Conyza canadensis PPOX_B40_5 gDNA Contig 701 900 1075 Conyza canadensis PPOX_B40_5 gDNA Contig 701 900 1076 Conyza canadensis PPOX_B40_6 gDNA Contig 876 1075 1077 Conyza canadensis PPOX_B40_6 gDNA Contig 876 1075 1078 Conyza canadensis PPOX_B40_7 gDNA Contig 1051 1250 1079 Conyza canadensis PPOX_B40_7 gDNA Contig 1051 1250 1080 Conyza canadensis PPOX_B40_8 gDNA Contig 1226 1425 1081 Conyza canadensis PPOX_B40_8 gDNA Contig 1226 1425 1082 Conyza canadensis PPOX_B40_9 gDNA Contig 1401 1600 1083 Conyza canadensis PPOX_B40_9 gDNA Contig 1401 1600 1084 Conyza canadensis PPOX_B40_10 gDNA Contig 1576 1775 1085 Conyza canadensis PPOX_B40_10 gDNA Contig 1576 1775 1086 Conyza canadensis PPOX_B40_11 gDNA Contig 1751 1950 1087 Conyza canadensis PPOX_B40_11 gDNA Contig 1751 1950 1088 Conyza canadensis PPOX_B40_12 gDNA Contig 1926 2125 1089 Conyza canadensis PPOX_B40_12 gDNA Contig 1926 2125 1090 Conyza canadensis PPOX_B40_13 gDNA Contig 2101 2300 1091 Conyza canadensis PPOX_B40_13 gDNA Contig 2101 2300 1092 Conyza canadensis PPOX_B40_14 gDNA Contig 2276 2475 1093 Conyza canadensis PPOX_B40_14 gDNA Contig 2276 2475 1094 Conyza canadensis PPOX_B37_1 cDNA Contig 1 200 1095 Conyza canadensis PPOX_B37_1 cDNA Contig 1 200 1096 Conyza canadensis PPOX_B37_2 cDNA Contig 176 375 1097 Conyza canadensis PPOX_B37_2 cDNA Contig 176 375 1098 Conyza canadensis PPOX_B37_3 cDNA Contig 351 550 1099 Conyza canadensis PPOX_B37_3 cDNA Contig 351 550 1100 Digitaria sanguinalis PPOX_B54_1 cDNA Contig 1 200 1101 Digitaria sanguinalis PPOX_B54_1 cDNA Contig 1 200 1102 Digitaria sanguinalis PPOX_B54_2 cDNA Contig 176 375 1103 Digitaria sanguinalis PPOX_B54_2 cDNA Contig 176 375 1104 Digitaria sanguinalis PPOX_B56_1 gDNA Contig 1 200 1105 Digitaria sanguinalis PPOX_B56_1 gDNA Contig 1 200 1106 Digitaria sanguinalis PPOX_B56_2 gDNA Contig 176 375 1107 Digitaria sanguinalis PPOX_B56_2 gDNA Contig 176 375 1108 Digitaria sanguinalis PPOX_B56_3 gDNA Contig 351 550 1109 Digitaria sanguinalis PPOX_B56_3 gDNA Contig 351 550 1110 Digitaria sanguinalis PPOX_B56_4 gDNA Contig 526 725 1111 Digitaria sanguinalis PPOX_B56_4 gDNA Contig 526 725 1112 Digitaria sanguinalis PPOX_B56_5 gDNA Contig 701 900 1113 Digitaria sanguinalis PPOX_B56_5 gDNA Contig 701 900 1114 Digitaria sanguinalis PPOX_B56_6 gDNA Contig 876 1075 1115 Digitaria sanguinalis PPOX_B56_6 gDNA Contig 876 1075 1116 Digitaria sanguinalis PPOX_B56_7 gDNA Contig 1051 1250 1117 Digitaria sanguinalis PPOX_B56_7 gDNA Contig 1051 1250 1118 Digitaria sanguinalis PPOX_B56_8 gDNA Contig 1226 1425 1119 Digitaria sanguinalis PPOX_B56_8 gDNA Contig 1226 1425 1120 Digitaria sanguinalis PPOX_B56_9 gDNA Contig 1401 1600 1121 Digitaria sanguinalis PPOX_B56_9 gDNA Contig 1401 1600 1122 Digitaria sanguinalis PPOX_B56_10 gDNA Contig 1576 1775 1123 Digitaria sanguinalis PPOX_B56_10 gDNA Contig 1576 1775 1124 Digitaria sanguinalis PPOX_B56_11 gDNA Contig 1751 1950 1125 Digitaria sanguinalis PPOX_B56_11 gDNA Contig 1751 1950 1126 Digitaria sanguinalis PPOX_B56_12 gDNA Contig 1926 2125 1127 Digitaria sanguinalis PPOX_B56_12 gDNA Contig 1926 2125 1128 Digitaria sanguinalis PPOX_B56_13 gDNA Contig 2101 2300 1129 Digitaria sanguinalis PPOX_B56_13 gDNA Contig 2101 2300 1130 Digitaria sanguinalis PPOX_B56_14 gDNA Contig 2276 2475 1131 Digitaria sanguinalis PPOX_B56_14 gDNA Contig 2276 2475 1132 Digitaria sanguinalis PPOX_B56_15 gDNA Contig 2451 2650 1133 Digitaria sanguinalis PPOX_B56_15 gDNA Contig 2451 2650 1134 Digitaria sanguinalis PPOX_B53_1 cDNA Contig 1 200 1135 Digitaria sanguinalis PPOX_B53_1 cDNA Contig 1 200 1136 Digitaria sanguinalis PPOX_B53_2 cDNA Contig 176 375 1137 Digitaria sanguinalis PPOX_B53_2 cDNA Contig 176 375 1138 Digitaria sanguinalis PPOX_B53_3 cDNA Contig 351 550 1139 Digitaria sanguinalis PPOX_B53_3 cDNA Contig 351 550 1140 Digitaria sanguinalis PPOX_B53_4 cDNA Contig 526 725 1141 Digitaria sanguinalis PPOX_B53_4 cDNA Contig 526 725 1142 Digitaria sanguinalis PPOX_B57_1 gDNA Contig 1 200 1143 Digitaria sanguinalis PPOX_B57_1 gDNA Contig 1 200 1144 Digitaria sanguinalis PPOX_B57_2 gDNA Contig 176 375 1145 Digitaria sanguinalis PPOX_B57_2 gDNA Contig 176 375 1146 Digitaria sanguinalis PPOX_B57_3 gDNA Contig 351 550 1147 Digitaria sanguinalis PPOX_B57_3 gDNA Contig 351 550 1148 Digitaria sanguinalis PPOX_B57_4 gDNA Contig 526 725 1149 Digitaria sanguinalis PPOX_B57_4 gDNA Contig 526 725 1150 Digitaria sanguinalis PPOX_B57_5 gDNA Contig 701 900 1151 Digitaria sanguinalis PPOX_B57_5 gDNA Contig 701 900 1152 Digitaria sanguinalis PPOX_B57_6 gDNA Contig 876 1075 1153 Digitaria sanguinalis PPOX_B57_6 gDNA Contig 876 1075 1154 Digitaria sanguinalis PPOX_B55_1 cDNA Contig 1 200 1155 Digitaria sanguinalis PPOX_B55_1 cDNA Contig 1 200 1156 Digitaria sanguinalis PPOX_B55_2 cDNA Contig 176 375 1157 Digitaria sanguinalis PPOX_B55_2 cDNA Contig 176 375 1158 Euphorbia heterophylla PPOX_B47_1 gDNA Contig 1 121 1159 Euphorbia heterophylla PPOX_B47_1 gDNA Contig 1 121 1160 Euphorbia heterophylla PPOX_B42_1 cDNA Contig 1 200 1161 Euphorbia heterophylla PPOX_B42_1 cDNA Contig 1 200 1162 Euphorbia heterophylla PPOX_B42_2 cDNA Contig 176 375 1163 Euphorbia heterophylla PPOX_B42_2 cDNA Contig 176 375 1164 Euphorbia heterophylla PPOX_B46_1 gDNA Contig 1 200 1165 Euphorbia heterophylla PPOX_B46_1 gDNA Contig 1 200 1166 Euphorbia heterophylla PPOX_B46_2 gDNA Contig 176 375 1167 Euphorbia heterophylla PPOX_B46_2 gDNA Contig 176 375 1168 Euphorbia heterophylla PPOX_B46_3 gDNA Contig 351 550 1169 Euphorbia heterophylla PPOX_B46_3 gDNA Contig 351 550 1170 Euphorbia heterophylla PPOX_B46_4 gDNA Contig 526 725 1171 Euphorbia heterophylla PPOX_B46_4 gDNA Contig 526 725 1172 Euphorbia heterophylla PPOX_B45_1 gDNA Contig 1 200 1173 Euphorbia heterophylla PPOX_B45_1 gDNA Contig 1 200 1174 Euphorbia heterophylla PPOX_B45_2 gDNA Contig 176 375 1175 Euphorbia heterophylla PPOX_B45_2 gDNA Contig 176 375 1176 Euphorbia heterophylla PPOX_B45_3 gDNA Contig 351 550 1177 Euphorbia heterophylla PPOX_B45_3 gDNA Contig 351 550 1178 Euphorbia heterophylla PPOX_B45_4 gDNA Contig 526 725 1179 Euphorbia heterophylla PPOX_B45_4 gDNA Contig 526 725 1180 Euphorbia heterophylla PPOX_B45_5 gDNA Contig 701 900 1181 Euphorbia heterophylla PPOX_B45_5 gDNA Contig 701 900 1182 Euphorbia heterophylla PPOX_B41_1 cDNA Contig 1 200 1183 Euphorbia heterophylla PPOX_B41_1 cDNA Contig 1 200 1184 Euphorbia heterophylla PPOX_B41_2 cDNA Contig 176 375 1185 Euphorbia heterophylla PPOX_B41_2 cDNA Contig 176 375 1186 Euphorbia heterophylla PPOX_B41_3 cDNA Contig 351 550 1187 Euphorbia heterophylla PPOX_B41_3 cDNA Contig 351 550 1188 Euphorbia heterophylla PPOX_B41_4 cDNA Contig 526 725 1189 Euphorbia heterophylla PPOX_B41_4 cDNA Contig 526 725 1190 Euphorbia heterophylla PPOX_B41_5 cDNA Contig 701 900 1191 Euphorbia heterophylla PPOX_B41_5 cDNA Contig 701 900 1192 Euphorbia heterophylla PPOX_B41_6 cDNA Contig 876 1075 1193 Euphorbia heterophylla PPOX_B41_6 cDNA Contig 876 1075 1194 Euphorbia heterophylla PPOX_B41_7 cDNA Contig 1051 1250 1195 Euphorbia heterophylla PPOX_B41_7 cDNA Contig 1051 1250 1196 Euphorbia heterophylla PPOX_B41_8 cDNA Contig 1226 1425 1197 Euphorbia heterophylla PPOX_B41_8 cDNA Contig 1226 1425 1198 Euphorbia heterophylla PPOX_B41_9 cDNA Contig 1401 1600 1199 Euphorbia heterophylla PPOX_B41_9 cDNA Contig 1401 1600 1200 Euphorbia heterophylla PPOX_B44_1 gDNA Contig 1 200 1201 Euphorbia heterophylla PPOX_B44_1 gDNA Contig 1 200 1202 Euphorbia heterophylla PPOX_B44_2 gDNA Contig 176 375 1203 Euphorbia heterophylla PPOX_B44_2 gDNA Contig 176 375 1204 Euphorbia heterophylla PPOX_B44_3 gDNA Contig 351 550 1205 Euphorbia heterophylla PPOX_B44_3 gDNA Contig 351 550 1206 Euphorbia heterophylla PPOX_B44_4 gDNA Contig 526 725 1207 Euphorbia heterophylla PPOX_B44_4 gDNA Contig 526 725 1208 Euphorbia heterophylla PPOX_B44_5 gDNA Contig 701 900 1209 Euphorbia heterophylla PPOX_B44_5 gDNA Contig 701 900 1210 Euphorbia heterophylla PPOX_B44_6 gDNA Contig 876 1075 1211 Euphorbia heterophylla PPOX_B44_6 gDNA Contig 876 1075 1212 Euphorbia heterophylla PPOX_B44_7 gDNA Contig 1051 1250 1213 Euphorbia heterophylla PPOX_B44_7 gDNA Contig 1051 1250 1214 Euphorbia heterophylla PPOX_B44_8 gDNA Contig 1226 1425 1215 Euphorbia heterophylla PPOX_B44_8 gDNA Contig 1226 1425 1216 Euphorbia heterophylla PPOX_B44_9 gDNA Contig 1401 1600 1217 Euphorbia heterophylla PPOX_B44_9 gDNA Contig 1401 1600 1218 Euphorbia heterophylla PPOX_B44_10 gDNA Contig 1576 1775 1219 Euphorbia heterophylla PPOX_B44_10 gDNA Contig 1576 1775 1220 Euphorbia heterophylla PPOX_B44_11 gDNA Contig 1751 1950 1221 Euphorbia heterophylla PPOX_B44_11 gDNA Contig 1751 1950 1222 Euphorbia heterophylla PPOX_B44_12 gDNA Contig 1926 2125 1223 Euphorbia heterophylla PPOX_B44_12 gDNA Contig 1926 2125 1224 Euphorbia heterophylla PPOX_B44_13 gDNA Contig 2101 2300 1225 Euphorbia heterophylla PPOX_B44_13 gDNA Contig 2101 2300 1226 Euphorbia heterophylla PPOX_B44_14 gDNA Contig 2276 2475 1227 Euphorbia heterophylla PPOX_B44_14 gDNA Contig 2276 2475 1228 Euphorbia heterophylla PPOX_B43_1 gDNA Contig 1 200 1229 Euphorbia heterophylla PPOX_B43_1 gDNA Contig 1 200 1230 Euphorbia heterophylla PPOX_B43_2 gDNA Contig 176 375 1231 Euphorbia heterophylla PPOX_B43_2 gDNA Contig 176 375 1232 Euphorbia heterophylla PPOX_B43_3 gDNA Contig 351 550 1233 Euphorbia heterophylla PPOX_B43_3 gDNA Contig 351 550 1234 Euphorbia heterophylla PPOX_B43_4 gDNA Contig 526 725 1235 Euphorbia heterophylla PPOX_B43_4 gDNA Contig 526 725 1236 Euphorbia heterophylla PPOX_B43_5 gDNA Contig 701 900 1237 Euphorbia heterophylla PPOX_B43_5 gDNA Contig 701 900 1238 Euphorbia heterophylla PPOX_B43_6 gDNA Contig 876 1075 1239 Euphorbia heterophylla PPOX_B43_6 gDNA Contig 876 1075 1240 Euphorbia heterophylla PPOX_B43_7 gDNA Contig 1051 1250 1241 Euphorbia heterophylla PPOX_B43_7 gDNA Contig 1051 1250 1242 Euphorbia heterophylla PPOX_B43_8 gDNA Contig 1226 1425 1243 Euphorbia heterophylla PPOX_B43_8 gDNA Contig 1226 1425 1244 Euphorbia heterophylla PPOX_B43_9 gDNA Contig 1401 1600 1245 Euphorbia heterophylla PPOX_B43_9 gDNA Contig 1401 1600 1246 Euphorbia heterophylla PPOX_B43_10 gDNA Contig 1576 1775 1247 Euphorbia heterophylla PPOX_B43_10 gDNA Contig 1576 1775 1248 Euphorbia heterophylla PPOX_B43_11 gDNA Contig 1751 1950 1249 Euphorbia heterophylla PPOX_B43_11 gDNA Contig 1751 1950 1250 Euphorbia heterophylla PPOX_B43_12 gDNA Contig 1926 2125 1251 Euphorbia heterophylla PPOX_B43_12 gDNA Contig 1926 2125 1252 Euphorbia heterophylla PPOX_B43_13 gDNA Contig 2101 2300 1253 Euphorbia heterophylla PPOX_B43_13 gDNA Contig 2101 2300 1254 Euphorbia heterophylla PPOX_B43_14 gDNA Contig 2276 2475 1255 Euphorbia heterophylla PPOX_B43_14 gDNA Contig 2276 2475 1256 Euphorbia heterophylla PPOX_B43_15 gDNA Contig 2451 2650 1257 Euphorbia heterophylla PPOX_B43_15 gDNA Contig 2451 2650 1258 Euphorbia heterophylla PPOX_B43_16 gDNA Contig 2626 2825 1259 Euphorbia heterophylla PPOX_B43_16 gDNA Contig 2626 2825 1260 Euphorbia heterophylla PPOX_B43_17 gDNA Contig 2801 3000 1261 Euphorbia heterophylla PPOX_B43_17 gDNA Contig 2801 3000 1262 Euphorbia heterophylla PPOX_B43_18 gDNA Contig 2976 3175 1263 Euphorbia heterophylla PPOX_B43_18 gDNA Contig 2976 3175 1264 Euphorbia heterophylla PPOX_B43_19 gDNA Contig 3151 3350 1265 Euphorbia heterophylla PPOX_B43_19 gDNA Contig 3151 3350 1266 Euphorbia heterophylla PPOX_B43_20 gDNA Contig 3326 3525 1267 Euphorbia heterophylla PPOX_B43_20 gDNA Contig 3326 3525 1268 Euphorbia heterophylla PPOX_B43_21 gDNA Contig 3501 3700 1269 Euphorbia heterophylla PPOX_B43_21 gDNA Contig 3501 3700 1270 Euphorbia heterophylla PPOX_B43_22 gDNA Contig 3676 3875 1271 Euphorbia heterophylla PPOX_B43_22 gDNA Contig 3676 3875 1272 Euphorbia heterophylla PPOX_B43_23 gDNA Contig 3851 4050 1273 Euphorbia heterophylla PPOX_B43_23 gDNA Contig 3851 4050 1274 Euphorbia heterophylla PPOX_B48_1 gDNA Contig 1 77 1275 Euphorbia heterophylla PPOX_B48_1 gDNA Contig 1 77 1276 Kochia scoparia PPOX_B58_1 cDNA Contig 1 200 1277 Kochia scoparia PPOX_B58_1 cDNA Contig 1 200 1278 Kochia scoparia PPOX_B58_2 cDNA Contig 176 375 1279 Kochia scoparia PPOX_B58_2 cDNA Contig 176 375 1280 Kochia scoparia PPOX_B58_3 cDNA Contig 351 550 1281 Kochia scoparia PPOX_B58_3 cDNA Contig 351 550 1282 Kochia scoparia PPOX_B58_4 cDNA Contig 526 725 1283 Kochia scoparia PPOX_B58_4 cDNA Contig 526 725 1284 Kochia scoparia PPOX_B58_5 cDNA Contig 701 900 1285 Kochia scoparia PPOX_B58_5 cDNA Contig 701 900 1286 Kochia scoparia PPOX_B58_6 cDNA Contig 876 1075 1287 Kochia scoparia PPOX_B58_6 cDNA Contig 876 1075 1288 Kochia scoparia PPOX_B58_7 cDNA Contig 1051 1250 1289 Kochia scoparia PPOX_B58_7 cDNA Contig 1051 1250 1290 Kochia scoparia PPOX_B58_8 cDNA Contig 1226 1425 1291 Kochia scoparia PPOX_B58_8 cDNA Contig 1226 1425 1292 Kochia scoparia PPOX_B59_1 cDNA Contig 1 200 1293 Kochia scoparia PPOX_B59_1 cDNA Contig 1 200 1294 Kochia scoparia PPOX_B59_2 cDNA Contig 176 375 1295 Kochia scoparia PPOX_B59_2 cDNA Contig 176 375 1296 Kochia scoparia PPOX_B59_3 cDNA Contig 351 550 1297 Kochia scoparia PPOX_B59_3 cDNA Contig 351 550 1298 Kochia scoparia PPOX_B59_4 cDNA Contig 526 725 1299 Kochia scoparia PPOX_B59_4 cDNA Contig 526 725 1300 Kochia scoparia PPOX_B59_5 cDNA Contig 701 900 1301 Kochia scoparia PPOX_B59_5 cDNA Contig 701 900 1302 Kochia scoparia PPOX_B60_1 cDNA Contig 1 200 1303 Kochia scoparia PPOX_B60_1 cDNA Contig 1 200 1304 Kochia scoparia PPOX_B60_2 cDNA Contig 176 375 1305 Kochia scoparia PPOX_B60_2 cDNA Contig 176 375 1306 Kochia scoparia PPOX_B60_3 cDNA Contig 351 550 1307 Kochia scoparia PPOX_B60_3 cDNA Contig 351 550 1308 Kochia scoparia PPOX_B61_1 cDNA Contig 1 200 1309 Kochia scoparia PPOX_B61_1 cDNA Contig 1 200 1310 Kochia scoparia PPOX_B61_2 cDNA Contig 176 375 1311 Kochia scoparia PPOX_B61_2 cDNA Contig 176 375 1312 Lolium multiflorum PPOX_B65_1 cDNA Contig 1 150 1313 Lolium multiflorum PPOX_B65_1 cDNA Contig 1 150 1314 Lolium multiflorum PPOX_B68_1 cDNA Contig 1 108 1315 Lolium multiflorum PPOX_B68_1 cDNA Contig 1 108 1316 Lolium multiflorum PPOX_B66_1 cDNA Contig 1 144 1317 Lolium multiflorum PPOX_B66_1 cDNA Contig 1 144 1318 Lolium multiflorum PPOX_B67_1 cDNA Contig 1 114 1319 Lolium multiflorum PPOX_B67_1 cDNA Contig 1 114 1320 Lolium multiflorum PPOX_B69_1 gDNA Contig 1 200 1321 Lolium multiflorum PPOX_B69_1 gDNA Contig 1 200 1322 Lolium multiflorum PPOX_B69_2 gDNA Contig 176 375 1323 Lolium multiflorum PPOX_B69_2 gDNA Contig 176 375 1324 Lolium multiflorum PPOX_B69_3 gDNA Contig 351 550 1325 Lolium multiflorum PPOX_B69_3 gDNA Contig 351 550 1326 Lolium multiflorum PPOX_B69_4 gDNA Contig 526 725 1327 Lolium multiflorum PPOX_B69_4 gDNA Contig 526 725 1328 Lolium multiflorum PPOX_B69_5 gDNA Contig 701 900 1329 Lolium multiflorum PPOX_B69_5 gDNA Contig 701 900 1330 Lolium multiflorum PPOX_B69_6 gDNA Contig 876 1075 1331 Lolium multiflorum PPOX_B69_6 gDNA Contig 876 1075 1332 Lolium multiflorum PPOX_B69_7 gDNA Contig 1051 1250 1333 Lolium multiflorum PPOX_B69_7 gDNA Contig 1051 1250 1334 Lolium multiflorum PPOX_B69_8 gDNA Contig 1226 1425 1335 Lolium multiflorum PPOX_B69_8 gDNA Contig 1226 1425 1336 Lolium multiflorum PPOX_B69_9 gDNA Contig 1401 1600 1337 Lolium multiflorum PPOX_B69_9 gDNA Contig 1401 1600 1338 Lolium multiflorum PPOX_B69_10 gDNA Contig 1576 1775 1339 Lolium multiflorum PPOX_B69_10 gDNA Contig 1576 1775 1340 Lolium multiflorum PPOX_B69_11 gDNA Contig 1751 1950 1341 Lolium multiflorum PPOX_B69_11 gDNA Contig 1751 1950 1342 Lolium multiflorum PPOX_B69_12 gDNA Contig 1926 2125 1343 Lolium multiflorum PPOX_B69_12 gDNA Contig 1926 2125 1344 Lolium multiflorum PPOX_B69_13 gDNA Contig 2101 2300 1345 Lolium multiflorum PPOX_B69_13 gDNA Contig 2101 2300 1346 Lolium multiflorum PPOX_B69_14 gDNA Contig 2276 2475 1347 Lolium multiflorum PPOX_B69_14 gDNA Contig 2276 2475 1348 Lolium multiflorum PPOX_B69_15 gDNA Contig 2451 2650 1349 Lolium multiflorum PPOX_B69_15 gDNA Contig 2451 2650 1350 Lolium multiflorum PPOX_B69_16 gDNA Contig 2626 2825 1351 Lolium multiflorum PPOX_B69_16 gDNA Contig 2626 2825 1352 Lolium multiflorum PPOX_B69_17 gDNA Contig 2801 3000 1353 Lolium multiflorum PPOX_B69_17 gDNA Contig 2801 3000 1354 Lolium multiflorum PPOX_B62_1 cDNA Contig 1 200 1355 Lolium multiflorum PPOX_B62_1 cDNA Contig 1 200 1356 Lolium multiflorum PPOX_B62_2 cDNA Contig 176 375 1357 Lolium multiflorum PPOX_B62_2 cDNA Contig 176 375 1358 Lolium multiflorum PPOX_B62_3 cDNA Contig 351 550 1359 Lolium multiflorum PPOX_B62_3 cDNA Contig 351 550 1360 Lolium multiflorum PPOX_B62_4 cDNA Contig 526 725 1361 Lolium multiflorum PPOX_B62_4 cDNA Contig 526 725 1362 Lolium multiflorum PPOX_B62_5 cDNA Contig 701 900 1363 Lolium multiflorum PPOX_B62_5 cDNA Contig 701 900 1364 Lolium multiflorum PPOX_B62_6 cDNA Contig 876 1075 1365 Lolium multiflorum PPOX_B62_6 cDNA Contig 876 1075 1366 Lolium multiflorum PPOX_B70_1 gDNA Contig 1 200 1367 Lolium multiflorum PPOX_B70_1 gDNA Contig 1 200 1368 Lolium multiflorum PPOX_B70_2 gDNA Contig 176 375 1369 Lolium multiflorum PPOX_B70_2 gDNA Contig 176 375 1370 Lolium multiflorum PPOX_B70_3 gDNA Contig 351 550 1371 Lolium multiflorum PPOX_B70_3 gDNA Contig 351 550 1372 Lolium multiflorum PPOX_B70_4 gDNA Contig 526 725 1373 Lolium multiflorum PPOX_B70_4 gDNA Contig 526 725 1374 Lolium multiflorum PPOX_B71_1 gDNA Contig 1 200 1375 Lolium multiflorum PPOX_B71_1 gDNA Contig 1 200 1376 Lolium multiflorum PPOX_B71_2 gDNA Contig 176 375 1377 Lolium multiflorum PPOX_B71_2 gDNA Contig 176 375 1378 Lolium multiflorum PPOX_B63_1 cDNA Contig 1 200 1379 Lolium multiflorum PPOX_B63_1 cDNA Contig 1 200 1380 Lolium multiflorum PPOX_B64_1 cDNA Contig 1 200 1381 Lolium multiflorum PPOX_B64_1 cDNA Contig 1 200

The gene sequences and fragments of Table 1 were compared and 21-mers of contiguous polynucleotides were identified that had homology across the various PPG oxidase gene sequences. The purpose is to identify trigger molecules that are useful as herbicidal molecules or in combination with a PPG oxidase inhibitor herbicide across a broad range of weed species. The sequences shown in Table 3 represent the 21-mers that were present in the PPG oxidase gene of at least eight of the weed species of Table 1. It is contemplated that additional 21-mers can be selected from the sequences of Table 1 that are specific for a single weed species or a few weeds species within a genus or trigger molecules that are at least 18 contiguous nucleotides, at least 19 contiguous nucleotides, at least 20 contiguous nucleotides or at least 21 contiguous nucleotides in length and at least 85 percent identical to a PPG oxidase gene sequence selected from the group consisting of SEQ ID NOs:1-71 or fragment thereof.

By this method, it is possible to identify an oligonucleotide or several oligonucleotides that are the most efficacious trigger molecule to effect plant sensitivity to PPG oxidase inhibitor or modulation of PPG oxidase gene expression. The modulation of PPG oxidase gene expression is determined by the detection of PPG oxidase siRNA molecules specific to PPG oxidase gene or by an observation of a reduction in the amount of PPG oxidase RNA transcript produced relative to an untreated plant. Detection of siRNA can be accomplished, for example, using kits such as mirVana (Ambion, Austin Tex.) and mirPremier (Sigma-Aldrich, St Louis, Mo.).

The target DNA sequence isolated from genomic (gDNA) and coding DNA (cDNA) from the various weedy plant species for the PPG oxidase gene and the assembled contigs as set forth in SEQ ID Nos: 1-71 were divided into fragments as shown in Table 3 below and as set forth in SEQ ID NOs 1382-2221.

TABLE 3 SEQ #Spe- ID NO Gene cies Species 1382 PPOX 10 Amaranthus albus, Amaranthus graecizans, Amaranthus hybridus, Amaranthus. Lividus, Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus, Amaranthus thunbergii, Amaranthus viridis (“Amaranthus sp.”), Commelina diffusa 1383 PPOX 10 Amaranthus sp., Chenopodium album 1384 PPOX 10 Amaranthus sp., Chenopodium album 1385 PPOX 10 Amaranthus sp., Commelina diffusa 1386 PPOX 10 Amaranthus sp., Chenopodium album 1387 PPOX 10 Amaranthus sp., Chenopodium album 1388 PPOX 10 Amaranthus sp., Chenopodium album 1389 PPOX 10 Amaranthus sp., Commelina diffusa 1390 PPOX 10 Amaranthus sp., Chenopodium album 1391 PPOX 10 Amaranthus sp., Chenopodium album 1392 PPOX 10 Amaranthus sp., Chenopodium album 1393 PPOX 10 Amaranthus sp., Chenopodium album 1394 PPOX 10 Amaranthus sp., Commelina diffusa 1395 PPOX 10 Amaranthus sp., Chenopodium album 1396 PPOX 9 Amaranthus sp. 1397 PPOX 9 Amaranthus sp. 1398 PPOX 9 Amaranthus sp. 1399 PPOX 9 Amaranthus sp. 1400 PPOX 9 Amaranthus sp. 1401 PPOX 9 Amaranthus sp. 1402 PPOX 9 Amaranthus sp. 1403 PPOX 9 Amaranthus sp. 1404 PPOX 9 Amaranthus sp. 1405 PPOX 9 Amaranthus sp. 1406 PPOX 9 Amaranthus sp. 1407 PPOX 9 Amaranthus sp. 1408 PPOX 9 Amaranthus sp. 1409 PPOX 9 Amaranthus sp. 1410 PPOX 9 Amaranthus sp. 1411 PPOX 9 Amaranthus sp. 1412 PPOX 9 Amaranthus sp. 1413 PPOX 9 Amaranthus sp. 1414 PPOX 9 Amaranthus sp. 1415 PPOX 9 Amaranthus sp. 1416 PPOX 9 Amaranthus sp. 1417 PPOX 9 Amaranthus sp. 1418 PPOX 9 Amaranthus sp. 1419 PPOX 9 Amaranthus sp. 1420 PPOX 9 Amaranthus sp. 1421 PPOX 9 Amaranthus sp. 1422 PPOX 9 Amaranthus sp. 1423 PPOX 9 Amaranthus sp. 1424 PPOX 9 Amaranthus sp. 1425 PPOX 9 Amaranthus sp. 1426 PPOX 9 Amaranthus sp. 1427 PPOX 9 Amaranthus sp. 1428 PPOX 9 Amaranthus sp. 1429 PPOX 9 Amaranthus sp. 1430 PPOX 9 Amaranthus sp. 1431 PPOX 9 Amaranthus sp. 1432 PPOX 9 Amaranthus sp. 1433 PPOX 9 Amaranthus sp. 1434 PPOX 9 Amaranthus sp. 1435 PPOX 9 Amaranthus sp. 1436 PPOX 9 Amaranthus sp. 1437 PPOX 9 Amaranthus sp. 1438 PPOX 9 Amaranthus sp. 1439 PPOX 9 Amaranthus sp. 1440 PPOX 9 Amaranthus sp. 1441 PPOX 9 Amaranthus sp. 1442 PPOX 9 Amaranthus sp. 1443 PPOX 9 Amaranthus sp. 1444 PPOX 9 Amaranthus sp. 1445 PPOX 9 Amaranthus sp. 1446 PPOX 9 Amaranthus sp. 1447 PPOX 9 Amaranthus sp. 1448 PPOX 9 Amaranthus sp. 1449 PPOX 9 Amaranthus sp. 1450 PPOX 9 Amaranthus sp. 1451 PPOX 9 Amaranthus sp. 1452 PPOX 9 Amaranthus sp. 1453 PPOX 9 Amaranthus sp. 1454 PPOX 9 Amaranthus sp. 1455 PPOX 9 Amaranthus sp. 1456 PPOX 9 Amaranthus sp. 1457 PPOX 9 Amaranthus sp. 1458 PPOX 9 Amaranthus sp. 1459 PPOX 9 Amaranthus sp. 1460 PPOX 9 Amaranthus sp. 1461 PPOX 9 Amaranthus sp. 1462 PPOX 9 Amaranthus sp. 1463 PPOX 9 Amaranthus sp. 1464 PPOX 9 Amaranthus sp. 1465 PPOX 9 Amaranthus sp. 1466 PPOX 9 Amaranthus sp. 1467 PPOX 9 Amaranthus sp. 1468 PPOX 9 Amaranthus sp. 1469 PPOX 9 Amaranthus sp. 1470 PPOX 9 Amaranthus sp. 1471 PPOX 9 Amaranthus sp. 1472 PPOX 9 Amaranthus sp. 1473 PPOX 9 Amaranthus sp. 1474 PPOX 9 Amaranthus sp. 1475 PPOX 9 Amaranthus sp. 1476 PPOX 9 Amaranthus sp. 1477 PPOX 9 Amaranthus sp. 1478 PPOX 9 Amaranthus sp. 1479 PPOX 9 Amaranthus sp. 1480 PPOX 9 Amaranthus sp. 1481 PPOX 9 Amaranthus sp. 1482 PPOX 9 Amaranthus sp. 1483 PPOX 9 Amaranthus sp. 1484 PPOX 9 Amaranthus sp. 1485 PPOX 9 Amaranthus sp. 1486 PPOX 9 Amaranthus sp. 1487 PPOX 9 Amaranthus sp. 1488 PPOX 9 Amaranthus sp. 1489 PPOX 9 Amaranthus sp. 1490 PPOX 9 Amaranthus sp. 1491 PPOX 9 Amaranthus sp. 1492 PPOX 9 Amaranthus sp. 1493 PPOX 9 Amaranthus sp. 1494 PPOX 9 Amaranthus sp. 1495 PPOX 9 Amaranthus sp. 1496 PPOX 9 Amaranthus sp. 1497 PPOX 9 Amaranthus sp. 1498 PPOX 9 Amaranthus sp. 1499 PPOX 9 Amaranthus sp. 1500 PPOX 9 Amaranthus sp. 1501 PPOX 9 Amaranthus sp. 1502 PPOX 9 Amaranthus sp. 1503 PPOX 9 Amaranthus sp. 1504 PPOX 9 Amaranthus sp. 1505 PPOX 9 Amaranthus sp. 1506 PPOX 9 Amaranthus sp. 1507 PPOX 9 Amaranthus sp. 1508 PPOX 9 Amaranthus sp. 1509 PPOX 9 Amaranthus sp. 1510 PPOX 9 Amaranthus sp. 1511 PPOX 9 Amaranthus sp. 1512 PPOX 9 Amaranthus sp. 1513 PPOX 9 Amaranthus sp. 1514 PPOX 9 Amaranthus sp. 1515 PPOX 9 Amaranthus sp. 1516 PPOX 9 Amaranthus sp. 1517 PPOX 9 Amaranthus sp. 1518 PPOX 9 Amaranthus sp. 1519 PPOX 9 Amaranthus sp. 1520 PPOX 9 Amaranthus sp. 1521 PPOX 9 Amaranthus sp. 1522 PPOX 9 Amaranthus sp. 1523 PPOX 9 Amaranthus sp. 1524 PPOX 9 Amaranthus sp. 1525 PPOX 9 Amaranthus sp. 1526 PPOX 9 Amaranthus sp. 1527 PPOX 9 Amaranthus sp. 1528 PPOX 9 Amaranthus sp. 1529 PPOX 9 Amaranthus sp. 1530 PPOX 9 Amaranthus sp. 1531 PPOX 9 Amaranthus sp. 1532 PPOX 9 Amaranthus sp. 1533 PPOX 9 Amaranthus sp. 1534 PPOX 9 Amaranthus sp. 1535 PPOX 9 Amaranthus sp. 1536 PPOX 9 Amaranthus sp. 1537 PPOX 9 Amaranthus sp. 1538 PPOX 9 Amaranthus sp. 1539 PPOX 9 Amaranthus sp. 1540 PPOX 9 Amaranthus sp. 1541 PPOX 9 Amaranthus sp. 1542 PPOX 9 Amaranthus sp. 1543 PPOX 9 Amaranthus sp. 1544 PPOX 9 Amaranthus sp. 1545 PPOX 9 Amaranthus sp. 1546 PPOX 9 Amaranthus sp. 1547 PPOX 9 Amaranthus sp. 1548 PPOX 9 Amaranthus sp. 1549 PPOX 9 Amaranthus sp. 1550 PPOX 9 Amaranthus sp. 1551 PPOX 9 Amaranthus sp. 1552 PPOX 9 Amaranthus sp. 1553 PPOX 9 Amaranthus sp. 1554 PPOX 9 Amaranthus sp. 1555 PPOX 9 Amaranthus sp. 1556 PPOX 9 Amaranthus sp. 1557 PPOX 9 Amaranthus sp. 1558 PPOX 9 Amaranthus sp. 1559 PPOX 9 Amaranthus sp. 1560 PPOX 9 Amaranthus sp. 1561 PPOX 9 Amaranthus sp. 1562 PPOX 9 Amaranthus sp. 1563 PPOX 9 Amaranthus sp. 1564 PPOX 9 Amaranthus sp. 1565 PPOX 9 Amaranthus sp. 1566 PPOX 9 Amaranthus sp. 1567 PPOX 9 Amaranthus sp. 1568 PPOX 9 Amaranthus sp. 1569 PPOX 9 Amaranthus sp. 1570 PPOX 9 Amaranthus sp. 1571 PPOX 9 Amaranthus sp. 1572 PPOX 9 Amaranthus sp. 1573 PPOX 9 Amaranthus sp. 1574 PPOX 9 Amaranthus sp. 1575 PPOX 9 Amaranthus sp. 1576 PPOX 9 Amaranthus sp. 1577 PPOX 9 Amaranthus sp. 1578 PPOX 9 Amaranthus sp. 1579 PPOX 9 Amaranthus sp. 1580 PPOX 9 Amaranthus sp. 1581 PPOX 9 Amaranthus sp. 1582 PPOX 9 Amaranthus sp. 1583 PPOX 9 Amaranthus sp. 1584 PPOX 9 Amaranthus sp. 1585 PPOX 9 Amaranthus sp. 1586 PPOX 9 Amaranthus sp. 1587 PPOX 9 Amaranthus sp. 1588 PPOX 9 Amaranthus sp. 1589 PPOX 9 Amaranthus sp. 1590 PPOX 9 Amaranthus sp. 1591 PPOX 9 Amaranthus sp. 1592 PPOX 9 Amaranthus sp. 1593 PPOX 9 Amaranthus sp. 1594 PPOX 9 Amaranthus sp. 1595 PPOX 9 Amaranthus sp. 1596 PPOX 9 Amaranthus sp. 1597 PPOX 9 Amaranthus sp. 1598 PPOX 9 Amaranthus sp. 1599 PPOX 9 Amaranthus sp. 1600 PPOX 9 Amaranthus sp. 1601 PPOX 9 Amaranthus sp. 1602 PPOX 9 Amaranthus sp. 1603 PPOX 9 Amaranthus sp. 1604 PPOX 9 Amaranthus sp. 1605 PPOX 9 Amaranthus sp. 1606 PPOX 9 Amaranthus sp. 1607 PPOX 9 Amaranthus sp. 1608 PPOX 9 Amaranthus sp. 1609 PPOX 9 Amaranthus sp. 1610 PPOX 9 Amaranthus sp. 1611 PPOX 9 Amaranthus sp. 1612 PPOX 9 Amaranthus sp. 1613 PPOX 9 Amaranthus sp. 1614 PPOX 9 Amaranthus sp. 1615 PPOX 9 Amaranthus sp. 1616 PPOX 9 Amaranthus sp. 1617 PPOX 9 Amaranthus sp. 1618 PPOX 9 Amaranthus sp. 1619 PPOX 9 Amaranthus sp. 1620 PPOX 9 Amaranthus sp. 1621 PPOX 9 Amaranthus sp. 1622 PPOX 9 Amaranthus sp. 1623 PPOX 9 Amaranthus sp. 1624 PPOX 9 Amaranthus sp. 1625 PPOX 9 Amaranthus sp. 1626 PPOX 9 Amaranthus sp. 1627 PPOX 9 Amaranthus sp. 1628 PPOX 9 Amaranthus sp. 1629 PPOX 9 Amaranthus sp. 1630 PPOX 9 Amaranthus sp. 1631 PPOX 9 Amaranthus sp. 1632 PPOX 9 Amaranthus sp. 1633 PPOX 9 Amaranthus sp. 1634 PPOX 9 Amaranthus sp. 1635 PPOX 9 Amaranthus sp. 1636 PPOX 9 Amaranthus sp. 1637 PPOX 9 Amaranthus sp. 1638 PPOX 9 Amaranthus sp. 1639 PPOX 9 Amaranthus sp. 1640 PPOX 9 Amaranthus sp. 1641 PPOX 9 Amaranthus sp. 1642 PPOX 9 Amaranthus sp. 1643 PPOX 9 Amaranthus sp. 1644 PPOX 9 Amaranthus sp. 1645 PPOX 9 Amaranthus sp. 1646 PPOX 9 Amaranthus sp. 1647 PPOX 9 Amaranthus sp. 1648 PPOX 9 Amaranthus sp. 1649 PPOX 9 Amaranthus sp. 1650 PPOX 9 Amaranthus sp. 1651 PPOX 9 Amaranthus sp. 1652 PPOX 9 Amaranthus sp. 1653 PPOX 9 Amaranthus sp. 1654 PPOX 9 Amaranthus sp. 1655 PPOX 9 Amaranthus sp. 1656 PPOX 9 Amaranthus sp. 1657 PPOX 9 Amaranthus sp. 1658 PPOX 9 Amaranthus sp. 1659 PPOX 9 Amaranthus sp. 1660 PPOX 9 Amaranthus sp. 1661 PPOX 9 Amaranthus sp. 1662 PPOX 9 Amaranthus sp. 1663 PPOX 9 Amaranthus sp. 1664 PPOX 9 Amaranthus sp. 1665 PPOX 9 Amaranthus sp. 1666 PPOX 9 Amaranthus sp. 1667 PPOX 9 Amaranthus sp. 1668 PPOX 9 Amaranthus sp. 1669 PPOX 9 Amaranthus sp. 1670 PPOX 9 Amaranthus sp. 1671 PPOX 9 Amaranthus sp. 1672 PPOX 9 Amaranthus sp. 1673 PPOX 9 Amaranthus sp. 1674 PPOX 9 Amaranthus sp. 1675 PPOX 9 Amaranthus sp. 1676 PPOX 9 Amaranthus sp. 1677 PPOX 9 Amaranthus sp. 1678 PPOX 9 Amaranthus sp. 1679 PPOX 9 Amaranthus sp. 1680 PPOX 9 Amaranthus sp. 1681 PPOX 9 Amaranthus sp. 1682 PPOX 9 Amaranthus sp. 1683 PPOX 9 Amaranthus sp. 1684 PPOX 9 Amaranthus sp. 1685 PPOX 9 Amaranthus sp. 1686 PPOX 9 Amaranthus sp. 1687 PPOX 9 Amaranthus sp. 1688 PPOX 9 Amaranthus sp. 1689 PPOX 9 Amaranthus sp. 1690 PPOX 9 Amaranthus sp. 1691 PPOX 9 Amaranthus sp. 1692 PPOX 9 Amaranthus sp. 1693 PPOX 9 Amaranthus sp. 1694 PPOX 9 Amaranthus sp. 1695 PPOX 9 Amaranthus sp. 1696 PPOX 9 Amaranthus sp. 1697 PPOX 9 Amaranthus sp. 1698 PPOX 9 Amaranthus sp. 1699 PPOX 9 Amaranthus sp. 1700 PPOX 9 Amaranthus sp. 1701 PPOX 9 Amaranthus sp. 1702 PPOX 9 Amaranthus sp. 1703 PPOX 9 Amaranthus sp. 1704 PPOX 9 Amaranthus sp. 1705 PPOX 9 Amaranthus sp. 1706 PPOX 9 Amaranthus sp. 1707 PPOX 9 Amaranthus sp. 1708 PPOX 9 Amaranthus sp. 1709 PPOX 9 Amaranthus sp. 1710 PPOX 9 Amaranthus sp. 1711 PPOX 9 Amaranthus sp. 1712 PPOX 9 Amaranthus sp. 1713 PPOX 9 Amaranthus sp. 1714 PPOX 9 Amaranthus sp. 1715 PPOX 9 Amaranthus sp. 1716 PPOX 9 Amaranthus sp. 1717 PPOX 9 Amaranthus sp. 1718 PPOX 9 Amaranthus sp. 1719 PPOX 9 Amaranthus sp. 1720 PPOX 9 Amaranthus sp. 1721 PPOX 9 Amaranthus sp. 1722 PPOX 9 Amaranthus sp. 1723 PPOX 9 Amaranthus sp. 1724 PPOX 9 Amaranthus sp. 1725 PPOX 9 Amaranthus sp. 1726 PPOX 9 Amaranthus sp. 1727 PPOX 9 Amaranthus sp. 1728 PPOX 9 Amaranthus sp. 1729 PPOX 9 Amaranthus sp. 1730 PPOX 9 Amaranthus sp. 1731 PPOX 9 Amaranthus sp. 1732 PPOX 9 Amaranthus sp. 1733 PPOX 9 Amaranthus sp. 1734 PPOX 9 Amaranthus sp. 1735 PPOX 9 Amaranthus sp. 1736 PPOX 9 Amaranthus sp. 1737 PPOX 9 Amaranthus sp. 1738 PPOX 9 Amaranthus sp. 1739 PPOX 9 Amaranthus sp. 1740 PPOX 9 Amaranthus sp. 1741 PPOX 9 Amaranthus sp. 1742 PPOX 9 Amaranthus sp. 1743 PPOX 9 Amaranthus sp. 1744 PPOX 9 Amaranthus sp. 1745 PPOX 9 Amaranthus sp. 1746 PPOX 9 Amaranthus sp. 1747 PPOX 9 Amaranthus sp. 1748 PPOX 9 Amaranthus sp. 1749 PPOX 9 Amaranthus sp. 1750 PPOX 9 Amaranthus sp. 1751 PPOX 9 Amaranthus sp. 1752 PPOX 9 Amaranthus sp. 1753 PPOX 9 Amaranthus sp. 1754 PPOX 9 Amaranthus sp. 1755 PPOX 9 Amaranthus sp. 1756 PPOX 9 Amaranthus sp. 1757 PPOX 9 Amaranthus sp. 1758 PPOX 9 Amaranthus sp. 1759 PPOX 9 Amaranthus sp. 1760 PPOX 9 Amaranthus sp. 1761 PPOX 9 Amaranthus sp. 1762 PPOX 9 Amaranthus sp. 1763 PPOX 9 Amaranthus sp. 1764 PPOX 9 Amaranthus sp. 1765 PPOX 9 Amaranthus sp. 1766 PPOX 9 Amaranthus sp. 1767 PPOX 9 Amaranthus sp. 1768 PPOX 9 Amaranthus sp. 1769 PPOX 9 Amaranthus sp. 1770 PPOX 9 Amaranthus sp. 1771 PPOX 9 Amaranthus sp. 1772 PPOX 9 Amaranthus sp. 1773 PPOX 9 Amaranthus sp. 1774 PPOX 9 Amaranthus sp. 1775 PPOX 9 Amaranthus sp. 1776 PPOX 9 Amaranthus sp. 1777 PPOX 9 Amaranthus sp. 1778 PPOX 9 Amaranthus sp. 1779 PPOX 9 Amaranthus sp. 1780 PPOX 9 Amaranthus sp. 1781 PPOX 9 Amaranthus sp. 1782 PPOX 9 Amaranthus sp. 1783 PPOX 9 Amaranthus sp. 1784 PPOX 9 Amaranthus sp. 1785 PPOX 9 Amaranthus sp. 1786 PPOX 9 Amaranthus sp. 1787 PPOX 9 Amaranthus sp. 1788 PPOX 9 Amaranthus sp. 1789 PPOX 9 Amaranthus sp. 1790 PPOX 9 Amaranthus sp. 1791 PPOX 9 Amaranthus sp. 1792 PPOX 9 Amaranthus sp. 1793 PPOX 9 Amaranthus sp. 1794 PPOX 9 Amaranthus sp. 1795 PPOX 9 Amaranthus sp. 1796 PPOX 9 Amaranthus sp. 1797 PPOX 9 Amaranthus sp. 1798 PPOX 9 Amaranthus sp. 1799 PPOX 9 Amaranthus sp. 1800 PPOX 9 Amaranthus sp. 1801 PPOX 9 Amaranthus sp. 1802 PPOX 9 Amaranthus sp. 1803 PPOX 9 Amaranthus sp. 1804 PPOX 9 Amaranthus sp. 1805 PPOX 9 Amaranthus sp. 1806 PPOX 9 Amaranthus sp. 1807 PPOX 9 Amaranthus sp. 1808 PPOX 9 Amaranthus sp. 1809 PPOX 9 Amaranthus sp. 1810 PPOX 9 Amaranthus sp. 1811 PPOX 9 Amaranthus sp. 1812 PPOX 9 Amaranthus sp. 1813 PPOX 9 Amaranthus sp. 1814 PPOX 9 Amaranthus sp. 1815 PPOX 9 Amaranthus sp. 1816 PPOX 9 Amaranthus sp. 1817 PPOX 9 Amaranthus sp. 1818 PPOX 9 Amaranthus sp. 1819 PPOX 9 Amaranthus sp. 1820 PPOX 9 Amaranthus sp. 1821 PPOX 9 Amaranthus sp. 1822 PPOX 9 Amaranthus sp. 1823 PPOX 9 Amaranthus sp. 1824 PPOX 9 Amaranthus sp. 1825 PPOX 9 Amaranthus sp. 1826 PPOX 9 Amaranthus sp. 1827 PPOX 9 Amaranthus sp. 1828 PPOX 9 Amaranthus sp. 1829 PPOX 9 Amaranthus sp. 1830 PPOX 9 Amaranthus sp. 1831 PPOX 9 Amaranthus sp. 1832 PPOX 9 Amaranthus sp. 1833 PPOX 9 Amaranthus sp. 1834 PPOX 9 Amaranthus sp. 1835 PPOX 9 Amaranthus sp. 1836 PPOX 9 Amaranthus sp. 1837 PPOX 9 Amaranthus sp. 1838 PPOX 9 Amaranthus sp. 1839 PPOX 9 Amaranthus sp. 1840 PPOX 9 Amaranthus sp. 1841 PPOX 9 Amaranthus sp. 1842 PPOX 9 Amaranthus sp. 1843 PPOX 9 Amaranthus sp. 1844 PPOX 9 Amaranthus sp. 1845 PPOX 9 Amaranthus sp. 1846 PPOX 9 Amaranthus sp. 1847 PPOX 9 Amaranthus sp. 1848 PPOX 9 Amaranthus sp. 1849 PPOX 9 Amaranthus sp. 1850 PPOX 9 Amaranthus sp. 1851 PPOX 9 Amaranthus sp. 1852 PPOX 9 Amaranthus sp. 1853 PPOX 9 Amaranthus sp. 1854 PPOX 9 Amaranthus sp. 1855 PPOX 9 Amaranthus sp. 1856 PPOX 9 Amaranthus sp. 1857 PPOX 9 Amaranthus sp. 1858 PPOX 9 Amaranthus sp. 1859 PPOX 9 Amaranthus sp. 1860 PPOX 9 Amaranthus sp. 1861 PPOX 9 Amaranthus sp. 1862 PPOX 9 Amaranthus sp. 1863 PPOX 9 Amaranthus sp. 1864 PPOX 9 Amaranthus sp. 1865 PPOX 9 Amaranthus sp. 1866 PPOX 9 Amaranthus sp. 1867 PPOX 9 Amaranthus sp. 1868 PPOX 9 Amaranthus sp. 1869 PPOX 9 Amaranthus sp. 1870 PPOX 9 Amaranthus sp. 1871 PPOX 9 Amaranthus sp. 1872 PPOX 9 Amaranthus sp. 1873 PPOX 9 Amaranthus sp. 1874 PPOX 9 Amaranthus sp. 1875 PPOX 9 Amaranthus sp. 1876 PPOX 9 Amaranthus sp. 1877 PPOX 9 Amaranthus sp. 1878 PPOX 9 Amaranthus sp. 1879 PPOX 9 Amaranthus sp. 1880 PPOX 9 Amaranthus sp. 1881 PPOX 9 Amaranthus sp. 1882 PPOX 9 Amaranthus sp. 1883 PPOX 9 Amaranthus sp. 1884 PPOX 9 Amaranthus sp. 1885 PPOX 9 Amaranthus sp. 1886 PPOX 9 Amaranthus sp. 1887 PPOX 9 Amaranthus sp. 1888 PPOX 9 Amaranthus sp. 1889 PPOX 9 Amaranthus sp. 1890 PPOX 9 Amaranthus sp. 1891 PPOX 9 Amaranthus sp. 1892 PPOX 9 Amaranthus sp. 1893 PPOX 9 Amaranthus sp. 1894 PPOX 9 Amaranthus sp. 1895 PPOX 9 Amaranthus sp. 1896 PPOX 9 Amaranthus sp. 1897 PPOX 9 Amaranthus sp. 1898 PPOX 9 Amaranthus sp. 1899 PPOX 9 Amaranthus sp. 1900 PPOX 9 Amaranthus sp. 1901 PPOX 9 Amaranthus sp. 1902 PPOX 9 Amaranthus sp. 1903 PPOX 9 Amaranthus sp. 1904 PPOX 9 Amaranthus sp. 1905 PPOX 9 Amaranthus sp. 1906 PPOX 9 Amaranthus sp. 1907 PPOX 9 Amaranthus sp. 1908 PPOX 9 Amaranthus sp. 1909 PPOX 9 Amaranthus sp. 1910 PPOX 9 Amaranthus sp. 1911 PPOX 9 Amaranthus sp. 1912 PPOX 9 Amaranthus sp. 1913 PPOX 9 Amaranthus sp. 1914 PPOX 9 Amaranthus sp. 1915 PPOX 9 Amaranthus sp. 1916 PPOX 9 Amaranthus sp. 1917 PPOX 9 Amaranthus sp. 1918 PPOX 9 Amaranthus sp. 1919 PPOX 9 Amaranthus sp. 1920 PPOX 9 Amaranthus sp. 1921 PPOX 9 Amaranthus sp. 1922 PPOX 9 Amaranthus sp. 1923 PPOX 9 Amaranthus sp. 1924 PPOX 9 Amaranthus sp. 1925 PPOX 9 Amaranthus sp. 1926 PPOX 9 Amaranthus sp. 1927 PPOX 9 Amaranthus sp. 1928 PPOX 9 Amaranthus sp. 1929 PPOX 9 Amaranthus sp. 1930 PPOX 9 Amaranthus sp. 1931 PPOX 9 Amaranthus sp. 1932 PPOX 9 Amaranthus sp. 1933 PPOX 9 Amaranthus sp. 1934 PPOX 9 Amaranthus sp. 1935 PPOX 9 Amaranthus sp. 1936 PPOX 9 Amaranthus sp. 1937 PPOX 9 Amaranthus sp. 1938 PPOX 9 Amaranthus sp. 1939 PPOX 9 Amaranthus sp. 1940 PPOX 9 Amaranthus sp. 1941 PPOX 9 Amaranthus sp. 1942 PPOX 9 Amaranthus sp. 1943 PPOX 9 Amaranthus sp. 1944 PPOX 9 Amaranthus sp. 1945 PPOX 9 Amaranthus sp. 1946 PPOX 9 Amaranthus sp. 1947 PPOX 9 Amaranthus sp. 1948 PPOX 9 Amaranthus sp. 1949 PPOX 9 Amaranthus sp. 1950 PPOX 9 Amaranthus sp. 1951 PPOX 9 Amaranthus sp. 1952 PPOX 9 Amaranthus sp. 1953 PPOX 9 Amaranthus sp. 1954 PPOX 9 Amaranthus sp. 1955 PPOX 9 Amaranthus sp. 1956 PPOX 9 Amaranthus sp. 1957 PPOX 9 Amaranthus sp. 1958 PPOX 9 Amaranthus sp. 1959 PPOX 9 Amaranthus sp. 1960 PPOX 9 Amaranthus sp. 1961 PPOX 9 Amaranthus sp. 1962 PPOX 9 Amaranthus sp. 1963 PPOX 9 Amaranthus sp. 1964 PPOX 9 Amaranthus sp. 1965 PPOX 9 Amaranthus sp. 1966 PPOX 9 Amaranthus sp. 1967 PPOX 9 Amaranthus sp. 1968 PPOX 9 Amaranthus sp. 1969 PPOX 9 Amaranthus sp. 1970 PPOX 9 Amaranthus sp. 1971 PPOX 9 Amaranthus sp. 1972 PPOX 9 Amaranthus sp. 1973 PPOX 9 Amaranthus sp. 1974 PPOX 9 Amaranthus sp. 1975 PPOX 9 Amaranthus sp. 1976 PPOX 9 Amaranthus sp. 1977 PPOX 9 Amaranthus sp. 1978 PPOX 9 Amaranthus sp. 1979 PPOX 9 Amaranthus sp. 1980 PPOX 9 Amaranthus sp. 1981 PPOX 9 Amaranthus sp. 1982 PPOX 9 Amaranthus sp. 1983 PPOX 9 Amaranthus sp. 1984 PPOX 9 Amaranthus sp. 1985 PPOX 9 Amaranthus sp. 1986 PPOX 9 Amaranthus sp. 1987 PPOX 9 Amaranthus sp. 1988 PPOX 9 Amaranthus sp. 1989 PPOX 9 Amaranthus sp. 1990 PPOX 9 Amaranthus sp. 1991 PPOX 9 Amaranthus sp. 1992 PPOX 9 Amaranthus sp. 1993 PPOX 9 Amaranthus sp. 1994 PPOX 9 Amaranthus sp. 1995 PPOX 9 Amaranthus sp. 1996 PPOX 9 Amaranthus sp. 1997 PPOX 9 Amaranthus sp. 1998 PPOX 9 Amaranthus sp. 1999 PPOX 9 Amaranthus sp. 2000 PPOX 9 Amaranthus sp. 2001 PPOX 9 Amaranthus sp. 2002 PPOX 9 Amaranthus sp. 2003 PPOX 9 Amaranthus sp. 2004 PPOX 9 Amaranthus sp. 2005 PPOX 9 Amaranthus sp. 2006 PPOX 9 Amaranthus sp. 2007 PPOX 9 Amaranthus sp. 2008 PPOX 9 Amaranthus sp. 2009 PPOX 9 Amaranthus sp. 2010 PPOX 9 Amaranthus sp. 2011 PPOX 9 Amaranthus sp. 2012 PPOX 9 Amaranthus sp. 2013 PPOX 9 Amaranthus sp. 2014 PPOX 9 Amaranthus sp. 2015 PPOX 9 Amaranthus sp. 2016 PPOX 9 Amaranthus sp. 2017 PPOX 9 Amaranthus sp. 2018 PPOX 9 Amaranthus sp. 2019 PPOX 9 Amaranthus sp. 2020 PPOX 9 Amaranthus sp. 2021 PPOX 9 Amaranthus sp. 2022 PPOX 9 Amaranthus sp. 2023 PPOX 9 Amaranthus sp. 2024 PPOX 9 Amaranthus sp. 2025 PPOX 9 Amaranthus sp. 2026 PPOX 9 Amaranthus sp. 2027 PPOX 9 Amaranthus sp. 2028 PPOX 9 Amaranthus sp. 2029 PPOX 9 Amaranthus sp. 2030 PPOX 9 Amaranthus sp. 2031 PPOX 9 Amaranthus sp. 2032 PPOX 9 Amaranthus sp. 2033 PPOX 9 Amaranthus sp. 2034 PPOX 9 Amaranthus sp. 2035 PPOX 9 Amaranthus sp. 2036 PPOX 9 Amaranthus sp. 2037 PPOX 9 Amaranthus sp. 2038 PPOX 9 Amaranthus sp. 2039 PPOX 9 Amaranthus sp. 2040 PPOX 9 Amaranthus sp. 2041 PPOX 9 Amaranthus sp. 2042 PPOX 9 Amaranthus sp. 2043 PPOX 9 Amaranthus sp. 2044 PPOX 9 Amaranthus sp. 2045 PPOX 9 Amaranthus sp. 2046 PPOX 9 Amaranthus sp. 2047 PPOX 9 Amaranthus sp. 2048 PPOX 9 Amaranthus sp. 2049 PPOX 9 Amaranthus sp. 2050 PPOX 9 Amaranthus sp. 2051 PPOX 9 Amaranthus sp. 2052 PPOX 9 Amaranthus sp. 2053 PPOX 9 Amaranthus sp. 2054 PPOX 9 Amaranthus sp. 2055 PPOX 9 Amaranthus sp. 2056 PPOX 9 Amaranthus sp. 2057 PPOX 9 Amaranthus sp. 2058 PPOX 9 Amaranthus sp. 2059 PPOX 9 Amaranthus sp. 2060 PPOX 9 Amaranthus sp. 2061 PPOX 9 Amaranthus sp. 2062 PPOX 9 Amaranthus sp. 2063 PPOX 9 Amaranthus sp. 2064 PPOX 9 Amaranthus sp. 2065 PPOX 9 Amaranthus sp. 2066 PPOX 9 Amaranthus sp. 2067 PPOX 9 Amaranthus sp. 2068 PPOX 9 Amaranthus sp. 2069 PPOX 9 Amaranthus sp. 2070 PPOX 9 Amaranthus sp. 2071 PPOX 9 Amaranthus sp. 2072 PPOX 9 Amaranthus sp. 2073 PPOX 9 Amaranthus sp. 2074 PPOX 9 Amaranthus sp. 2075 PPOX 9 Amaranthus sp. 2076 PPOX 9 Amaranthus sp. 2077 PPOX 9 Amaranthus sp. 2078 PPOX 9 Amaranthus sp. 2079 PPOX 9 Amaranthus sp. 2080 PPOX 9 Amaranthus sp. 2081 PPOX 9 Amaranthus sp. 2082 PPOX 9 Amaranthus sp. 2083 PPOX 9 Amaranthus sp. 2084 PPOX 9 Amaranthus sp. 2085 PPOX 9 Amaranthus sp. 2086 PPOX 9 Amaranthus sp. 2087 PPOX 9 Amaranthus sp. 2088 PPOX 9 Amaranthus sp. 2089 PPOX 9 Amaranthus sp. 2090 PPOX 9 Amaranthus sp. 2091 PPOX 9 Amaranthus sp. 2092 PPOX 9 Amaranthus sp. 2093 PPOX 9 Amaranthus sp. 2094 PPOX 9 Amaranthus sp. 2095 PPOX 9 Amaranthus sp. 2096 PPOX 9 Amaranthus sp. 2097 PPOX 9 Amaranthus sp. 2098 PPOX 9 Amaranthus sp. 2099 PPOX 9 Amaranthus sp. 2100 PPOX 9 Amaranthus sp. 2101 PPOX 9 Amaranthus sp. 2102 PPOX 9 Amaranthus sp. 2103 PPOX 9 Amaranthus sp. 2104 PPOX 9 Amaranthus sp. 2105 PPOX 9 Amaranthus sp. 2106 PPOX 9 Amaranthus sp. 2107 PPOX 9 Amaranthus sp. 2108 PPOX 9 Amaranthus sp. 2109 PPOX 9 Amaranthus sp. 2110 PPOX 9 Amaranthus sp. 2111 PPOX 9 Amaranthus sp. 2112 PPOX 9 Amaranthus sp. 2113 PPOX 9 Amaranthus sp. 2114 PPOX 9 Amaranthus sp. 2115 PPOX 9 Amaranthus sp. 2116 PPOX 9 Amaranthus sp. 2117 PPOX 9 Amaranthus sp. 2118 PPOX 9 Amaranthus sp. 2119 PPOX 9 Amaranthus sp. 2120 PPOX 9 Amaranthus sp. 2121 PPOX 9 Amaranthus sp. 2122 PPOX 9 Amaranthus sp. 2123 PPOX 9 Amaranthus sp. 2124 PPOX 9 Amaranthus sp. 2125 PPOX 9 Amaranthus sp. 2126 PPOX 9 Amaranthus sp. 2127 PPOX 9 Amaranthus sp. 2128 PPOX 9 Amaranthus sp. 2129 PPOX 9 Amaranthus sp. 2130 PPOX 9 Amaranthus sp. 2131 PPOX 9 Amaranthus sp. 2132 PPOX 9 Amaranthus sp. 2133 PPOX 9 Amaranthus sp. 2134 PPOX 9 Amaranthus sp. 2135 PPOX 9 Amaranthus sp. 2136 PPOX 9 Amaranthus sp. 2137 PPOX 9 Amaranthus sp. 2138 PPOX 9 Amaranthus sp. 2139 PPOX 9 Amaranthus sp. 2140 PPOX 9 Amaranthus sp. 2141 PPOX 9 Amaranthus sp. 2142 PPOX 9 Amaranthus sp. 2143 PPOX 9 Amaranthus sp. 2144 PPOX 9 Amaranthus sp. 2145 PPOX 9 Amaranthus sp. 2146 PPOX 9 Amaranthus sp. 2147 PPOX 9 Amaranthus sp. 2148 PPOX 9 Amaranthus sp. 2149 PPOX 9 Amaranthus sp. 2150 PPOX 9 Amaranthus sp. 2151 PPOX 9 Amaranthus sp. 2152 PPOX 9 Amaranthus sp. 2153 PPOX 9 Amaranthus sp. 2154 PPOX 9 Amaranthus sp. 2155 PPOX 9 Amaranthus sp. 2156 PPOX 9 Amaranthus sp. 2157 PPOX 9 Amaranthus sp. 2158 PPOX 9 Amaranthus sp. 2159 PPOX 9 Amaranthus sp. 2160 PPOX 9 Amaranthus sp. 2161 PPOX 9 Amaranthus sp. 2162 PPOX 9 Amaranthus sp. 2163 PPOX 9 Amaranthus sp. 2164 PPOX 9 Amaranthus sp. 2165 PPOX 9 Amaranthus sp. 2166 PPOX 9 Amaranthus sp. 2167 PPOX 9 Amaranthus sp. 2168 PPOX 9 Amaranthus sp. 2169 PPOX 9 Amaranthus sp. 2170 PPOX 9 Amaranthus sp. 2171 PPOX 9 Amaranthus sp. 2172 PPOX 9 Amaranthus sp. 2173 PPOX 9 Amaranthus sp. 2174 PPOX 9 Amaranthus sp. 2175 PPOX 9 Amaranthus sp. 2176 PPOX 9 Amaranthus sp. 2177 PPOX 9 Amaranthus sp. 2178 PPOX 9 Amaranthus sp. 2179 PPOX 9 Amaranthus sp. 2180 PPOX 9 Amaranthus sp. 2181 PPOX 9 Amaranthus sp. 2182 PPOX 9 Amaranthus sp. 2183 PPOX 9 Amaranthus sp. 2184 PPOX 9 Amaranthus sp. 2185 PPOX 9 Amaranthus sp. 2186 PPOX 9 Amaranthus sp. 2187 PPOX 9 Amaranthus sp. 2188 PPOX 9 Amaranthus sp. 2189 PPOX 9 Amaranthus sp. 2190 PPOX 9 Amaranthus sp. 2191 PPOX 9 Amaranthus sp. 2192 PPOX 9 Amaranthus sp. 2193 PPOX 9 Amaranthus sp. 2194 PPOX 9 Amaranthus sp. 2195 PPOX 9 Amaranthus sp. 2196 PPOX 9 Amaranthus sp. 2197 PPOX 9 Amaranthus sp. 2198 PPOX 9 Amaranthus sp. 2199 PPOX 9 Amaranthus sp. 2200 PPOX 9 Amaranthus sp. 2201 PPOX 9 Amaranthus sp. 2202 PPOX 9 Amaranthus sp. 2203 PPOX 9 Amaranthus sp. 2204 PPOX 9 Amaranthus sp. 2205 PPOX 9 Amaranthus sp. 2206 PPOX 9 Amaranthus sp. 2207 PPOX 9 Amaranthus sp. 2208 PPOX 9 Amaranthus sp. 2209 PPOX 9 Amaranthus sp. 2210 PPOX 9 Amaranthus sp. 2211 PPOX 9 Amaranthus sp. 2212 PPOX 9 Amaranthus sp. 2213 PPOX 9 Amaranthus sp.

Example 3. Methods Used Related to Treating Plants or Plant Parts with a Topical Mixture of the Trigger Molecules

Glyphosate-sensitive Palmer amaranth (A. palmeri R-22) plants were grown in the greenhouse (30/20 C day/night T; 14 hour photoperiod) in 4 inch square pots containing Sun Gro® Redi-Earth and 3.5 kg/cubic meter Osmocote® 14-14-14 fertilizer. Palmer amaranth plants at 5 to 10 cm in height were pre-treated with a mixture of short (24-25 mer) single-strand antisense oligo DNA polynucleotides (ssDNA) targeting PPG oxidase coding or noncoding regions at 16 nmol, formulated in 10 millimolar sodium phosphate buffer (pH 6.8) containing 2% ammonium sulfate and 0.5% Silwet L-77. Plants were treated manually by pipetting 10 μL of polynucleotide solution on four fully expanded mature leaves, for a total of 40 microliters of solution per plant. Twenty-four and forty-eight hours later, the plants were treated with fomesafen (Reflex) at 22 g ai/ha and flumioxazin (Valor) at 4 g ai/ha, crop oil concentrate (COC) at 1% is added to the herbicide treatments. The formulation control is the buffer and adjuvants without the DNA polynucleotides. Four replications of each treatment were conducted. Plant height is determined just before ssDNA treatment and at intervals up to fourteen days (dat) after herbicide treatments to determine effect of the oligonucleotide and herbicide treatments. The results shown in FIG. 1 and FIG. 2 demonstrate that the oligonucleotide treatment enhanced the herbicidal activity of both fomesafen and flumioxazin.

A pool comprising eight antisense ssDNA oligonucleotides shown in Table 4 was used in the protocol as described to treat palmer amaranth plants and the individual oligonucleotides and various combinations of oligonucleotides were tested for efficacy. Surprisingly, it was necessary to have a 3 oligonucleotide pool (oligo3, 5, and 7) of the 8 oligonucleotides to reproduce the effect observed with the 8 oligonucleotide pool and combinations of only 2 of the 3 oligonucleotides was not effective to provide enhanced herbicide sensitivity. This result is illustrated in FIG. 3 for activity on Palmer amaranth. The same 3 oligonucleotides were also active on a related species, waterhemp (Amaranthus rudis).

TABLE 4  Antisense ssDNA PPG oxidase oligonucleotides OLIGO1 SEQ ID NO: 2214 GTGATATTACCTCCAACACGAT OLIGO2 SEQ ID NO: 2215 ATAGTAAGCACAGGATCGGAG OLIGO3 SEQ ID NO: 2216 CTTTCAATCCACTGTCAACCG OLIGO4 SEQ ID NO: 2217 ATCAAGCGTTCGAAGACCTCAT OLIGO5 SEQ ID NO: 2218 CAGCAATGGCGGTAGGTAACA OLIGO6 SEQ ID NO: 2219 GCAATTGCCCGAATCCTTTTA OLIGO7 SEQ ID NO: 2220 TAGCTCAAtATCAAGGTCCTA OLIGO8 SEQ ID NO: 2221 TCATAAGCACCCTCTATACAC

Example 4. A Method to Control Weeds in a Field

A method to control weeds in a field comprises the use of trigger polynucleotides that can modulate the expression of a PPG oxidase gene in one or more target weed plant species. In Table 3 (SEQ ID NOs: 1381-2221), an analysis of PPG oxidase gene sequences from seventeen plant species provided a collection of 21-mer polynucleotides that can be used in compositions to affect the growth or develop or sensitivity to PPG oxidase inhibitor herbicide to control multiple weed species in a field. A composition containing 1 or 2 or 3 or 4 or more of the polynucleotides of Table 3 would enable broad activity of the composition against the multiple weed species that occur in a field environment.

The method includes creating a composition that comprises components that include at least one polynucleotide of Table 3 or any other effective gene expression modulating polynucleotide essentially identical or essentially complementary to SEQ ID NO:1-71 or fragment thereof, a transfer agent that mobilizes the polynucleotide into a plant cell and a PPG oxidase inhibiting herbicide and optionally a polynucleotide that modulates the expression of an essential gene and optionally a herbicide that has a different mode of action relative to a PPG oxidase inhibitor. The polynucleotide of the composition includes a dsRNA, ssDNA or dsDNA or a combination thereof. A composition containing a polynucleotide can have a use rate of about 1 to 30 grams or more per acre depending on the size of the polynucleotide and the number of polynucleotides in the composition. The composition may include one or more additional herbicides as needed to provide effective multi-species weed control. A field of crop plants in need of weed plant control is treated by spray application of the composition. The composition can be provided as a tank mix, a sequential treatment of components (generally the polynucleotide followed by the herbicide), a simultaneous treatment or mixing of one or more of the components of the composition from separate containers. Treatment of the field can occur as often as needed to provide weed control and the components of the composition can be adjusted to target specific weed species or weed families.

Example 5. Herbicidal Compositions Comprising Pesticidal Agents

A method of controlling weeds and plant pest and pathogens in a field of PPG oxidase inhibitor tolerant crop plants is provided, wherein the method comprises applying a composition comprising a PPG oxidase trigger oligonucleotide, a PPG oxidase inhibitor composition and an admixture of a pest control agent. For example, the admixture comprises insecticides, fungicides, nematocides, bactericides, acaricides, growth regulators, chemosterilants, semiochemicals, repellents, attractants, pheromones, feeding stimulants or other biologically active compounds or biological agents, such as, microorganisms.

For example, the admixture comprises a fungicide compound for use on a PPG oxidase inhibitor tolerant crop plant to prevent or control plant disease caused by a plant fungal pathogen. The fungicide compound of the admixture may be a systemic or contact fungicide or mixtures of each. More particularly, the fungicide compound includes, but is not limited to, members of the chemical groups strobilurins, triazoles, chloronitriles, carboxamides and mixtures thereof. The composition may additionally have an admixture comprising an insecticidal compound or agent.

The PPG oxidase trigger oligonucleotides and PPG oxidase inhibitor (for example, fomesafen) tank mixes with fungicides, insecticides or both are tested for use in soybean and corn for control of foliar diseases and pests. Testing is conducted to develop a method for use of mixtures of the trigger oligonucleotides and fomesafen formulation and various commercially available fungicides for weed control and pest control. The field plots are planted with soybeans or corn. All plots receive a post plant application of the PPG oxidase trigger+fomesafen about 3 weeks after planting. The mixtures of trigger+fomesafen or trigger+fomesafen+fungicide+insecticides are used to treat the plots at the R1 stage of soybean development (first flowering) or tassel stage of corn. Data is taken for percent weed control at 7 and 21 days after R1 treatment, soybean safety (% necrosis, chlorosis, growth rate): 5 days after treatment, disease rating, pest ratings and yield (bushels/Acre). These mixtures and treatments are designed to provide simultaneous weed and pest control, such as fungal pest control, for example, leaf rust disease; and insect pest control, for example, aphids, armyworms, loopers, beetles, stinkbugs, and leaf hoppers.

Agricultural chemicals are provided in containers suitable for safe storage, transportation and distribution, stability of the chemical compositions, mixing with solvents and instructions for use. A container of a mixture of a trigger oligonucleotide+a herbicice+fungicide compound, or a mixture of a trigger oligonucleotide+herbicide compound and an insecticide compound, or a trigger oligonucleotide+a herbicide compound and a fungicide compound and an insecticide compound (for example, lambda-cyhalothrin, Warrier®). The container may further provide instructions on the effective use of the mixture. Containers of the present invention can be of any material that is suitable for the storage of the chemical mixture. Containers of the present invention can be of any material that is suitable for the shipment of the chemical mixture. The material can be of cardboard, plastic, metal, or a composite of these materials. The container can have a volume of 0.5 liter, 1 liter, 2 liter, 3-5 liter, 5-10 liter, 10-20 liter, 20-50 liter or more depending upon the need. A tank mix of a trigger oligonucleotide+herbicide compound and a fungicide compound is provided, methods of application to the crop to achieve an effective dose of each compound are known to those skilled in the art and can be refined and further developed depending on the crop, weather conditions, and application equipment used.

Insecticides, fungicides, nematocides, bactericides, acaricides, growth regulators, chemosterilants, semiochemicals, repellents, attractants, pheromones, feeding stimulants or other biologically active compounds can be added to the trigger oligonucleotide to form a multi-component pesticide giving an even broader spectrum of agricultural protection. Examples of such agricultural protectants with which compounds of this invention can be formulated are: insecticides such as abamectin, acephate, azinphos-methyl, bifenthrin, buprofezin, carbofuran, chlorfenapyr, chlorpyrifos, chlorpyrifos-methyl, cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin, deltamethrin, diafenthiuron, diazinon, diflubenzuron, dimethoate, esfenvalerate, fenoxycarb, fenpropathrin, fenvalerate, fipronil, flucythrinate, tau-fluvalinate, fonophos, imidacloprid, isofenphos, malathion, metaldehyde, methamidophos, methidathion, methomyl, methoprene, methoxychlor, methyl 7-chloro-2,5-dihydro-2-[[N-(methoxycarbonyl)-N-[4-(trifluoromethoxy)phenyl]amino]carbonyl]indeno[1,2-e][1,3,4]oxadiazine-4a(3H)-carboxylate (DPX-JW062), monocrotophos, oxamyl, parathion, parathion-methyl, permethrin, phorate, phosalone, phosmet, phosphamidon, pirimicarb, profenofos, rotenone, sulprofos, tebufenozide, tefluthrin, terbufos, tetrachlorvinphos, thiodicarb, tralomethrin, trichlorfon and triflumuron; most preferably a PPG oxidase inhibitor compound is formulated with a fungicide compound or combinations of fungicides, such as azoxystrobin, benomyl, blasticidin-S, Bordeaux mixture (tribasic copper sulfate), bromuconazole, captafol, captan, carbendazim, chloroneb, chlorothalonil, copper oxychloride, copper salts, cymoxanil, cyproconazole, cyprodinil (CGA 219417), diclomezine, dicloran, difenoconazole, dimethomorph, diniconazole, diniconazole-M, dodine, edifenphos, epoxiconazole (BAS 480F), famoxadone, fenarimol, fenbuconazole, fenpiclonil, fenpropidin, fenpropimorph, fluazinam, fluquinconazole, flusilazole, flutolanil, flutriafol, folpet, fosetyl-aluminum, furalaxyl, hexaconazole, ipconazole, iprobenfos, iprodione, isoprothiolane, kasugamycin, kresoxim-methyl, mancozeb, maneb, mepronil, metalaxyl, metconazole, S-methyl 7-benzothiazolecarbothioate (CGA 245704), myclobutanil, neo-asozin (ferric methanearsonate), oxadixyl, penconazole, pencycuron, probenazole, prochloraz, propiconazole, pyrifenox, pyroquilon, quinoxyfen, spiroxamine (KWG4168), sulfur, tebuconazole, tetraconazole, thiabendazole, thiophanate-methyl, thiram, triadimefon, triadimenol, tricyclazole, trifloxystrobin, triticonazole, validamycin and vinclozolin; combinations of fungicides are common for example, cyproconazole and azoxystrobin, difenoconazole, and metalaxyl-M, fludioxonil and metalaxyl-M, mancozeb and metalaxyl-M, copper hydroxide and metalaxyl-M, cyprodinil and fludioxonil, cyproconazole and propiconazole; commercially available fungicide formulations for control of Asian soybean rust disease include, but are not limited to Quadris® (Syngenta Corp), Bravo® (Syngenta Corp), Echo 720® (Sipcam Agro Inc), Headline® 2.09EC (BASF Corp), Tilt® 3.6EC (Syngenta Corp), PropiMax™ 3.6EC (Dow AgroSciences), Bumper® 41.8EC (MakhteshimAgan), Folicur® 3.6F (Bayer CropScience), Laredo® 25EC (Dow AgroSciences), Laredo™ 25EW (Dow AgroSciences), Stratego® 2.08F (Bayer Corp), Domark™ 125SL (Sipcam Agro USA), and Pristine® 38% WDG (BASF Corp) these can be combined with PPG oxidase inhibitor compositions as described in the present invention to provide enhanced protection from fungal disease; nematocides such as aldoxycarb and fenamiphos; bactericides such as streptomycin; acaricides such as amitraz, chinomethionat, chlorobenzilate, cyhexatin, dicofol, dienochlor, etoxazole, fenazaquin, fenbutatin oxide, fenpropathrin, fenpyroximate, hexythiazox, propargite, pyridaben and tebufenpyrad; and biological agents such as Bacillus thuringiensis, Bacillus thuringiensis delta endotoxin, baculovirus, and entomopathogenic bacteria, virus and fungi. 

What is claimed is:
 1. A method for potentiating activity of a protoporphyrinogen IX oxidase (PPG oxidase) inhibitor herbicide in a field comprising (a) applying to the surfaces of plants in the field a composition comprising at least one non-transcribable polynucleotide and a transfer agent, wherein the at least one non-transcribable polynucleotide is from 21 to about 700 nucleotides in length and is identical or complementary to at least 21 contiguous nucleotides of a protoporphyrinogen IX oxidase (PPG oxidase) gene sequence or an RNA sequence thereof, wherein the PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs: 7-12, 14, 15, 17, 20, 29-34, 36-48, 51-57, 61-75, 88, 89, 104, 105, 120-129, 132, 133, 142, 143, 146-149, 162, 163, 166-171, 174, 175, 196-423, 430, 431, 436, 437, 440-523, 534, 535, 550, 551, 556, 557, 560, 561, 564-567, 576-581, 584, 585, 594, 595, 610-613, 626, 627, 630-737, 740-743, 746-777, 780-803, 806-1281, 1284-1287, 1290-1297, 1300, 1301, 1308-1381, 1383, 1384, 1386-1388, 1390-1393, 1395-1397, 1399, 1401, 1402, 1405, 1406, 1410, 1411, 1414-1417, 1420, 1422, 1424, 1425, 1427, 1430, 1432, 1437, 1439, 1440, 1442, 1443, 1446, 1447, 1449, 1453, 1456, 1458, 1459, 1461, 1463-1465, 1467, 1468, 1472, 1473, 1477, 1479-1484, 1487, 1489, 1490, 1495, 1497, 1498, 1499, 1501-1504, 1509, 1512, 1513, 1518, 1519, 1520, 1522, 1524, 1526, 1528, 1529, 1532-1534, 1537-1541, 1545-1548, 1550, 1553, 1556, 1557, 1559, 1562, 1563, 1565, 1566, 1568, 1573, 1574, 1576, 1578-1580, 1583, 1584, 1586, 1587, 1590, 1592, 1594, 1598, 1599, 1601, 1602, 1604-1607, 1610, 1611, 1616, 1618, 1619, 1624, 1625, 1627-1629, 1632, 1633, 1635, 1636, 1639, 1641, 1644, 1647, 1648, 1651-1653, 1655-1658, 1661, 1664-1669, 1671, 1672, 1674, 1675, 1677, 1679, 1681, 1683, 1685, 1687, 1688, 1692, 1695, 1698, 1699, 1700, 1703, 1704, 1706, 1708-1710, 1713-1715, 1718, 1719, 1721, 1722, 1724, 1726-1729, 1731, 1732, 1735, 1738, 1740, 1742, 1745, 1748, 1750-1752, 1754, 1755, 1759-1763, 1765, 1766, 1768, 1772-1776, 1782, 1785-1787, 1791-1793, 1795-1797, 1801, 1802, 1804, 1805, 1808, 1810, 1811, 1815, 1820, 1823, 1825, 1826, 1829-1833, 1836-1838, 1840, 1842, 1845-1847, 1849-1852, 1854-1856, 1858, 1868-1870, 1873-1876, 1878-1880, 1883, 1885, 1886, 1891, 1893, 1895-1897, 1900, 1902, 1909, 1912-1914, 1916, 1917, 1920, 1921, 1923-1926, 1928, 1929, 1931-1933, 1936, 1939, 1940, 1944, 1946, 1950-1952, 1954, 1959, 1961-1963, 1965, 1968, 1970, 1972, 1977, 1980-1983, 1985, 1988, 1991-1995, 1997-1999, 2003, 2008-2010, 2013, 2014, 2016, 2020, 2021, 2025-2031, 2033-2035, 2038, 2040-2042, 2044, 2048, 2051-2053, 2056, 2059, 2060, 2063-2065, 2069, 2071, 2074, 2076, 2077, 2079, 2080, 2082, 2085-2087, 2093-2098, 2102-2104, 2106, 2108, 2111-2115, 2117, 2118, 2122, 2129, 2131, 2132, 2134, 2136, 2138-2140, 2142, 2147, 2152-2154, 2156, 2159, 2160, 2161, 2164, 2168, 2171, 2174, 2176, 2177, 2179-2181, 2183-2185, 2189, 2191, 2194, 2196, 2197, 2199, 2203-2205, and 2209-2213, wherein the transfer agent conditions the surfaces of the plants in the field for permeation by the at least one non-transcribable polynucleotide, and (b) applying the PPG oxidase inhibitor herbicide to the plants in the field; whereby the at least one non-transcribable polynucleotide permeates the interior of the plants and induces suppression of a PPG oxidase gene, thereby potentiating activity of the PPG oxidase inhibitor herbicide in the plants.
 2. The method of claim 1, wherein the at least one non-transcribable polynucleotide is selected from the group consisting of a sense single-stranded DNA (ssDNA), an anti-sense ssDNA, a sense single-stranded RNA (ssRNA), an anti-sense ssRNA, a double-stranded RNA (dsRNA), a double-stranded DNA (dsDNA), and a dsDNA/RNA hybrid; or the composition comprises two or more of the at least one non-transcribable polynucleotides.
 3. The method of claim 1, wherein the transfer agent is an organosilicone surfactant composition or an organosilicone compound contained therein.
 4. The method of claim 1, wherein the composition further comprises the PPG oxidase inhibitor herbicide.
 5. The method of claim 4, wherein the PPG oxidase inhibitor herbicide is selected from the group consisting of acifluorfen-Na, bifenox, chlomethoxyfen, chlornitrofen, ethoxyfen-ethtyl, fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen, fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin, flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon, oxadiargyl, pyraclonil, flufenpyr-ethyl, azafenidin, carfentrazone-ethyl, saflufenacil, sulfentrazone, pentoxazone, benzfendizone, butafenacil, pyrazogyl, and profluazol.
 6. The method of claim 4, wherein the composition further comprises one or more herbicides different from the PPG oxidase inhibitor herbicide.
 7. The method of claim 4, wherein the composition further comprises a co-herbicide.
 8. The method of claim 7, wherein the co-herbicide is selected from the group consisting of amide herbicides, arsenical herbicides, benzothiazole herbicides, benzoylcyclohexanedione herbicides, benzofuranyl alkylsulfonate herbicides, cyclohexene oxime herbicides, cyclopropylisoxazole herbicides, dicarboximide herbicides, dinitroaniline herbicides, dinitrophenol herbicides, dithiocarbamate herbicides, glycine herbicides, halogenated aliphatic herbicides, imidazolinone herbicides, inorganic herbicides, nitrile herbicides, organophosphorus herbicides, oxadiazolone herbicides, oxazole herbicides, phenoxy herbicides, phenylenediamine herbicides, pyridazine herbicides, pyridazinone herbicides, pyridine herbicides, pyrimidinediamine herbicides, pyrimidinyloxybenzylamine herbicides, quaternary ammonium herbicides, thiocarbamate herbicides, thiocarbonate herbicides, thiourea herbicides, triazine herbicides, triazinone herbicides, triazolone herbicides, triazolopyrimidine herbicides, uracil herbicides, and urea herbicides.
 9. The method of claim 4, wherein the composition further comprises a pesticide.
 10. The method of claim 9, wherein the pesticide is selected from the group consisting of insecticides, fungicides, nematicides, bactericides, acaricides, growth regulators, chemosterilants, semiochemicals, repellents, attractants, pheromones, feeding stimulants, and biopesticides.
 11. The method of claim 1, wherein the plants are selected from the group consisting of Amaranthus albus, Amaranthus graecizans, Amaranthus hybridus, Amaranthus lividus, Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus, Amaranthus thunbergii, Amaranthus viridis, Ambrosia trifida, Chenopodium album, Commelina diffusa, Conyza canadensis, Digitaria sanguinalis, Euphorbia heterophylla, Kochia scoparia, and Lolium multiflorum.
 12. A method of reducing growth, development, or reproductive ability in a plant, comprising treating the surfaces of a plant in a field with a composition comprising a PPG oxidase inhibitor herbicide in combination with (a) at least one non-transcribable polynucleotide that is from 21 to about 700 nucleotides in length and is identical or complementary to at least 21 contiguous nucleotides of a PPG oxidase gene sequence or an RNA sequence thereof, wherein the PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs: 7-12, 14, 15, 17, 20, 29-34, 36-48, 51-57, 61-75, 88, 89, 104, 105, 120-129, 132, 133, 142, 143, 146-149, 162, 163, 166-171, 174, 175, 196-423, 430, 431, 436, 437, 440-523, 534, 535, 550, 551, 556, 557, 560, 561, 564-567, 576-581, 584, 585, 594, 595, 610-613, 626, 627, 630-737, 740-743, 746-777, 780-803, 8006-1281, 1284-1287, 1290-1297, 1300, 1301, 1308-1381, 1383, 1384, 1386-1388, 1390-1393, 1395-1397, 1399, 1401, 1402,1405, 1406, 1410, 1411, 1414-1417, 1420, 1422, 1424, 1425, 1427, 1430, 1432, 1437, 1439, 1440, 1442, 1443, 1446, 1447, 1449, 1453, 1456, 1458, 1459, 1461, 1463-1465, 1467, 1468, 1472, 1473, 1477, 1479-1484, 1487, 1489, 1490, 1495, 1497, 1498, 1499, 1501-1504, 1509, 1512, 1513, 1518, 1519, 1520, 1522, 1524, 1526, 1528, 1529, 1532-1534, 1537-1541, 1545-1548, 1550, 1553, 1556, 1557, 1559, 1562, 1563, 1565, 1566, 1568, 1573, 1574, 1576,1578-1580, 1583, 1584, 1586, 1587, 1590, 1592, 1594, 1598, 1599, 1601, 1602, 1604-1607, 1610, 1611, 1616, 1618, 1619, 1624, 1625, 1627-1629, 1632, 1633, 1635, 1636, 1639, 1641, 1644, 1647, 1648, 1651-1653, 1655-1658, 1661, 1664-1669, 1671, 1672, 1674, 1675, 1677, 1679, 1681, 1683, 1685, 1687, 1688, 1692, 1695, 1698, 1699, 1700, 1703, 1704, 1706, 1708-1710, 1713-1715, 1718, 1719, 1721, 1722, 1724, 1726-1729, 1731, 1732, 1735, 1738, 1740, 1742, 1745, 1748, 1750-1752, 1754, 1755, 1759-1763, 1765, 1766, 1768, 1772-1776, 1782, 1785-1787, 1791-1793, 1795-1797, 1801, 1802, 1804, 1805, 1808, 1810, 1811, 1815, 1820, 1823, 1825, 1826, 1829-1833, 1836-1838, 1840, 1842, 1845-1847, 1849-1852, 1854-1856, 1858, 1868-1870, 1873-1876, 1878-1880,1883, 1885, 1886, 1891, 1893, 1895-1897, 1900, 1902, 1909, 1912-1914, 1916, 1917, 1920, 1921, 1923-1926, 1928, 1929, 1931-1933, 1936, 1939, 1940, 1944, 1946, 1950-1952, 1954, 1959, 1961-1963, 1965, 1968, 1970, 1972, 1977, 1980-1983, 1985, 1988, 1991-1995, 1997-1999, 2003, 2008-2010, 2013, 2014, 2016, 2020, 2021, 2025-2031, 2033-2035, 2038, 2040-2042, 2044, 2048, 2051-2053, 2056, 2059, 2060, 2063-2065, 2069, 2071, 2074, 2076, 2077, 2079, 2080, 2082, 2085-2087, 2093-2098, 2102-2104, 2106, 2108, 2111-2115, 2117, 2118, 2122, 2129, 2131, 2132, 2134, 2136, 2138-2140, 2142, 2147, 2152-2154, 2156, 2159, 2160, 2161, 2164, 2168, 2171, 2174, 2176, 2177, 2179-2181, 2183-2185, 2189, 2191, 2194, 2196, 2197, 2199, 2203-2205, and 2209-2213, and (b) an organosilicone surfactant, whereby growth, development, or reproductive ability is reduced in the treated plant compared to a control plant treated with the PPG oxidase inhibitor herbicide alone.
 13. The method of claim 12, wherein the at least one non-transcribable polynucleotide is selected from the group consisting of a sense ssDNA, an anti-sense ssDNA, a sense ssRNA, an anti-sense ssRNA, a dsRNA, a dsDNA, and a dsDNA/RNA hybrid; or the composition comprises two or more of the at least one non-transcribable polynucleotides.
 14. The method of claim 12, wherein the PPG oxidase inhibitor herbicide is selected from the group consisting of acifluorfen-Na, bifenox, chlomethoxyfen, chlornitrofen, ethoxyfen-ethtyl, fluoroglycofen-ethyl, fomesafen, halosafen, lactofen, oxyfluorfen, fluazolate, pyraflufen-ethyl, cinidon-ethyl, flumioxazin, flumiclorac-pentyl, fluthiacet-methyl, thidiazimin, oxadiazon, oxadiargyl, pyraclonil, flufenpyr-ethyl, azafenidin, carfentrazone-ethyl, saflufenacil, sulfentrazone, pentoxazone, benzfendizone, butafenacil, pyrazogyl, and profluazol.
 15. The method of claim 12, wherein the composition further comprises a co-herbicide or a pesticide.
 16. The method of claim 12, wherein the plant is selected from the group consisting of Amaranthus albus, Amaranthus graecizans, Amaranthus hybridus, Amaranthus lividus, Amaranthus palmeri, Amaranthus rudis, Amaranthus spinosus, Amaranthus thunbergii, Amaranthus viridis, Ambrosia trifida, Chenopodium album, Commelina diffusa, Conyza canadensis, Digitaria sanguinalis, Euphorbia heterophylla, Kochia scoparia, and Lolium multiflorum.
 17. The method of claim 1, wherein the PPG oxidase gene sequence is selected from the group consisting of SEQ ID NOs: 72-75, 88, 89, 104, 105, 120-129, 132, 133, 142, 143, 146-149, 162, 163, 168-171, 174, 175, 430, 431, 522, 523, 534, 535, 550, 551, 556, 557, 560, 561, 564-567, 576-581, 584, 585, 594, 595, 610-613, 626, 627, 734-737, 740-743, 746-751, 1383-1384, 1386-1388, 1390-1393, 1395-1397, 1399, 1401-1402, 1405-1406, 1410-1411, 1414-1417, 1420,1422, 1424-1425, 1427, 1430, 1432, 1437, 1439-1440, 1442-1443, 1446-1447, 1449, 1453, 1456, 1458, 1459, 1461, 1463-1465, 1467, 1468, 1472, 1473, 1477, 1479-1484, 1487, 1489-1490, 1495, 1497-1499, 1501-1504, 1509, 1512, 1513, 1518-1520, 1522, 1524, 1526, 1528, 1529, 1532-1534, 1537-1541, 1545-1548, 1550, 1553, 1556, 1557, 1559, 1562, 1563, 1565, 1566, 1568, 1573, 1574, 1576, 1578-1580, 1583, 1584, 1586, 1587, 1590, 1592, 1594, 1598, 1599, 1601, 1602, 1604-1607, 1610, 1611, 1616, 1618, 1619, 1624, 1625, 1627-1629, 1632, 1633, 1635, 1636, 1639, 1641, 1644, 1647, 1648, 1651-1653, 1655-1658, 1661, 1664-1669, 1671, 1672, 1674, 1675, 1677, 1679, 1681, 1683, 1685, 1687, 1688, 1692, 1695, 1698-1700, 1703, 1704, 1706, 1708-1710, 1713-1715, 1718, 1719, 1721, 1722, 1724, 1726-1729, 1731, 1732, 1735, 1738, 1740, 1742, 1745, 1748, 1750-1752, 1754, 1755, 1759-1763, 1765, 1766, 1768, 1772-1776, 1782, 1785-1787, 1791-1793, 1795-1797, 1801, 1802, 1804, 1805, 1808, 1810, 1811, 1815, 1820, 1823, 1825, 1826, 1829-1833, 1836-1838, 1840, 1842, 1845-1847, 1849-1852, 1854-1856, 1858, 1868-1870, 1873-1876, 1878-1880, 1883, 1885, 1886, 1891, 1893, 1895-1897, 1900, 1902, 1909, 1912-1914, 1916, 1917, 1920, 1921, 1923-1926, 1928, 1929, 1931-1933, 1936, 1939, 1940, 1944, 1946, 1950-1952, 1954, 1959, 1961-1963, 1965, 1968, 1970, 1972, 1977, 1980-1983, 1985, 1988, 1991-1995, 1997-1999, 2003, 2008-2010, 2013, 2014, 2016, 2020, 2021, 2025, 2031, 2033-2035, 2038, 2040-2042, 2044, 2048, 2051-2053, 2056, 2059, 2060, 2063-2065, 2069, 2071, 2074, 2076, 2077, 2079, 2080, 2082, 2085-2087, 2093-2098, 2102-2104, 2106, 2108, 2111-2115, 2117, 2118, 2122, 2129, 2131, 2132, 2134, 2136, 2138-2140, 2142, 2147, 2152-2154, 2156, 2159-2161, 2164, 2168, 2171, 2174, 2176, 2177, 2179-2181, 2183-2185, 2189, 2191, 2194, 2196-2197, 2199, 2203-2205, and 2209-2213.
 18. The method of claim 1, wherein the transfer agent is a surfactant.
 19. The method of claim 1, wherein the applying the PPG oxidase inhibitor herbicide to the plants of step (b) is at least one day after the applying to the surface of the plants the composition of step (a). 